A kind of content determination method of Gastrodia elata 6 index components in Qingda granules

A granule and clear technology, which is applied in the field of determination of the content of six index components of Gastrodia elata, can solve the problems of few reports on the determination of Gastrodia elata content, long time-consuming analysis by HPLC method, and no clear description of content determination, etc. The method is simple and the solvent consumption The effect of small amount and easy operation

Active Publication Date: 2022-08-09
JIANGYIN TIANJIANG PHARMA
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Problems solved by technology

However, the above methods are more suitable for the content determination of gastrodin in Gastrodia elata, and there are few reports on the content determination of Gastrodia elata in compound recipes.
At the same time, the determination of the other five effective chemical components in Gastrodia elata: p-hydroxybenzyl alcohol, barisenoside A, B, C, and E has not been clearly stated whether it is suitable
There are few reports on the relevant determination methods for the simultaneous determination of the six components of Gastrodia elata. In addition, the HPLC method takes a long time to analyze and the operation process is cumbersome.

Method used

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  • A kind of content determination method of Gastrodia elata 6 index components in Qingda granules
  • A kind of content determination method of Gastrodia elata 6 index components in Qingda granules
  • A kind of content determination method of Gastrodia elata 6 index components in Qingda granules

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preparation example Construction

[0065]

[0066] Qingda granules are made from Qingxuan Jiangyatang, and are mainly prepared from the following raw materials by weight: 10-30 parts of Tianma, 10-20 parts of Uncaria, 5-15 parts of lotus seed heart, 5 parts of Scutellaria baicalensis -15 servings. Qingda granules are mainly used for pre-hypertension or grade 1 hypertension. Gastrodia elata is used as the king to calm the liver and yang, supplemented with Uncaria to help clear heat and calm the liver and extinguish wind. It can make the floating yang of the liver hide, and the fire of the heart and liver can be reduced, so as to achieve the hidden yang and calm the liver, suppress the wind, so that the blood pressure can be lowered, and the damage of the heart, brain and kidney can be recovered. Gastrodia elata in the recipe is a traditional Chinese medicine with functions such as relieving wind and relieving spasm, calming liver-yang, etc. It is used for children with convulsions, epilepsy and convulsions. A...

Embodiment 1

[0139] Preparation of the test solution

[0140] Grind the Qingda granules under the item of difference in loading volume, mix well, take about 0.5g, accurately weigh, accurately add 25ml of 0.05% phosphoric acid aqueous solution, weigh to determine the weight, ultrasonically process (power 250W, frequency 40kHz) for 30 minutes, put it in Cool, supplement the lost weight with 0.05% phosphoric acid solution, shake well and centrifuge (3000r / min), accurately measure 10mL of the supernatant, pass it through a polyamide column (80-100 mesh, 2g, inner diameter is 1cm, dry packing column), add 0.05% phosphoric acid for elution, collect the eluate to near the mark, add 0.05% phosphoric acid to the volume to the mark, shake well, and take the filtrate to obtain the test solution.

[0141] Precisely draw 2 μL each of the test solution and the reference solution, inject them into UPLC, and perform the determination according to the aforementioned chromatographic conditions.

[0142] ...

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Abstract

The invention provides a method for determining the content of six index components of Gastrodia elata in Qingda granules. The method improves the preparation method of a test solution. Weigh, add phosphoric acid aqueous solution, weigh, sonicate, let cool, supplement the lost weight with phosphoric acid aqueous solution, centrifuge, take the supernatant to pass through a polyamide column, add elution solvent for elution, and collect the eluate , and then add phosphoric acid aqueous solution to volume, shake well, and take the continuous filtrate. The method is fast and accurate, and can effectively control the quality of Gastrodia elata. It also realizes the qualitative study of Gastrodia elata and Scutellaria baicalensis in Qingda granules, and provides a basis for the development of Qingda granules and other preparations. The invention can reduce the interference of the other three medicinal materials in the Qingda granules to Gastrodia elata, and can greatly reduce other impurity components in the Gastrodia elata, enrich 6 kinds of index components, improve the purity of the index components, and reduce the interference of miscellaneous peaks.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a method for determining the content of six index components of Gastrodia elata in Qingda granules. Background technique [0002] Hypertension is a major risk factor for various cardiovascular diseases, such as stroke, heart failure, atherosclerosis, vascular hypertrophy, etc. Currently, about one-third of adults in my country suffer from hypertension, and the trend continues to rise. According to the 2018 China Guidelines for the Prevention and Treatment of Hypertension, the treatment and control rates of adult hypertension in my country were 46.1% and 16.9%, respectively. Compared with developed countries, it is still at a low level. It can be seen that the prevention and treatment of hypertension is still a key problem to be solved urgently in this research field. [0003] There is no disease name for hypertension in ancient Chinese medicine books, and medical scientists...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8675
Inventor 王协和陈盛君陈可冀李松范恺磊杜俊潮
Owner JIANGYIN TIANJIANG PHARMA
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