Method and device for screening primer design template and application

A primer design and template technology, applied in the fields of bioinformatics, instrumentation, genomics, etc., can solve the problems of high subjectivity, high requirements for designers' experience, and tediousness, etc., and achieve the goal of simple primer design, convenient primer design, and simplified difficulty Effect
CN110970093AActive Publication Date: 2020-04-07深圳华大因源医药科技有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
深圳华大因源医药科技有限公司
Publication Date
2020-04-07

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Abstract

The invention discloses a method and device for screening a primer design template and an application. The method comprises a microbial genome database arrangement step, a host genome database arrangement step, a primer design template database generation step, a primer design template consensus rate evaluation step, a primer design template specificity evaluation step and a primer design templateoutput step. The method is simple and convenient to operate, and primer design templates can be screened in batches; the device for screening the primer design template is high in automation degree,high in operation speed, low in cost and capable of efficiently and accurately outputting the primer design templates of one or more species in batches. The method and the device provided by the invention have the advantage of high precision, the screened primer design template has the characteristics of high consensus rate and high specificity, the target detection primer or probe with strong applicability and good specificity can be designed, and a solid foundation is laid for the technical scheme of detection based on the primer or probe.
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Description

technical field

[0001] The present application relates to the field of primer design methods, in particular to a method, device and application for screening primer design templates. Background technique

[0002] In the field of microbial detection, at present, it mainly relies on the traditional culture method for detection, and the traditional culture method takes a long time and has a high rate of missed detection. In addition to culture methods, molecular biology methods have gradually been widely used. With the application of molecular biology technology, microbial detection technology has progressed from biological inspection to molecular biology identification. Different species of pathogenic microorganisms have different genes or base sequences, so it can be detected by detecting the specific gene sequence of pathogenic microorganisms. presence or absence is detected. Commonly used methods mainly include nucleic acid hybridization and polymerase chain reaction (PCR...

Claims

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