Multi-channel tissue scaffold, and preparation method and application thereof

A tissue scaffold and multi-channel technology, applied in biochemical equipment and methods, methods of supporting/immobilizing microorganisms, tissue culture, etc., can solve the problem that organ donation cannot meet the needs of clinical patients, poor mechanical properties of three-dimensional cell culture, and unsuitable Long-term large-scale cultivation and other issues to achieve good biocompatibility, rapid manufacturing, and high through-hole porosity

Inactive Publication Date: 2020-05-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The medical industry is currently facing two difficult problems: organ donation cannot meet the needs of clinical patients; new drug research and development is high investment, high risk but low success rate
However, there are still many problems in the three-dimensional cell culture technology, such as the poor mechanical properties of hydrogel-type three-dimensional cell culture, which is not suitable for long-term large-scale culture, let alone reactor culture

Method used

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  • Multi-channel tissue scaffold, and preparation method and application thereof
  • Multi-channel tissue scaffold, and preparation method and application thereof
  • Multi-channel tissue scaffold, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 Polyglutamic acid multi-channel tissue scaffold

[0074] This embodiment provides a multi-channel tissue support, such as figure 1 As shown, it includes a tissue support body 1, the top of the body 1 has a hollow first channel 2, and the side of the body also has at least one hollow second channel 3, and the first channel 2 is connected to the first channel 2. Two channels 3 are vertical; the body 1 is a porous structure; the material of the body 1 is polyglutamic acid (PGA, the molecular weight is about 150,000). The number of the second channels is 4, parallel to each other. . The diameter (outer diameter) of the multi-channel tissue scaffold described in this embodiment is 1100 μm, and the height is 1000 μm; the diameter of the first channel is about 300 μm, and the diameter of the second channel is about 250 μm. The porosity is 60%.

[0075] The polyglutamic acid (PGA) is tested for cell adhesion, cytotoxicity and mechanical properties, and it is found...

Embodiment 2

[0092] Example 2 polycaprolactone multi-channel tissue scaffold

[0093] Compared with Example 1, the only difference is that the material of the tissue support body is polycaprolactone (PCL).

[0094] Experiments have found that using PCL as the main implementation material also has good biodegradability and biocompatibility, and can be widely used in products such as controlled-release drug carriers, cells, tissue culture scaffolds, and surgical sutures.

[0095] The internal structure of the tissue scaffold prepared in this embodiment is as follows: image 3 shown. image 3 The through holes inside the bracket can be clearly observed in the figure.

Embodiment 3

[0096] Example 3 Poly-L-lactic acid multi-channel tissue scaffold

[0097] This embodiment provides a tissue support, such as figure 1 As shown, it includes a tissue support body 1, the top of the body 1 has a hollow first channel 2, and the side of the body also has at least one hollow second channel 3, and the first channel 2 is connected to the first channel 2. The two channels 3 are vertical; the body 1 is a porous structure; the material of the body is poly-L-lactic acid (PLLA for short). The number of the second channels is 4. The porosity of the body is around 93.5%. The diameter (outer diameter) of the stent is 2000 μm, and the height is 2000 μm; the diameter of the first channel is about 800 μm, and the diameter of the second channel is about 600 μm. The porosity of the tissue scaffold body is 93.5%.

[0098] This embodiment also provides a preparation method for the above-mentioned tissue scaffold, comprising the following steps:

[0099] 1) Build a three-dimens...

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Abstract

The invention relates to a multi-channel tissue scaffold, and a preparation method and an application thereof. The multi-channel tissue scaffold comprises a tissue scaffold body, wherein a first hollow channel is arranged from the top part to the bottom part of the tissue scaffold body; at least one second hollow channel is also arranged in the side surface of the tissue scaffold body; the included angle between the first hollow channel and the second hollow channel is 0-90 degrees; and the tissue scaffold body has a porous structure. The multi-channel tissue scaffold provided by the inventioncan be used for amplifying cells in a large scale, nondestructively collecting the cells and constructing an in-vitro three-dimensional cell culture microenvironment and an in-vitro three-dimensionaltissue, and can be used for cell biology, tissue engineering, in-vitro drug detection, pathology and pharmacology research.

Description

technical field [0001] The invention relates to a multi-channel tissue support that can be used for cell culture, in particular to a tissue support with complex internal flow channels and its preparation method and application. Background technique [0002] The medical industry is currently facing two difficult problems: organ donation cannot meet the needs of clinical patients; new drug research and development is high investment, high risk but low success rate. Therefore, it is an urgent goal of regenerative medicine to construct large-scale tissue and organ models in vitro for clinical transplantation, construction of cell tissue models, and drug screening and evaluation. No matter what kind of research, it is necessary to first achieve long-term mass culture of cells in vitro. However, due to the lack of vascular network mechanism in vitro and the inability to continuously meet the effective delivery of oxygen and nutrients, large-scale cell culture in vitro is still at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/00A61L27/18A61L27/56A61L27/58A61L27/50
CPCC12M25/14C12N5/0062A61L27/18A61L27/56A61L27/58A61L27/50C12N2513/00C12N2533/30C12N2533/40
Inventor 庞媛刘天坤酒井康行孙伟
Owner TSINGHUA UNIV
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