Liquid tissue culture device for anoectochilus formosanus sprouts

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a liquid tissue culture device for anoectochilus formosanus sprouts. The device comprises a tissue culture bottle, wherein a first culture bottle body is arranged in the tissue culture bottle; one or more second culture bottle bodies are embedded in the first culture bottle body, and are hollow and bottomless; the bottom ends of the second culture bottle bodies are connected with the bottom end of the first culture bottle body, and the joint parts of the first culture bottle body and the second culture bottle bodies are bottomless. The first culture bottle body and the second culture bottle bodies are arranged, so that the device has the advantages that one tissue culture bottle can be filled with two independent liquid media, quantities of the liquid media in the first and second culture bottle bodies can be respectively controlled; and when the liquid media in the second culture bottle bodies are almost consumed, the effect of drought stress is achieved, and the anoectochilus formosanus sprouts are prompted to root and root out of the second culture bottle bodies to be in contact with the liquid media in the first culture bottle body.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a liquid tissue culture culture device for clematis sprouts. Background technique [0002] The concept of plant tissue culture is also called in vitro culture, which refers to the separation of tissues that meet the needs from the plant body. Organs or cells, protoplasts, etc., are cultured under artificially controlled conditions to obtain regenerated whole plants or produce other products of economic value through aseptic manipulation. The concept of plant tissue culture refers to the cultivation of various parts of plants, such as cambium, parenchyma, mesophyll tissue, endosperm, etc. to obtain regenerated plants, and also refers to the cultivation of callus produced from various organs during the cultivation process, callus Regenerated plants are then regenerated. [0003] In the prior art, there are the following problems. After the differentiation stage of ti...

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Problems solved by technology

[0003] In the prior art, there are the following problems. After the differentiation stage of tissue culture, it is necessary to transfer the sprout explants to carry out the rooting and strong seedling cultivation stage, such as the clematis sprouts in the clematis liquid tissue culture, and the sprouts are transferred to flat Putting it on the support in the liquid medium, the sprouts are easy to soak in water and die during the cultivation process, and the sprouts placed flat will lead to curved upward growth. seedlings, resulting in poor consistency of bottle seedlings, affecting the normal growth of sprouts, and supports cannot be reused and other issues

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