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Technology for increasing efficiency of synthesizing fucosterol from sargassum thunbergii

A technology of fucosterol and Salina, which is applied in the directions of steroids, microorganism-based methods, microorganisms, etc., can solve problems such as low content, achieve high production rate, achieve sustainable development, and improve the effect of resource utilization.

Pending Publication Date: 2020-06-05
HANGZHOU BROTHER BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sargassum contains sterols and polysaccharides, but the content is low. How to improve the content of useful substances by optimizing the culture conditions of Sargassum is a technical problem that we need to solve

Method used

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  • Technology for increasing efficiency of synthesizing fucosterol from sargassum thunbergii
  • Technology for increasing efficiency of synthesizing fucosterol from sargassum thunbergii
  • Technology for increasing efficiency of synthesizing fucosterol from sargassum thunbergii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A process for improving the efficiency of fucosterol synthesis by sargassum, comprising the steps of:

[0037]The Sargassum used in the experiment comes from the coast of Ningbo, Zhejiang Province. Mature male and female algae are collected and cultivated artificially until the seedlings are about 1 cm long. Select healthy sargassum seedlings and inoculate them into a small reaction tank containing sargassum culture solution. The inoculation density is 80 plants / ㎡, the depth of the culture solution in the reaction tank is 30cm, and the culture solution is replaced every 4 days. 24 days; culture conditions: light intensity 3000lux, temperature 22°C, light-to-dark ratio 12:12, air flow 15ml / min·L; Harvest Sargassum algae, dry naturally for 5 days in a ventilated place, and then place in Pulverize with a pulverizer and pass through a 50-mesh sieve to obtain the sargassum algae powder.

[0038] The sargassum culture solution is obtained by improving the formula of the f / 2 ...

Embodiment 2

[0040] A process for improving the efficiency of fucosterol synthesis by sargassum, comprising the steps of:

[0041] The Sargassum used in the experiment comes from the coast of Ningbo, Zhejiang Province. Mature male and female algae are collected and cultivated artificially until the seedlings are about 2cm in length. Select healthy Sargassum seedlings and inoculate them into a small reaction tank containing Sargassum culture solution. The inoculation density is 70 plants / ㎡, the depth of the culture solution in the reaction tank is 40cm, and the culture solution is replaced every 4 days. for 24 days; the culture conditions are: light intensity 3000lux, temperature 23°C, light-dark ratio 14:10, air flow 20ml / min·L; Harvest Sargassum algae, dry naturally for 5 days in a ventilated place, and then place in Pulverize with a pulverizer and pass through a 50-mesh sieve to obtain the sargassum algae powder.

[0042] The sargassum culture solution is obtained by improving the formu...

Embodiment 3

[0047] Effects of various culture factors on the synthesis of fucosterol in Sargassum spp.

[0048] On the basis of Comparative Example 1, the effects of different light intensities on the synthesis of fucosterol by Sargassum sargassum were verified.

[0049] Set the light intensity as: 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, the unit is lux.

[0050] Crude extraction and detection method of fucosterol:

[0051] Add sargassum algae powder to the alcohol extraction tank, the ratio of algae powder to 85% ethanol is 1g:5ml, heat up to 50°C, under the condition of heat preservation, ultrasonic (power 300W, frequency 25kHZ) assists alcohol extraction for 30min, Stop the ultrasonic treatment, and continue the heat preservation alcohol extraction for 9 hours; then vacuum suction filtration, collect the filter residue and filtrate, place the filtrate at 65°C and concentrate under reduced pressure to recover ethanol until it is concentrated to one-fifth of the original ...

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Abstract

The invention belongs to the technical field of biological culture of algae, and discloses a technology for increasing the efficiency of synthesizing fucosterol from sargassum thunbergii. The technology comprises steps as follows: the sargassum thunbergii is inoculated to a sargassum thunbergii culture solution for culture, and algae are collected; and the sargassum thunbergii culture solution isprepared by extra addition of sodium metasilicate nonahydrate and triammonium phosphate on the basis of f / 2 culture medium. Nutrient substances and illumination conditions of a cultivating environmentare regulated and controlled purposefully in the cultivating process of sargassum thunbergii seedlings, and by means of appropriate nutritive salt concentration and proportion, higher fucosterol synthesis rate can be realized.

Description

technical field [0001] The invention belongs to the technical field of algae biological cultivation, and in particular relates to a process for improving the efficiency of fucosterol synthesis by sargassum. Background technique [0002] The market demand of steroids in the field of medicine is second only to antibiotics. The global output value has reached tens of billions of dollars. There are hundreds of steroids in circulation, and the demand will increase in the future. Steroids are important elements of life and have important physiological and medical functions. Steroid drugs were originally extracted from animal adrenal glands, but their practical application is of little significance. Later, some researchers also tried to use small organic molecules as raw materials to explore the total synthesis process through chemical methods. Although it is theoretically feasible, due to the long synthetic route, low yield, poor reactivity, and difficult disposal of by-products,...

Claims

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Application Information

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IPC IPC(8): C12P33/00C07J9/00C12R1/89
CPCC12P33/00C07J9/00
Inventor 刘加林霍萧勇刘加海方朝杰张渊源
Owner HANGZHOU BROTHER BIO TECH CO LTD
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