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Electrochemical luminescence aptamer sensor for detecting ractopamine, preparation method and application method thereof

An aptamer sensor and ractopamine technology, which is applied in chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, can solve difficult on-site detection, time-consuming, and impact on consumer health, etc. problem, to achieve the effect of improving sensitivity and stability and improving selectivity

Active Publication Date: 2020-06-05
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are strict standards for use. Improper use will cause serious side effects, side effects on animal bodies, hormone residue problems, and also affect the health of consumers.
The European Union has already clearly banned the use of hormonal drugs including ractopamine in food animals. The Chinese government attaches great importance to RAC and has explicitly banned the addition of RAC to animal feed. However, there are still many countries in the world that use RAC in animal feed. The use of RAC with relatively low toxicity, due to irregular management and low price of RAC, the phenomenon of incorrect use of RAC still exists, which will increase the probability of RAC transfer to the human body to a certain extent, and aggravate the harm to the human body
In the prior art, the commonly used methods for detecting ractopamine mainly include high performance liquid chromatography, enzyme-linked immunosorbent assay, liquid chromatography, gas chromatography, electrochemical method, etc., but these methods generally have complicated experimental operations and consume a lot of time. Shortcomings such as time length, it is difficult to promote to the market for on-site testing

Method used

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  • Electrochemical luminescence aptamer sensor for detecting ractopamine, preparation method and application method thereof
  • Electrochemical luminescence aptamer sensor for detecting ractopamine, preparation method and application method thereof
  • Electrochemical luminescence aptamer sensor for detecting ractopamine, preparation method and application method thereof

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Embodiment 1

[0049] (1) Assembling an electrochemiluminescence aptasensor for the detection of ractopamine

[0050] (1) Preparation of PTC-PEI and HKUST-1 materials:

[0051] S1. Accurately weigh 0.1009g of PTCDA and dissolve it in an aqueous solution of sodium hydroxide (0.05M), then slowly add HCl (1M) to it, and a precipitate can be observed, which is washed with deionized water to remove excess substances, and the EDC-containing 10mL PTCA solution of NHS was stirred rapidly at 4°C for 5h, and at the same time, 5mL polyethyleneimine (PEI) was added to the vigorous stirring, and stirred for 12h, a red wine-colored solution was obtained, and then the pH was adjusted to 6-7 with hydrochloric acid Between centrifugation and drying, the red powder product PTC-PEI was obtained, which was stored at 4°C;

[0052] S2. Accurately weigh 2.4408g copper nitrate trihydrate, 0.5812g trimesic acid, ultrasonically treat in 5.0012g dimethyl sulfoxide (DMSO) for 30min, then keep it in an electric furnace...

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Abstract

The invention belongs to the field of electrochemical luminescence detection, and particularly relates to an electrochemical luminescence aptamer sensor for detecting ractopamine, a preparation methodand an application method thereof. The electrochemical luminescence aptamer sensor provided by the invention is formed by loading an aptamer on the surface of a glassy carbon electrode modified by anano composite material HKUST-1 / PTC-PEI. According to the invention, the electrostatic interaction of PTC-PEI and HKUST-1 is fully utilized to jointly modify the surface of the glassy carbon electrode, so the flexibility and stability of electrochemical luminescence are obviously improved. The electrochemical luminescence aptamer sensor is obtained by loading the aptamer through the combination effect of the amido bond, so that the target molecule ractopamine can be specifically recognized, and the selectivity of ractopamine detection is improved.

Description

technical field [0001] The invention belongs to the field of electrochemiluminescence detection, and in particular relates to an electrochemiluminescence aptamer sensor for detecting ractopamine, a preparation method and an application method thereof. Background technique [0002] Numerous food safety problems have seriously affected people's lives. Therefore, the monitoring of food safety is very important. Ractopamine (RAC) is a new type of "lean meat extract" that can be used in livestock feed to increase the lean meat percentage of livestock. It is an adrenergic receptor agonist and can be used in medicine. However, there are strict standards for use. Improper use will have serious toxic and side effects, produce side effects on animal bodies, have hormone residue problems, and also affect the health of consumers. The European Union has already clearly banned the use of hormonal drugs including ractopamine in food animals. The Chinese government attaches great importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N21/76
CPCG01N27/308G01N27/3277G01N27/3278G01N21/76
Inventor 周利君单学凌蒋鼎陈智栋
Owner CHANGZHOU UNIV
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