Sample loading method, sequencing method and sequencing system for high-throughput sequencing

A high-throughput, sample technology, applied in the field of sequencing, can solve the problems affecting sequencing quality and throughput, uneven DNB loading, carrying impurities, etc., to improve sequencing quality and data output, and avoid the problem of uneven sample loading. , the effect of avoiding the problem of exogenous impurities

Pending Publication Date: 2020-04-21
SHENZHEN HUADA GENE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Pipeline fluid loading technology has the following defects: the cover plate covering the surface of the bare chip has the risk of carrying impurities, which is easy to adsorb impurities on the surface of the chip, directly affecting the quality and throughput of sequencing; The amount of reagents produced different exchange times on different chips, which affects the sequencing quality of DNB loading; in order to meet the full exchange of reagents, the amount of re

Method used

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  • Sample loading method, sequencing method and sequencing system for high-throughput sequencing
  • Sample loading method, sequencing method and sequencing system for high-throughput sequencing

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Experimental program
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Embodiment

[0064] This embodiment is a DNB sample loading method with a regular matrix bare chip based on the DIP series sequencer platform developed by BGI. The sample loading method uses an atomizer to prepare the DNB to be loaded into droplets and spray it on the surface of the bare chip with a regular matrix to complete the sample loading process, and then the sample loaded by this method is performed on the DIP sequencer developed by BGI. detection.

[0065] The nebulizer used in this example is an OMRON Comp-A-I-R nebulizer, the diameter of the generated mist droplets is about 2 μm, and the atomization rate in a steady state is about 229.00 μL / min.

[0066] The chip used in this embodiment is a bare chip with a regular matrix, which comes from Huada Intelligent Manufacturing Co., Ltd. The chip specification is DipseqV2, 25mm*75mm, and the pitch value (chip matrix point spacing) is 900nm.

[0067] In order to confirm that this embodiment can reduce the demand of DNB samples, firstl...

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Abstract

The invention relates to a sample loading method, a sequencing method and a sequencing system for high-throughput sequencing. The sample loading method comprises the following steps: preparing a sample to be loaded into fog drops, and then spraying the fog drops on the surface of a chip for high-throughput sequencing to enable the fog drops to be combined with the chip so as to obtain the chip loaded with the sample. The sample loading method is a non-pipeline fluid loading scheme, can avoid the problem of non-uniform sample loading and improve the data output, is low in the demand on samples,and can avoid the problem of exogenous impurities.

Description

technical field [0001] The invention relates to the technical field of sequencing, in particular to a sample loading method, a sequencing method and a sequencing system used in high-throughput sequencing. Background technique [0002] DNA sequencing technologies include first-generation DNA sequencing technologies represented by Sanger sequencing and second-generation DNA sequencing technologies represented by Illumina Hiseq2500, Roche 454, ABI Solid, BGISEQ-500, etc. The Sanger sequencing method has the characteristics of simple experimental operation, intuitive and accurate results, and short experimental cycle. It is widely used in the fields of clinical gene mutation detection and genotyping, which require high timeliness of detection results. [0003] Compared with the first-generation DNA sequencing technology, the second-generation DNA sequencing technology requires special processing of the target sequence before the sequence is read, so that the fragments to be test...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/6837
CPCC12Q1/6869C12Q1/6837C12Q2535/122
Inventor 杨晋徐讯章文蔚徐崇钧陈奥陈莹崔兴业
Owner SHENZHEN HUADA GENE INST
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