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A long subspecies of Bifidobacterium longum and its application

A technology of Bifidobacterium longum and subspecies, applied in the directions of bifidobacteria, applications, bacteria, etc., can solve problems such as neuronal function damage, and achieve the effect of relieving social barriers, improving constipation, and improving cognitive ability

Active Publication Date: 2022-04-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous activity and proliferation of glial cells (astrocytes and microglia) with morphological changes, the release of various pro-inflammatory mediators (cytokines and chemokines) to coordinate immune responses and regulate neuronal function and connectivity, while this response can be neuroprotective (i.e., help repair damaged tissue), chronic dysregulation of CNS cytokines can lead to disruption of neuronal function through their roles in cognition and synaptic regulation, These cytokines include IL-6 and TNF-α, etc.

Method used

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  • A long subspecies of Bifidobacterium longum and its application
  • A long subspecies of Bifidobacterium longum and its application
  • A long subspecies of Bifidobacterium longum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Bifidobacterium longum subspecies CCFM1077 has good tolerance to simulated gastrointestinal fluid

[0069] The cryopreserved Bifidobacterium longum subsp. longum CCFM1077 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and then subcultured in mMRS medium After 2 to 3 times, take 1 mL of the culture solution of Bifidobacterium longum subsp. longum CCFM1077, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and incubate at 37°C. For anaerobic culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0070] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria a...

Embodiment 2

[0076] Example 2: Bifidobacterium longum subspecies CCFM1077 has no toxic side effects on wistar rats

[0077] Bifidobacterium longum subsp. longum CCFM1077 was resuspended in 10% skim milk to make a concentration of 3.0×10 8 CFU / mL bacterial suspension. Six male wistar autistic rats with a body weight of about 200 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0078] The results showed that the feeding concentration of 3.0×10 8 CFU / mL of Bifidobacterium longum subsp. longum CCFM1077 had no significant effect on rats, no significant change in body weight, and no death. The appearance of the rats showed no obvious pathological symptoms.

Embodiment 3

[0079] Example 3: Bifidobacterium longum subspecies longum CCFM1077 can significantly improve the autonomous exploration ability of autistic rats

[0080] (1) Breeding of young mice with autism

[0081] Healthy male and female wistar rats were bred and adapted to the environment for 1 week. They were caged together at 5:00 p.m. at a ratio of 2:1, and the vaginal plug was observed at 8:00 the next morning. If no vaginal plug was observed, a vaginal smear was performed. On the first day of pregnancy, the female mice were housed in separate cages after conception, and randomly divided into model group and control group. On the 12.5th day after pregnancy, intraperitoneal injection of sodium valproate (VPA) 600mg / kg (normal saline made into 250mg / mL solution) was given as the model group; Weaning.

[0082]

[0083] (2) Experimental grouping of autistic rats

[0084] Divided into control group, model group and intervention group.

[0085]

[0086] (3) Open field test of au...

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Abstract

The invention discloses a Bifidobacterium longum longum subspecies and an application thereof. Bifidobacterium longum longum subspecies CCFM1077 can improve autonomous behaviors and exploratory behaviors in unfamiliar environments caused by autism, and relieve social interaction caused by autism. It can significantly improve the stereotyped behavior caused by autism, improve the cognitive ability of autism to new objects, improve constipation, and effectively adsorb polychlorinated biphenyls.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to Bifidobacterium longum subsp. longum and application thereof. Background technique [0002] Autism (Autism), also known as autism, has been recognized as a generalized neurodevelopmental disorder that frequently occurs in infants and young children, mainly manifested in social interaction impairment, communication (verbal and non-verbal) function impairment, and repetitive stereotyped behaviors. , often accompanied by a variety of clinical symptoms such as mental retardation, epilepsy or hyperactivity, usually before the age of 3. According to the latest data released by the US Centers for Disease Control and Prevention (CDC), the prevalence of ASD in 2018 was 1:59, a 15% increase from 1:68 in 2016, and the prevalence rate of men was 4 to 5 times that of women. . [0003] ASD is a highly heterogeneous neurodevelopmental disorder. Genetic factors ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P25/28A23L33/135C12R1/01
CPCA61K35/745A61P25/28A23L33/135A23V2002/00C12R2001/01C12N1/205A23V2400/533A23V2200/3204A23V2200/30
Inventor 陈卫王刚孔庆敏赵建新张灏
Owner JIANGNAN UNIV
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