Automatic collecting, replacing and sampling device for soil microorganism breathing gas

Abstract

The invention discloses an automatic collecting, replacing and sampling device for soil microorganism breathing gas. The device comprises an incubator, a vacuum pump, an air compressor, a negative pressure controller, a three-way balance pressure valve group, a three-way gas collection switching valve group, an automatic controller, at least two two-way balance pressure valve groups and two-way gas collection valve groups, culture bottles are communicated with gas inlets of two-way gas collection valves in the two-way gas collection valve groups in a one-to-one correspondence manner; gas outlets of two-way gas collection valves in the same group are communicated with independent gas collection ports of the same three-way gas production switching valve; a shared exhaust port of the three-way gas production switching valve group is communicated with a gas outlet of a ventilation three-way valve, a shared gas inlet of the three-way gas production switching valve group is communicated witha sample injection gas pipe, the sample injection gas pipe is communicated with a gas inlet of a sample injection three-way valve, and a gas outlet of the sample injection three-way valve is used forbeing communicated with a gas analyzer. According to the invention, the automatic collection, replacement and sample introduction of the sample gas can be realized, so that leakage and cross contamination of the sample gas are prevented, and the online continuous monitoring of the sample gas is ensured.

Description

technical field [0001] The invention relates to the technical field of gas collection and replacement, in particular to an automatic collection, replacement and sampling device of soil microbial respiratory gas. Background technique [0002] With the sharp increase of extreme and severe weather, it is becoming increasingly important to further study the global carbon cycle and the source of atmospheric greenhouse gases, so the requirements for the detection of atmospheric greenhouse gases and carbon isotope ratios are also getting higher and higher. Soil microbial respiration produces CO 2 and CH 4 All metabolic activities such as greenhouse gases are the main components of soil respiration, and are hot issues in the study of carbon cycle in terrestrial ecosystems. The stable carbon isotope tracer test analysis technology can accurately quantify soil microbial respiration, and improve the understanding of the carbon cycle process mediated by soil microorganisms and its lea...

AI Technical Summary

Problems solved by technology

At present, there are relatively few commercial soil cultivation and gas collection front-ends on the market, and most of them are designed for field experiment conditions, such as the SF-3000 automatic multi-channel soil gas flux measurement system developed by LICA. Suitable for soil sample culture system, but cannot meet the needs of indoor simulated soil microbial respiration research

However, the soil culture system of the RI-8800 automatic variable temperature soil culture pretreatment device developed by the original ecology company is an open double-loop design, which is likely to cause cross-contamination between marked samples and non-marked samples, affecting the accuracy of the measurement results. Suitable for simultaneous and synchronous monitoring research of soil microbial respiration carbon isotope tracer

Therefore, limited by the lack of a suitable gas collection front-end, research on the online continuous monitoring of soil microbial respiration cannot be carried out.

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