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A method for improving salt-alkali tolerance of corn seedlings and improving the physical and chemical properties of corn seedling rhizosphere soil

A technology of rhizosphere soil and salt-alkali tolerance, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve problems that are unfavorable to sustainable agricultural development, safety disputes, and serious chemical residues to avoid residue and enrichment, improve saline-alkali tolerance, sucrase activity and catalase activity

Active Publication Date: 2021-06-29
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have used chemical regulation to improve the salt-alkali tolerance of corn seedlings. This method has serious chemical residues, which deteriorates the soil environment and is not conducive to the sustainable development of agriculture.
In recent years, with the development of molecular biology, the research on improving the salt-alkali tolerance of maize seedlings through molecular breeding technology has gradually increased, but some molecular breeding methods, such as transgenic technology, are controversial about their safety, and their prospects need to be investigated

Method used

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  • A method for improving salt-alkali tolerance of corn seedlings and improving the physical and chemical properties of corn seedling rhizosphere soil
  • A method for improving salt-alkali tolerance of corn seedlings and improving the physical and chemical properties of corn seedling rhizosphere soil
  • A method for improving salt-alkali tolerance of corn seedlings and improving the physical and chemical properties of corn seedling rhizosphere soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Klebsiella xenobiotics are used to improve the salt-alkali tolerance of corn seedlings and improve the physical and chemical characteristics of corn seedling rhizosphere soil

[0059] 1. Preparation of bacterial suspension

[0060] Inoculate Klebsiella xenophila in LB liquid medium, cultivate at 30°C and 180rpm until a bacterial suspension containing sufficient bacteria is obtained, and inoculate the bacterial suspension at 6000r·min -1 Centrifuge for 15min, discard the supernatant after centrifugation, wash the precipitate with sterile distilled water and resuspend, repeat 3 times and prepare 1×10 8 cfu / ml bacterial suspension, and then diluted to 1×10 according to the experimental needs 2 cfu / ml, 1×10 4 cfu / ml, 1×10 6 cfu / ml, for use.

[0061] Second, corn germination treatment

[0062] Screen Xianyu 335 corn seeds, select corn seeds with the same size and no damage on the surface, soak them in sodium hypochlorite (concentration 10%) for 10 minutes, an...

Embodiment 2

[0078] Example 2. Klebsiella and Bacillus licheniformis in combination are used to improve the salt-alkali tolerance of corn seedlings and improve the physical and chemical properties of corn seedling rhizosphere soil

[0079] 1. Preparation of bacterial suspension

[0080] Inoculate Klebsiella xenophila in LB liquid medium, cultivate at 30°C and 180rpm until a bacterial suspension containing sufficient bacteria is obtained, and inoculate the bacterial suspension at 6000r·min -1 Centrifuge for 15min, discard the supernatant after centrifugation, wash the precipitate with sterile distilled water and resuspend, repeat 3 times and prepare 1×10 6 cfu / ml bacterial suspension.

[0081] Inoculate Bacillus licheniformis in LB liquid medium, cultivate at 30°C and 180rpm until a bacterial suspension containing sufficient bacteria is obtained, and incubate the bacterial suspension at 6000r·min -1 Centrifuge for 15min, discard the supernatant after centrifugation, wash the precipitate w...

Embodiment 3

[0102] Embodiment 3, field experiment

[0103] 1. Experimental location

[0104] Heilongjiang Bayi Agricultural University Experimental Practice Base, the soil of the experimental site is saline-alkali soil (pH8.9, Na + Content 0.891g·kg -1 ).

[0105] 2. Experimental time

[0106] A total of two batches of experiments were carried out.

[0107] The first batch of sowing time: May 25, 2018, harvest time: October 6, 2018.

[0108] The second batch of sowing time: May 20, 2019, harvest time: October 3, 2019.

[0109] 3. Experimental method

[0110] 1. Carry out conventional continuous field operations such as rotary tillage and stubble removal, post-turning harrowing, fertilization, ridging, and suppression on the plot before sowing.

[0111] 2. Operate according to the following groups:

[0112] Control group (CK): Sow Xianyu 335 corn seeds, add 150ml of water to the soil when sowing, and then cover 200g of soil above the sowing place.

[0113] Experimental group (T1):...

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Abstract

The invention discloses a method for improving the salt-alkali tolerance ability of corn seedlings and improving the physical and chemical properties of rhizosphere soil of corn seedlings. The present invention protects the application of Klebsiella in different places of habitats in any of the following (a1)-(a6): (a1) promotes the growth of corn; (a2) promotes the growth of corn under the saline-alkali environment; (a3) ​​improves the corn root (a4) Improve corn rhizosphere soil enzyme activity in saline-alkali environment; (a5) Improve corn saline-alkali tolerance; (a6) Improve corn saline-alkali tolerance by increasing corn rhizosphere soil enzyme activity in saline-alkali environment ability. The invention uses exogenous beneficial microorganisms in the cultivation process of corn seedlings, improves the salt-alkali tolerance of corn seedlings, avoids the residue and enrichment of chemical components, and meets the development needs of green agriculture. In addition, this method has high safety. It is suitable for promotion and transformation, and is of great significance for improving the micro-ecological environment of the root system and promoting the growth of maize seedlings under saline-alkali stress.

Description

technical field [0001] The invention relates to a method for improving the salt-alkali tolerance ability of corn seedlings and improving the physical and chemical characteristics of rhizosphere soil of corn seedlings. Background technique [0002] my country's saline-alkali land area is huge, which seriously limits the development of planting industry. Maize, as a crop sensitive to saline-alkali components, is also deeply affected. How to improve the rhizosphere soil microenvironment of maize, especially maize seedling stage, and improve the saline-alkali tolerance of maize seedlings will be of great significance to the sustainable development of agriculture in saline-alkaline areas. For a long time, people have used chemical regulation methods to improve the salt-alkali tolerance of corn seedlings. This method has serious chemical residues, which deteriorates the soil environment and is not conducive to the sustainable development of agriculture. In recent years, with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/20A01N63/22A01P21/00A01G22/20A01G7/06
CPCA01G7/06A01N63/00A01G22/20
Inventor 杨丽娟杨克军
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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