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Sample treatment device, sample treatment method, and digital PCR system comprising sample treatment device

A technology for processing devices and samples, applied in the fields of digital PCR systems and droplet digital PCR systems, can solve the problems of unsuitable integrated droplet digital PCR systems, not compact enough, and large volume.

Active Publication Date: 2020-04-24
BEIJING ZHIYU BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these devices are usually complex in structure, not compact enough, bulky, and complicated for users to operate, and are not suitable for building an integrated droplet digital PCR system

Method used

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  • Sample treatment device, sample treatment method, and digital PCR system comprising sample treatment device
  • Sample treatment device, sample treatment method, and digital PCR system comprising sample treatment device
  • Sample treatment device, sample treatment method, and digital PCR system comprising sample treatment device

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Experimental program
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Effect test

Embodiment 1

[0069] Such as Figure 1 to Figure 3 As shown, a sample processing device includes a sample processing consumable 200, a driving module 100 connected to the sample processing consumable 200, and a control module (not shown in the figure), the driving module 100 is a device for driving liquid flow, driving The module 100 is connected to and controlled by the control module.

[0070] The sample processing consumable 200 includes a substrate 210 and a reagent consumable 230, which are detachably connected. Further, the sample processing consumable 200 includes a first base material part 211 , a second base material part 212 , and an elongated connecting part 270 whose two ends are respectively connected to the first base material part 211 and the second base material part 212 . A plurality of extraction cavities 260 are provided on the first base material part 211 at intervals along its length direction, and a sample cavity 250 is formed on the second base material part 212 . T...

Embodiment 2

[0086] This embodiment provides a sample processing device, which is basically the same as Embodiment 1. In this embodiment: the specific design and layout of the driving module used to drive the liquid flow in each cavity of the sample processing consumable 200 and the driving module of Embodiment 1 different. Such as Figure 5 , which shows four groups of drive modules. Each drive module includes an air pump (not shown in the figure, the air pump interface is connected to the air intake interface 131 in the figure through a gas pipeline), a gas sensor (installed inside the connection seat 170, not shown in the figure), a pneumatic valve group 160 and gas pipeline (not shown in the figure). The pneumatic valve group 160 includes a valve base 180 fixedly connected to the connecting seat 170 and a plurality of valves 182 (corresponding to the extraction cavity and the sample cavity) arranged on the valve base 180 . The inside of the valve base 180 is provided with a channel ...

Embodiment 3

[0088] This embodiment provides a digital PCR system, such as Figure 7 As shown, the system includes a base 1, a moving mechanism arranged on the base 1, an operating platform 2 arranged on the base, a droplet generating device 3 with a sampling needle 300, a nucleic acid amplification temperature control device, and a product signal A collection device 8, a control device (not shown in the figure), and a sample processing device as shown in Embodiment 2. The moving mechanism, the sample processing device, the droplet generating device 3, the nucleic acid amplification temperature control device, and the product signal collecting device 8 are respectively connected to and controlled by the control device.

[0089] Specifically, the moving mechanism includes an X guide rail 91 extending along the length direction of the micro digital PCR system, a mobile base frame 92 slidingly connected with the X guide rail, and a mobile base frame 92 arranged on the mobile base frame 92 and...

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Abstract

The invention discloses a sample treatment device, a sample treatment method, and a digital PCR system comprising the sample treatment device. The sample treatment device comprises a sample treatmentconsumable material, a driving module connected with the sample treatment consumable material, and a control module, wherein the sample treatment consumable material comprises a base material, a plurality of extraction cavities, a sample cavity, a transfer channel arranged between the sample cavity and the extraction cavities and used for communicating the sample cavity and the extraction cavities, and a reagent consumable material with a reagent storage cavity, the bottoms of the extraction cavities are communicated through a micro-channel, and the reagent storage cavity is communicated withthe sample cavity through the micro-channel. The sample treatment device has multiple functions such as nucleic acid extraction, reagent mixing and the like, is suitable for obtaining samples directlyfor digital PCR detection, is simple and compact in structure and small in size, can be designed in a modular mode, and is suitable for constructing an integrated droplet type digital PCR system.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a sample processing device and method suitable for digital PCR detection, and a digital PCR system including the sample processing device, especially a droplet digital PCR system. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs rapid and accurate detection methods, among which molecular detection has unique advantages. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology. Molecular detection products are mainly used in the detection of various clinical subjects such as tumors, infections, genetics, and prenatal screening, as well as in medical examination centers, technical service centers, third-party testing institutions, and rapi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42C12M1/38C12M1/36C12M1/34C12Q1/6806C12Q1/6851
CPCB01L7/52C12Q1/6806C12Q1/6851B01L2400/0487C12Q2563/143C12Q2563/149C12Q2527/125C12Q2563/159C12M1/38C12M1/36C12M1/34C12M1/00C12M1/42C12Q1/68
Inventor 李昂
Owner BEIJING ZHIYU BIOTECH LTD
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