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A dpx-gc-ms detection method for 2-chlorodiazepam and 4-chlorodiazepam in urine

A DPX-GC-MS and chlordiazepam technology, applied in the field of detection, can solve the problems of different organic solvents, time-consuming and labor-intensive efficiency, and achieve the effect of less organic solvent consumption, high extraction efficiency, and meeting the inspection needs

Active Publication Date: 2022-04-26
无锡微色谱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems that 2-chlordiazepam and 4-chlordiazepam in urine need to use different organic solvents, manual operation, time-consuming and laborious and low efficiency, and provide a urine 2-chlordiazepam -The DPX-GC-MS detection method of chlordiazepam and 4-chlordiazepam

Method used

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  • A dpx-gc-ms detection method for 2-chlorodiazepam and 4-chlorodiazepam in urine
  • A dpx-gc-ms detection method for 2-chlorodiazepam and 4-chlorodiazepam in urine

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specific Embodiment approach 1

[0040] Specific embodiment one: the DPX-GC-MS detection method of 2-chlordiazepam and 4-chlordiazepam in a kind of urine of the present embodiment, it is carried out according to the following steps:

[0041] 1. Extraction of samples to be tested

[0042] 1) Take the urine to be tested in a centrifuge test tube, adjust the pH value to be greater than 9, and set aside;

[0043] 2) After mixing methanol and air, vortex and mix the resin in the tip-type dispersive solid-phase microextraction column, and then discharge; repeat 3 to 6 times;

[0044] 3) After mixing deionized water or buffer solution with air, continue to vortex and mix the resin in the pipe tip dispersion solid-phase microextraction column of step 2), and discharge; repeat 3 to 6 times;

[0045] 4) Use the pipet-tipped dispersive solid-phase microextraction column treated in step 3) to absorb the urine to be tested in step 1), then inhale the air, and vortex to mix the resin in the pipette-tip dispersive solid-ph...

specific Embodiment approach 2

[0069] Specific embodiment two: the difference of specific embodiment one of this embodiment is: in step 2), the pipette tip type dispersion solid-phase microextraction column is connected with the pipette, absorbs the methanol of 0.2~0.8mL, then inhales air, and the pipette tip After the resin in the dispersive solid-phase microextraction column was vortexed and mixed, methanol was discharged; repeat 3 to 6 times. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0070] Specific embodiment three: the difference of the specific embodiment one of this embodiment is: in step 3), draw deionized water or buffer solution through the pipette that is connected with the gun-tip type dispersion solid-phase microextraction column, then inhale air to mix and continue Vortex and mix the resin in the pipe-tip dispersion solid-phase microextraction column in step 2), and discharge the deionized water or buffer solution; repeat 3 to 6 times; the buffer solution is a buffer solution with pH=10. Others are the same as in the first embodiment.

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Abstract

A kind of DPX-GC-MS detection method of 2-clodiazepam and 4-clodiazepam in urine, it relates to the field of forensic science toxicology analysis, the purpose of the present invention is to solve the problem of 2-clodiazepam in urine Pam and 4-chlordiazepam need to use different organic solvents, manual operation, time-consuming, laborious and low efficiency problems, the present invention adopts the pipette type dispersed solid-phase microextraction column that is filled with HLB filler to extract and purify samples, and establishes A GC-MS high-throughput and rapid detection method for 2-clodiazepam and 4-clodiazepam residues in urine. The method can screen and analyze 2-clodiazepam and 4-clodiazepam in urine, meeting the testing needs of actual cases. The invention is applied to the field of forensic science urine detection.

Description

technical field [0001] The invention relates to the detection field, in particular to a DPX-GC-MS detection method for 2-chlordiazepam and 4-chlordiazepam in urine. Background technique [0002] With the increasing control of new psychoactive substances in various countries, criminals often modify the structure of controlled psychoactive substances in order to evade the attack, so that some new psychoactive substances appear. In recent years, some new benzodiazepines have appeared on the illicit drug market. These drugs are similar in structure to traditional benzodiazepines and have similar pharmacological effects, but they have not been approved as drugs by any country. use. These substances are mainly sold in the form of tablets, capsules and powders. [0003] At present, the pretreatment methods of 2-chlordiazepam in urine mainly include direct filtration sampling method and liquid-liquid extraction method, etc., and the main detection methods include gas chromatograph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N2030/062
Inventor 徐仿敏杨佳刘凌云王珏
Owner 无锡微色谱生物科技有限公司
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