Method for determining content of six index components of gastrodia elata in Qingda granules

A granule and clear technology, which is applied in the field of determination of the content of the six index components of Gastrodia elata, can solve the problems of no clear description of the content determination, long time-consuming HPLC analysis, and few reports on the content determination of Gastrodia elata, achieving low solvent consumption, Simple method and good reproducibility

Active Publication Date: 2020-11-13
JIANGYIN TIANJIANG PHARMA
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Problems solved by technology

However, the above methods are more suitable for the content determination of gastrodin in Gastrodia elata, and there are few reports on the content determination of Gastrodia elata in compound recipes.
At the same time, the determination of the other five effective chemical components in Gastrodia elata: p-hydroxybenzyl

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  • Method for determining content of six index components of gastrodia elata in Qingda granules
  • Method for determining content of six index components of gastrodia elata in Qingda granules
  • Method for determining content of six index components of gastrodia elata in Qingda granules

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[0065]

[0066] Qingda granules are made from Qingxuan Jiangyatang, and are mainly prepared from the following raw materials by weight: 10-30 parts of Tianma, 10-20 parts of Uncaria, 5-15 parts of lotus seed heart, 5 parts of Scutellaria baicalensis -15 servings. Qingda granules are mainly used for pre-hypertension or grade 1 hypertension. Gastrodia elata is used as the king to calm the liver and yang, supplemented with Uncaria to help clear heat and calm the liver and extinguish wind. It can make the floating yang of the liver hide, and the fire of the heart and liver can be reduced, so as to achieve the hidden yang and calm the liver, suppress the wind, so that the blood pressure can be lowered, and the damage of the heart, brain and kidney can be recovered. Gastrodia elata in the recipe is a traditional Chinese medicine with functions such as relieving wind and relieving spasm, calming liver-yang, etc. It is used for children with convulsions, epilepsy and convulsions. A...

Example Embodiment

[0138] 1. Example 1

[0139] Preparation of the test solution

[0140] Grind the Qingda granules under the item of difference in loading volume, mix well, take about 0.5g, accurately weigh, accurately add 25ml of 0.05% phosphoric acid aqueous solution, weigh to determine the weight, ultrasonically process (power 250W, frequency 40kHz) for 30 minutes, put it in Cool, supplement the lost weight with 0.05% phosphoric acid solution, shake well and centrifuge (3000r / min), accurately measure 10mL of the supernatant, pass it through a polyamide column (80-100 mesh, 2g, inner diameter is 1cm, dry packing column), add 0.05% phosphoric acid for elution, collect the eluate to near the mark, add 0.05% phosphoric acid to the volume to the mark, shake well, and take the filtrate to obtain the test solution.

[0141] Precisely draw 2 μL each of the test solution and the reference solution, inject them into UPLC, and perform the determination according to the aforementioned chromatographic c...

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Abstract

The invention provides a method for determining contents of six index components of gastrodia elata in Qingda granules. According to the method, a preparation method of a test solution is improved; the preparation method comprises the following steps: taking Qingda granules under a loading amount difference item, grinding, precisely weighing, adding a phosphoric acid aqueous solution, weighing, carrying out ultrasonic treatment, cooling, supplementing the reduced weight with the phosphoric acid aqueous solution, carrying out centrifugal treatment, taking the supernatant, passing through a polyamide column, adding an elution solvent for elution, collecting eluent, adding the phosphoric acid aqueous solution to a constant volume, shaking up, and taking subsequent filtrate. The method is rapid and accurate, can effectively control the quality of gastrodia elata, also realizes qualitative research on gastrodia elata and scutellaria baicalensis in Qingda granules, and provides a basis for development of the Qingda granules and other preparations. Interference of other three medicinal materials in the Qingda granules on gastrodia elata can be reduced, other impurity components in the gastrodia elata can be greatly reduced, six index components are enriched, the purity of the index components is improved, and miscellaneous peak interference is reduced.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and relates to a method for determining the contents of six index components of Gastrodia elata in Qingda granules. Background technique [0002] Hypertension is a major risk factor for various cardiovascular diseases, such as stroke, heart failure, atherosclerosis, vascular hypertrophy, etc. Currently, about one-third of adults in my country suffer from hypertension, and the trend continues to rise. According to the 2018 Chinese guidelines for the prevention and treatment of hypertension, the treatment rate and control rate of hypertension among adults in my country are 46.1% and 16.9%, respectively. Compared with developed countries, it is still at a low level. It can be seen that the prevention and treatment of hypertension is still a key issue to be solved in this research field. [0003] The name of hypertension does not appear in the ancient books of traditional Chinese medicine. Acc...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8675
Inventor 王协和陈盛君陈可冀李松范恺磊杜俊潮
Owner JIANGYIN TIANJIANG PHARMA
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