Phytosterol formula for promoting rumen fermentation and determination method thereof

A technology of phytosterol and determination method, which is applied in the field of phytosterol formula and its determination to promote rumen fermentation, can solve the problems of difficulty in judging the influence of phytosterol, different components, different sterol content and ratio, etc., and increase the output of volatile fatty acids , increase gas production, and promote obvious effects

Pending Publication Date: 2020-10-30
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of phytosterol formula that promotes rumen fermentation and assay method thereof, aim to solve the problem that phytosterols in the prior art include β-sitosterol, campest

Method used

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  • Phytosterol formula for promoting rumen fermentation and determination method thereof
  • Phytosterol formula for promoting rumen fermentation and determination method thereof
  • Phytosterol formula for promoting rumen fermentation and determination method thereof

Examples

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no. 1 example

[0029] The invention provides a phytosterol formula for promoting rumen fermentation, which comprises the following raw materials in parts by mass: 0-99.18 parts of total sterols, including: 91.14-99.18 parts of β-sitosterol, 0-29.88 parts of campesterol, 0.10- 95.17 parts stigmasterol.

[0030] Further, the fermentation substrate of the phytosterol formula for promoting rumen fermentation is 600 mg of a complete mixed diet.

[0031] Further, the raw material addition amount of the phytosterol formula for promoting rumen fermentation is 2-12ug per bottle.

[0032] In this embodiment, each sterol sample was fermented outside the rumen liquid in three gradient concentrations, 2 μg, 6 μg, and 12 μg per bottle, and no phytosterol was added as the test control group. The fermentation substrate was 600mg total mixed diet.

[0033] According to the method of Theodorou et al., artificial rumen culture solution was prepared, the rumen solution filtered by four layers of gauze was mix...

no. 2 example

[0035] The invention provides a phytosterol formula for promoting rumen fermentation, which comprises the following raw materials in parts by mass: 0-99.18 parts of total sterols, including: 0-79.71 parts of β-sitosterol, 0-29.88 parts of campesterol, 0.10- 95.17 parts stigmasterol.

[0036] Further, the fermentation substrate of the phytosterol formula for promoting rumen fermentation is 600 mg of a complete mixed diet.

[0037] Further, the raw material addition amount of the phytosterol formula for promoting rumen fermentation is 2-12ug per bottle.

[0038] Further, there are 95.64 parts of total sterols, including: 55.19 parts of β-sitosterol, 29.88 parts of campesterol, and 10.08 parts of stigmasterol.

[0039] In this embodiment, each sterol sample is fermented outside the rumen liquid in 3 gradient concentrations, the 3 concentration gradients are 2 μg, 6 μg, and 12 μg / bottle, and the total sterols are 95.64 parts, of which: 55.19 parts of β-sitosterol, rapeseed oil 2...

no. 3 example

[0042] The invention provides a phytosterol formula for promoting rumen fermentation, which comprises the following raw materials in parts by mass: 0-99.18 parts of total sterols, including: 0-79.71 parts of β-sitosterol, 0-29.88 parts of campesterol, 0.10- 95.17 parts stigmasterol.

[0043] Further, the fermentation substrate of the phytosterol formula for promoting rumen fermentation is 600 mg of a complete mixed diet.

[0044] Further, the raw material addition amount of the phytosterol formula for promoting rumen fermentation is 2-12ug per bottle.

[0045] Further, there are 91.14 parts of total sterols, including: 44.71 parts of β-sitosterol, 27.23 parts of campesterol, and 16.63 parts of stigmasterol.

[0046] In this embodiment, each sterol sample is fermented outside the rumen liquid in 3 gradient concentrations, the 3 concentration gradients are 2 μg, 6 μg, and 12 μg / bottle, and the total sterols are 91.14 parts, including: 44.71 parts of β-sitosterol, rapeseed oil ...

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Abstract

The invention discloses a phytosterol formula for promoting rumen fermentation and a determination method thereof, and phytosterol is added to improve the gas production rate and volatile fatty acid yield of microbial fermentation and reduce the lactic acid yield at the same time. The addition amount of phytosterol has greater influence on rumen fermentation than the sterol component. When phytosterol B2 micrograms is added into each bottle, the promoting effect on rumen fermentation is most obvious.

Description

technical field [0001] The invention relates to the field of phytosterols, in particular to a phytosterol formula for promoting rumen fermentation and a determination method thereof. Background technique [0002] In recent years, with the gradual stabilization of the society and the further improvement of people's living standards, the focus of the breeding industry has gradually shifted from animal growth performance and production efficiency to animal health, feed safety and animal product quality. Phytosterol is a new type of functional green feed additive, which belongs to phytosteroid compound and has plant active ingredients. The phytosterols existing in nature are divided into free type and esterified type. The esterified type phytosterol is more soluble in organic solvents, its absorption and utilization rate is about 5 times higher than that of the free type, and its functions are more extensive. Mingzhu et al. reported that adding a certain amount of phytosterols ...

Claims

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Application Information

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IPC IPC(8): A23K50/10A23K10/30
CPCA23K50/10A23K10/30
Inventor 成艳芬吕东海吴子辰朱伟云朱强
Owner NANJING AGRICULTURAL UNIVERSITY
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