Primer pair for detecting Brucella S2 vaccine strain, application thereof, and reagent kit

Abstract

The invention provides a primer pair for detecting a Brucella S2 vaccine strain, an application thereof, and a reagent kit, and belongs to the technical fields of bioengineering and molecular detection. The primer pair consists of a forward primer S2-up and a backward primer S2-down. The primer pair and the reagent kit provided by the invention are used for detection, the detection method is simple and quick, the repeatability is good, the susceptibility is high, the specificity is high, and detection of high-flux samples can be realized, so that quick, high-sensitive real-time fluorescent quantitation PCR detecetion on the Brucella S2 vaccine strain can be realized. A minor quantity of nucleic acid DNA of the Brucella S2 vaccine strain in samples to be detected such as blood can be detected, the detection limit is low, the specificity is high, other bacterial strains rather than the Brucella S2 vaccine strain can be distinguished, and therefore, the primer pair and the reagent kit have favorable practical application value.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and molecular detection, and in particular relates to a primer pair, a kit and an application thereof for detecting Brucella S2 vaccine strain. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Brucellosis can cause brucellosis, referred to as brucellosis, which is a chronic infectious disease that affects humans and animals and is more harmful. It is one of the most widespread zoonotic diseases in the world causing abortions in livestock and disabling infections in humans. Due to increased incidence, it is now considered a re-emergent infectious disease. In Europe, the Americas, Asia, A...

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Problems solved by technology

Molecular biology techniques such as conventional PCR and fluorescent quantitative PCR have been used in the auxiliary diagnosis of brucellosis and the detection of pathogens. Conventional PCR has low sensitivity, while fluorescent quantitative PCR has extremely high sensitivity and good specificity. Affect its application in blood testing

[0006] The use of bacterial vaccines is the main means for humans to fight against bacterial infectious diseases. Although there is currently no effective brucellosis vaccine for human use, a large number of vaccines are used for immunization among animals. The main types are A19, S2 and M5, etc. Live attenuated vaccine, how long does the vaccine strain exist in the animal body after immunization? And when the vaccine strain caused the disease in animals? This is a topic that has not been studied so far, so it is possible to detect whether the blood contains Brucella nucleic acid DNA from a genetic method, thus providing a detection method for the use of vaccines and on-site evaluation of vaccine strains

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