A device for automatic collection, replacement and sampling of soil microbial respiratory gas

Abstract

The invention discloses an automatic collection, replacement and sampling device of soil microbial respiratory gas, which comprises an incubator, a vacuum pump, an air compressor, a negative pressure controller, a three-way balance pressure valve group, a three-way gas collection switching valve group, an automatic The controller and at least two groups of two-way balanced pressure valve groups and two-way gas sampling valve groups, the culture bottle and the inlet ports of the two-way gas sampling valves in the two-way gas sampling valve group are connected one by one. The gas outlets of the two-way gas collection valve are all connected to the independent gas collection port of the same three-way gas collection switching valve, the shared exhaust port of the three-way gas collection switching valve group is connected to the gas outlet of the ventilation three-way valve, and the three-way gas collection The shared air inlet of the switching valve group is connected with a sample gas pipe, the sample gas pipe is connected with the gas inlet of the sample three-way valve, and the gas outlet of the sample three-way valve is used to communicate with the gas analyzer. The invention can realize the automatic collection, replacement and sampling of the sample gas, so as to prevent the leakage and cross-contamination of the sample gas and ensure the online continuous monitoring of the sample gas.

Description

technical field [0001] The invention relates to the technical field of gas collection and replacement, in particular to an automatic collection, replacement and sampling device of soil microbial respiratory gas. Background technique [0002] With the sharp increase of extreme and severe weather, it is becoming increasingly important to further study the global carbon cycle and the source of atmospheric greenhouse gases, so the requirements for the detection of atmospheric greenhouse gases and carbon isotope ratios are also getting higher and higher. Soil microbial respiration produces CO 2 and CH 4 All metabolic activities such as greenhouse gases are the main components of soil respiration, and are hot issues in the study of carbon cycle in terrestrial ecosystems. The stable carbon isotope tracer test analysis technology can accurately quantify soil microbial respiration, and improve the understanding of the carbon cycle process mediated by soil microorganisms and its lea...

AI Technical Summary

Problems solved by technology

At present, there are relatively few commercial soil cultivation and gas collection front-ends on the market, and most of them are designed for field experiment conditions, such as the SF-3000 automatic multi-channel soil gas flux measurement system developed by LICA. Suitable for soil sample culture system, but cannot meet the needs of indoor simulated soil microbial respiration research

However, the soil culture system of the RI-8800 automatic variable temperature soil culture pretreatment device developed by the original ecology company is an open double-loop design, which is likely to cause cross-contamination between marked samples and non-marked samples, affecting the accuracy of the measurement results. Suitable for simultaneous and synchronous monitoring research of soil microbial respiration carbon isotope tracer

Therefore, limited by the lack of a suitable gas collection front-end, research on the online continuous monitoring of soil microbial respiration cannot be carried out.

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