A method for detecting variation in the copy number of sheep znf280by gene and its application
A gene copy number and copy number technology, which is applied in the field of molecular genetics detection, can solve the undiscovered problems such as the correlation of ZNF280BY gene copy number variation and reproductive traits, and achieve the effect of low cost, simple and fast operation, and easy operation
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[0032] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments. The examples are only used to explain the present invention, not to limit the protection scope of the present invention.
[0033] The present invention uses real-time quantitative PCR technology to detect and analyze the ZNF280BY gene copy number variation and gene expression of the sheep Y chromosome, mainly including the following steps: using the DDX3Y gene and β-actin gene that have been verified as single copies by sequencing as reference, and using real-time Quantitative PCR was used to identify the copy number and expression level of ZNF280BY gene.
[0034]In order to improve the accuracy of the results, strict experimental operations are carried out according to the requirements of standardized real-time quantitative PCR operations, including: ensuring the extraction of high-quality genomic DNA and RNA, conducting strict target gene primer desig...
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