Sample loading method, sequencing method and sequencing system for high-throughput sequencing

Abstract

The invention relates to a sample loading method, a sequencing method and a sequencing system for high-throughput sequencing. The sample loading method comprises the following steps: preparing a sample to be loaded into fog drops, and then spraying the fog drops on the surface of a chip for high-throughput sequencing to enable the fog drops to be combined with the chip so as to obtain the chip loaded with the sample. The sample loading method is a non-pipeline fluid loading scheme, can avoid the problem of non-uniform sample loading and improve the data output, is low in the demand on samples,and can avoid the problem of exogenous impurities.

Description

technical field [0001] The invention relates to the technical field of sequencing, in particular to a sample loading method, a sequencing method and a sequencing system used in high-throughput sequencing. Background technique [0002] DNA sequencing technologies include first-generation DNA sequencing technologies represented by Sanger sequencing and second-generation DNA sequencing technologies represented by Illumina Hiseq2500, Roche 454, ABI Solid, BGISEQ-500, etc. The Sanger sequencing method has the characteristics of simple experimental operation, intuitive and accurate results, and short experimental cycle. It is widely used in the fields of clinical gene mutation detection and genotyping, which require high timeliness of detection results. [0003] Compared with the first-generation DNA sequencing technology, the second-generation DNA sequencing technology requires special processing of the target sequence before the sequence is read, so that the fragments to be test...

AI Technical Summary

Problems solved by technology

Pipeline fluid loading technology has the following defects: the cover plate covering the surface of the bare chip has the risk of carrying impurities, which is easy to adsorb impurities on the surface of the chip, directly affecting the quality and throughput of sequencing; The amount of reagents produced different exchange times on different chips, which affects the sequencing quality of DNB loading; in order to meet the full exchange of reagents, the amount of reagents used is large and the utilization rate is low, which increases the time cost and biochemical cost of DNB loading process to a certain extent. Reagent costs, and the edge area of ​​the chip is prone to the risk of uneven DNB loading

However, there is no application of non-pipeline fluid loading technology in second-generation DNA sequencing technology, especially there is no precedent for this loading technology to load DNB onto the surface of a sequencing chip

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