Lamp primer, kit and detection method for rapid detection of apple scab

Abstract

The invention discloses a LAMP primer, a kit and a detection method for rapid detection of apple scab. The LAMP primer consists of a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP, and a pair of loop primers LF and LB. The outer primer F3 is shown in SEQ ID No.1, the outer primer B3 is shown in SEQ ID No.2, the inner primer FIP is shown in SEQ ID No.3, and the inner primer BIP is shown in SEQ ID No.4, The loop primer LF is shown in SEQ ID No.5, and the loop primer LB is shown in SEQ ID No.6. The rapid detection of apple scab can be realized by using the above three pairs of specific LAMP primers to perform LAMP amplification on the sample to be tested. The early diagnosis of apple scab and the detection and identification of pathogenic bacteria are of great significance to the timely and effective prevention and control of apple scab.

Description

technical field

[0001] The invention relates to the technical field of detection methods for apple scab, in particular to a LAMP primer, a kit and a detection method for rapid detection of apple scab. Background technique

[0002] Apple scab, also known as apple scab, caused by Venturiainaequalis, is one of the important diseases in apple producing areas around the world. The disease mainly harms apple leaves and fruits, and can also infect petioles, flowers, sepals, pedicels, young shoots and bud scales, etc. In severe cases, it will cause defoliation, fruit drop, cracking and deformity of the affected fruits, and directly affect the yield and quality of apples. Apple scab has the characteristics of fast epidemic speed, great harm and difficult to control. In recent years, due to the extensive orchard management model, the occurrence of apple scab in the main apple producing areas of my country is on the rise. Apple scab overwintered on bud scales or diseased leaves with ...

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