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Culture medium containing stem cell activator and culture method of mesenchymal stem cells

A technology of stem cells and culture medium, applied in the direction of cell culture active agent, cytokine/lymphokine/interferon, culture process, etc., can solve the problem of affecting the purity and effect of cytokines, and achieve the maintenance of activity and secretion capacity, concentration High, the effect of reducing reagent consumables

Active Publication Date: 2020-07-10
天津百恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the induction medium has always been present in the stem cell factor and has not been removed, which will affect the purity and effect of the cytokine

Method used

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  • Culture medium containing stem cell activator and culture method of mesenchymal stem cells
  • Culture medium containing stem cell activator and culture method of mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Stem cell activator, made by the following method:

[0043] Take 75g of pioglitazone hydrochloride, 50g of vitamin E, add 130g of dimethyl sulfoxide and stir to dissolve, then add 8g of salidroside, 13mg of stromal cell-derived factor, and 170mg of insulin-like growth factor, and add phosphate buffer with pH=7.1 to 1L.

Embodiment 2

[0045] Stem cell activator, made by the following method:

[0046]Take 50 g of pioglitazone hydrochloride, 20 g of vitamin E, add 100 g of dimethyl sulfoxide and stir to dissolve, then add 5 g of salidroside, 11 mg of stromal cell-derived factor, and 140 mg of insulin-like growth factor, and add phosphate buffer with pH=7.0 to 1L.

Embodiment 3

[0048] Stem cell activator, made by the following method:

[0049] Take 100g of pioglitazone hydrochloride, 100g of vitamin E, add 150g of dimethyl sulfoxide and stir to dissolve, then add 10g of salidroside, stromal cell-derived factor-15mg, insulin-like growth factor-1100mg, add phosphate buffer solution with pH=7.2 to 1L .

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Abstract

The invention discloses a culture medium containing a stem cell activator and a culture method of mesenchymal stem cells. The culture method includes: culturing the mesenchymal stem cells by using a cell culture medium until a fusion degree of the mesenchymal stem cells is 80%, and then discarding the cell culture medium; adding the culture medium containing the stem cell activator; culturing; andcleaning; 2) adding a compound electrolyte solution, culturing, and collecting culture supernatant according to a half volume; 3) adding a half-volume compound electrolyte solution, culturing, and collecting culture supernatant according to the half-volume; 4) repeating the step 3) 4-8 times; and 5) filtering, concentrating, purifying and sterilizing the collected culture supernatant to obtain astem cell factor group. The stem cell factor obtained by the method is stable continuously, high in concentration, free of bovine serum components, and free of animal source components and free of allergens. The method is simple and convenient to operate and low in cost. A stock solution of the stem cell factor can be prepared into different stem cell factor preparation products, and is suitable for different indications and different administration methods.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a stem cell activator, a culture medium containing the stem cell activator and a method for producing stem cell factor group by utilizing mesenchymal stem cells using the culture medium. [0002] technical background [0003] Mesenchymal stem cells (MSCs) are a type of stem cells with self-renewal and multilineage differentiation potential. It has biological properties such as hematopoietic support, immune regulation, angiogenesis, and repair of tissue damage. MSC has the characteristics of high differentiation potential, strong proliferation ability, strong homing migration ability, low immunogenicity, convenient material collection, no restrictions on moral and ethical issues, and easy industrial production. Therefore, it is the most promising pluripotent stem cell for clinical application. [0004] The role of mesenchymal stem cells is mainly reflected in two aspects. One is the rep...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12P21/00C07K14/52
CPCC12N5/0662C12P21/00C07K14/52C12N2500/30C12N2500/38C12N2500/34C12N2501/105C12N2501/21
Inventor 梁璐
Owner 天津百恩生物科技有限公司
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