Method for capturing and detecting abnormal metabolic cells in urine

A technology of abnormal metabolism and detection method, which is applied in the field of urine abnormal metabolism cell capture and detection, which can solve the problems of unclear images, inaccurate detection results, and large differences in sedimentation velocity, etc., and achieve the effect of accurate detection results and clear images

Inactive Publication Date: 2020-04-28
HANGZHOU JUNHUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for capturing and detecting cells with abnormal metabolism in urine, which has the advantages of clear images and accurate detection results, and solves the problem of differences in sedimentation velocity due to the different viscosities of urine in current cell capture and detection methods. Very large, it is easy to cause unclear images and inaccurate detection results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Remove sample impurities: take a urine sample, pour the urine sample into a test tube, then add an aqueous urease solution to the test tube, and incubate it to remove the urea in the urine sample, then add an active adsorbent, and use ultrasonic vibration to remove Sulfur and phosphorus in urine samples; adding PBS solution: pour the urine sample into a centrifuge for centrifugation, add PBS solution to obtain urine cells resuspended in FPBS; processing vessels: wash the vessels, add APTES solution to In the container, then place the container in the dark for one and a half hours, then suck off the excess APTES, and finally wash it several times with pure water, then put the container in an oven to dry; pour into the container: urine cells resuspended in FPBS Add it into the container, then suck out the excess liquid, then suck out the excess liquid and add paraformaldehyde to fix it, suck out the excess liquid and add PBS to wash; detection: pour the urine cells on the ...

Embodiment 2

[0031] In embodiment one, add following operation:

[0032] In step 1, the urine sample is the middle-end urine of the patient who urinates for the first time in the morning, and the patient needs to refrain from drinking water for at least eight hours in advance to avoid interference with the test results.

[0033] Remove sample impurities: take a urine sample, pour the urine sample into a test tube, then add an aqueous urease solution to the test tube, and incubate it to remove the urea in the urine sample, then add an active adsorbent, and use ultrasonic vibration to remove Sulfur and phosphorus in urine samples; adding PBS solution: pour the urine sample into a centrifuge for centrifugation, add PBS solution to obtain urine cells resuspended in FPBS; processing vessels: wash the vessels, add APTES solution to In the container, then place the container in the dark for one and a half hours, then suck off the excess APTES, and finally wash it several times with pure water, th...

Embodiment 3

[0035] In embodiment two, add following operation:

[0036] In step 1, the urine sample should be processed within two hours after the urine sample is obtained, or stored in a refrigerator at about 4°C, but not more than 6 hours, so as to avoid the decomposition of substances in the urine sample and affect the detection effect.

[0037] Remove sample impurities: take a urine sample, pour the urine sample into a test tube, then add an aqueous urease solution to the test tube, and incubate it to remove the urea in the urine sample, then add an active adsorbent, and use ultrasonic vibration to remove Sulfur and phosphorus in urine samples; adding PBS solution: pour the urine sample into a centrifuge for centrifugation, add PBS solution to obtain urine cells resuspended in FPBS; processing vessels: wash the vessels, add APTES solution to In the container, then place the container in the dark for one and a half hours, then suck off the excess APTES, and finally wash it several time...

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Abstract

The invention discloses a method for capturing and detecting abnormal metabolic cells in urine. The method comprises the following steps: removing sample impurities; to be specific, taking a urine sample, pouring the urine sample into a test tube, then adding a urease aqueous solution into the test tube, carrying out standing and incubating to remove urea in the urine sample, then adding an activeadsorbent, and removing sulfur and phosphorus in the urine sample by using ultrasonic oscillation; and adding a PBS solution; to be specific, pouring the urine sample into centrifugal equipment for centrifuging, and adding the PBS solution to obtain FPBS resuspended urine cells. According to the method, the technological processes of removing sample impurities, adding a PBS solution, treating a vessel, pouring into the vessel and detecting are set and thus problems that the settling velocity difference is large caused by different viscosities and thus images are not clear and the detection result is inaccurate according to the existing cell capturing and detecting method are solved. The method for capturing and detecting abnormal metabolic cells in urine has advantages of being clear in image and accurate in detection result.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for capturing and detecting abnormal urine metabolic cells. Background technique [0002] A urine test is a medical testing method. Including routine urine analysis, detection of formed components in urine (such as urine red blood cells, white blood cells, etc.), quantitative determination of protein components, urine enzyme determination, etc. Urine examination is of great value in clinical diagnosis, judgment of curative effect and prognosis. [0003] The detection of urine formed components is currently performed by adding urine into a counting tank of a specified volume, and after a certain period of natural settlement, the formed components fall to the bottom of the counting tank, and the various formed components at the bottom of the counting tank are counted by manual observation under a microscope. Or use automatic control means to take images of the bottom o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/34
CPCG01N21/84G01N1/34
Inventor 阎灼辉
Owner HANGZHOU JUNHUI BIOTECHNOLOGY CO LTD
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