Drugs for resisting novel coronavirus SARS-CoV-2 and application thereof

A coronavirus, sars-cov-2 technology, used in antiviral agents, drug combinations, medical preparations containing active ingredients, etc., can solve problems such as toxic side effects, patient damage, etc., and achieve low toxic side effects and high safety. Effect

Pending Publication Date: 2020-10-02
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above drugs have toxic side effects and may cause unnecessary damage to the patient itself, so finding a less toxic anti-SARS-CoV-2 drug has become an urgently needed solution for the treatment of COVID-19

Method used

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  • Drugs for resisting novel coronavirus SARS-CoV-2 and application thereof
  • Drugs for resisting novel coronavirus SARS-CoV-2 and application thereof
  • Drugs for resisting novel coronavirus SARS-CoV-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024]African green monkey kidney cell Vero was purchased from ATCC; SARS-CoV-2 virus was isolated from the sample; one-step absolute quantitative PCR enzyme Fast Virus 1-Step Master Mix (4444434), Trizol LS was purchased from Thermo; nucleic acid extraction kit Direct- zol TM RNA MiniPrep (R2052) was purchased from Zymo Research; CCK8 kit (40203) was purchased from Shanghai Yisheng Biotechnology Co., Ltd.

[0025] 1. RNA extraction from the supernatant

[0026] Follow the instructions of the nucleic acid extraction kit, as follows:

[0027] 1) Add an equal volume of absolute ethanol to the sample added with Trizol LS, mix well and transfer the liquid to Zymo-Spin TM On the IICR column, the column is placed on the collection tube and centrifuged at 10000g for 1min

[0028] 2) Put the Zymo-Spin TM IICR column transferred to a new collection tube

[0029] 3) On Zymo-Spin TM Add 400 μl of Direct-zol to IICR column TM RNA PreWash, centrifuge at 10000g for 1min, discard ...

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PUM

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Abstract

The invention discloses a series drugs for resisting novel coronavirus SARS-CoV-2 and application thereof. In-vitro cell test results show that compounds disclosed in the invention can significantly inhibit infection of SARS-CoV-2 on normal cells, have an inhibition effect on novel coronavirus SARS-CoV-2 at a cellular level, can significantly reduce the virus titer of novel coronavirus in cells and inhibit proliferation of the novel coronavirus in cells, and has dose dependence. Therefore, the compounds can be used as inhibitors for novel coronavirus SARS-CoV-2, and has the potential of treating COVID-19 pneumonia caused by infection of a human body with the coronavirus SARS-CoV-2 or other diseases caused by infection of animals with the coronavirus SARS-CoV-2.

Description

technical field [0001] The present invention belongs to the field of virology and medicinal chemistry, and in particular relates to a compound that inhibits the replication of the novel coronavirus SARS-CoV-2 virus, the mechanism by which the compound inhibits the replication of the SARS-CoV-2 virus, and the use of the compound in preventing and treating SARS-CoV-2 -Related diseases caused by CoV-2 virus, especially the use on diseases related to inflammation and autoimmunity. Background technique [0002] The novel coronavirus SARS-CoV-2, the Middle East respiratory syndrome coronavirus MERS-CoV, and the severe acute respiratory syndrome coronavirus SARS-CoV belong to the family Coronaviridae. Strand RNA virus, whose genome length is about 26000-32000bp, is the largest RNA virus known so far. [0003] As of May 13, 2020, there were more than 4.2 million confirmed cases of new coronary pneumonia worldwide, and about 290,000 deaths. One of the factors contributing to the hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4422A61K31/343A61K31/5415A61K31/506A61K31/137A61K31/436A61K31/4748A61K31/55A61K31/517A61K31/498A61K31/635A61K31/495A61K31/35A61K31/4412A61K31/4535A61K31/167A61K45/06A61P31/14
CPCA61K45/06A61P31/14A61K45/00A61P11/00
Inventor 王健伟雷晓波肖霞任丽丽王聪慧
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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