Biochar-immobilized naphthalene-degrading microbial agent and preparation method and application thereof

A technology of naphthalene degrading bacteria and biochar, applied in chemical instruments and methods, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., can solve the competition of soil indigenous microorganisms and the easy loss of free strains and other problems, to achieve good repair effect, broad engineering application prospects, and the effect of improving environmental concentration.

Inactive Publication Date: 2020-05-01
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Considering that free strains are easy to lose in practical application and difficult to compete with soil indigenous microorganisms, the development of suitable immobilization technology is the key to ensure that functional microorganisms can efficiently degrade pollutants

Method used

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  • Biochar-immobilized naphthalene-degrading microbial agent and preparation method and application thereof
  • Biochar-immobilized naphthalene-degrading microbial agent and preparation method and application thereof
  • Biochar-immobilized naphthalene-degrading microbial agent and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0035] This embodiment provides a kind of preparation method of biochar-immobilized naphthalene-degrading bacterial agent, and concrete steps are as follows:

[0036] Step 1: Weigh 2g of microcrystalline cellulose and mix it with water in a polytetrafluoroethylene-lined tank, put it into a stainless steel reaction kettle, heat it to 280°C for 2 hours, cool at room temperature after the reaction, and solidify at 12000r / min Then, the solid product was repeatedly washed with distilled water and absolute ethanol, freeze-dried for 12 hours and ground to obtain biochar, which was named MCC280 (see figure 1 );

[0037] Step 2: Mix 0.1g of the frozen naphthalene-degrading strain and 2g of MCC280 biochar into 100mL of LB liquid medium, place in a constant temperature shaker for 12h, the temperature is 37°C, and the shaking rate is 150r / min, to obtain a uniform mixed bacterial solution;

[0038]Step 3: Mix the above-mentioned mixed bacterial solution with sodium alginate solution at a...

Embodiment 2

[0041] This embodiment provides a kind of preparation method of biochar-immobilized naphthalene-degrading bacterial agent, and concrete steps are as follows:

[0042] Step 1: Weigh 5g of microcrystalline cellulose and mix it with water in a polytetrafluoroethylene-lined tank, put it into a stainless steel reaction kettle, heat it to 220°C for 2 hours, cool at room temperature after the reaction, and solidify at 12000r / min Then, the solid product was repeatedly washed with distilled water and absolute ethanol, freeze-dried for 12 hours and ground to obtain biochar, which was named MCC220 (see image 3 );

[0043] Step 2: Mix 0.1g of the frozen naphthalene-degrading strain and 5g of MCC220 biochar into 100mL of LB liquid medium, place in a constant temperature shaker for 12h, the temperature is 37°C, and the shaking rate is 150r / min, to obtain a uniform mixed bacterial solution;

[0044] Step 3: Mix the above-mentioned mixed bacterial solution with sodium alginate solution at ...

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Abstract

The invention discloses a preparation method of a biochar-immobilized naphthalene-degrading microbial agent. The method comprises the steps: weighing biomass raw materials and water, performing mixing, performing heating for a reaction, performing cooling to room temperature after completion of the reaction, performing solid-liquid separation, cleaning the solid product, performing freeze drying,performing grinding so as to obtain biochar, mixing a naphthalene-degrading strain with the obtained biochar in a LB liquid culture medium, putting the obtained mixture in a constant-temperature shaker, performing shaking culture so as to obtain a uniform mixed microbial solution, mixing the mixed microbial solution with a sodium alginate solution, performing magnetic and even stirring so as to obtain a sodium alginate-containing mixed microbial solution, adding the sodium alginate-containing mixed microbial solution dropwise to a calcium chloride solution, putting the mixture in a refrigerator, and performing still standing for a cross-linking reaction so as to obtain the biochar-immobilized naphthalene-degrading microbial agent. The prepared biochar-immobilized naphthalene-degrading microbial agent has an obvious remediation effect on soil which is contaminated by polycyclic aromatic hydrocarbon, and the method has mild reactions, low cost and no secondary pollution, and has broad engineering application prospects.

Description

technical field [0001] The invention relates to a biochar-immobilized naphthalene-degrading bacterial agent and a preparation method and application thereof, belonging to the technical field of microbial remediation of polluted soil. Background technique [0002] Naphthalene is a polycyclic aromatic hydrocarbon substance with 2-ring structure, low toxicity and high efficiency, which is widely used in the production of phthalic anhydride, dye intermediates, rubber additives, pesticides, etc. In October 2017, the International Agency for Research on Cancer of the World Health Organization included naphthalene in the list of 2B carcinogens, and then my country included it in the first batch of priority control chemicals in December 2017. At present, the remediation technologies for PAH-contaminated soil mainly include heat treatment, chemical oxidation, chemical leaching, etc. Although these methods have a significant effect on the removal of PAHs, they are prone to environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N1/20B09C1/10C09K17/16
CPCC12N11/14C12N11/10C12N1/20B09C1/10C09K17/16
Inventor 高品于冬冬张漓杉钟山钱雅洁潘云飞唐正
Owner DONGHUA UNIV
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