Cell tissue nucleic acid extraction method used for gene sequencing

Abstract

The invention discloses a cell tissue nucleic acid extraction method used for gene sequencing. The cell tissue nucleic acid extraction method comprises the following steps of: S1: putting animal tissues in a centrifuge tube, adding a STE buffer solution, and mixing by a vortex oscillator to obtain a first solution; S2: adding a sodium laurylsulfonate solution and protease K into the first solution, and evenly mixing in an overturning and centrifugation device to obtain a second solution; S3: cooling the centrifuge tube to the room temperature, firstly adding a mixed solution of Tris-saturatedphenol, chloroform and isoamyl alcohol into the second solution, overturning, evenly shaking, and carrying out centrifugation to obtain a third solution; and S4: transferring supernatant in the thirdsolution to another centrifuge tube, adding icy absolute ethyl alcohol, after round objects are separated out, sucking into the clean centrifuge tube by a pipette, washing by 75% ethyl alcohol for twotimes, and drying to obtain purified nucleic acid. When the overturning and centrifugation device provided by the invention is used, the centrifuge tube can conveniently rotate in a vertical direction and also can rotate in a horizontal direction so as to achieve an effect of the even shaking and the centrifugation of a sample.

Description

technical field [0001] The invention relates to the technical field of nucleic acid purification, in particular to a cell tissue nucleic acid extraction method for gene sequencing. Background technique [0002] DNA and RNA samples for gene sequencing often rely on obtaining purified nucleic acid samples. There are many sources of DNA or RNA, such as tissues, cells, whole blood, plasma, serum, extracts, which can be obtained from organisms (for example, humans, animals, plants, bacteria, viruses, etc.), and the purification of nucleic acids usually requires the separation of DNA or RNA and other components in the sample source such that the polynucleic acid is free of other cellular enzymes, proteins, compounds and / or debris. [0003] One of the earliest techniques for purifying DNA or RNA was to lyse a cell or tissue sample using, for example, proteinase K, add an equal volume of phenol-chloroform solution to the lysate to extract non-nucleic acid material (e.g., proteins, ...

AI Technical Summary

Problems solved by technology

Although phenol-chloroform extraction can obtain high-purity and high-recovery nucleic acid, this method still has disadvantages, such as the need to repeatedly centrifuge and invert the nucleic acid sample during operation, and there is currently no device for two purposes to achieve this. operate

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