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Method for determining content of six index components of gastrodia elata in Qingda granules

A granule and clear technology, which is applied in the field of determination of the content of the six index components of Gastrodia elata, can solve the problems of no clear description of the content determination, long time-consuming HPLC analysis, and few reports on the content determination of Gastrodia elata, achieving low solvent consumption, Simple method and good reproducibility

Active Publication Date: 2020-11-13
JIANGYIN TIANJIANG PHARMA
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  • Description
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  • Application Information

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Problems solved by technology

However, the above methods are more suitable for the content determination of gastrodin in Gastrodia elata, and there are few reports on the content determination of Gastrodia elata in compound recipes.
At the same time, the determination of the other five effective chemical components in Gastrodia elata: p-hydroxybenzyl alcohol, barisenoside A, B, C, and E has not been clearly stated whether it is suitable
There are few reports on the relevant determination methods for the simultaneous determination of the six components of Gastrodia elata. In addition, the HPLC method takes a long time to analyze and the operation process is cumbersome.

Method used

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  • Method for determining content of six index components of gastrodia elata in Qingda granules
  • Method for determining content of six index components of gastrodia elata in Qingda granules
  • Method for determining content of six index components of gastrodia elata in Qingda granules

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preparation example Construction

[0065]

[0066] Qingda Granules are produced from Qingxuan Jiangya Decoction, and are mainly prepared from raw materials with the following weight ratio: Gastrodia elata 10-30 parts, Uncaria 10-20 parts, Lianzixin 5-15 parts, Scutellaria baicalensis 5 parts -15 copies. Qingda granules are mainly used for prehypertension or grade 1 hypertension. Gastrodia elata is used as the king to calm the liver and subdue yang, supplemented by Uncaria elata, to help Gastrodia elata clear heat, calm the liver and calm wind, and add Scutellaria baicalensis to clear liver heat, and lotus seed core to vent the heart fire, thereby The floating yang of the liver is hidden, and the heat of the heart and liver is reduced, so as to subdue the yang and calm the liver, restrain the wind, lower the blood pressure, and recover the damage of the heart, brain and kidney. Gastrodia elata in the prescription is a traditional Chinese medicine, which has the functions of relieving wind and spasm, calming li...

Embodiment 1

[0139] Preparation of the test solution

[0140] Take the Qingda granule under the item of difference in loading amount, grind it finely, mix it evenly, take about 0.5g, accurately weigh it, accurately add 25ml of 0.05% phosphoric acid aqueous solution, weigh it, and ultrasonically treat it (power 250W, frequency 40kHz) for 30 minutes, put Cool, use 0.05% phosphoric acid solution to make up the lost weight, shake well and centrifuge (3000r / min), accurately measure 10mL of the supernatant and pass it through a polyamide column (80-100 mesh, 2g, inner diameter 1cm, dry packing column), add 0.05% phosphoric acid for elution, collect the eluate to near the scale line, then add 0.05% phosphoric acid to make it up to the scale line, shake well, and take the subsequent filtrate to obtain the test solution.

[0141] Accurately draw 2 μL each of the test solution and the reference solution, inject them into UPLC, and measure them according to the aforementioned chromatographic conditio...

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Abstract

The invention provides a method for determining contents of six index components of gastrodia elata in Qingda granules. According to the method, a preparation method of a test solution is improved; the preparation method comprises the following steps: taking Qingda granules under a loading amount difference item, grinding, precisely weighing, adding a phosphoric acid aqueous solution, weighing, carrying out ultrasonic treatment, cooling, supplementing the reduced weight with the phosphoric acid aqueous solution, carrying out centrifugal treatment, taking the supernatant, passing through a polyamide column, adding an elution solvent for elution, collecting eluent, adding the phosphoric acid aqueous solution to a constant volume, shaking up, and taking subsequent filtrate. The method is rapid and accurate, can effectively control the quality of gastrodia elata, also realizes qualitative research on gastrodia elata and scutellaria baicalensis in Qingda granules, and provides a basis for development of the Qingda granules and other preparations. Interference of other three medicinal materials in the Qingda granules on gastrodia elata can be reduced, other impurity components in the gastrodia elata can be greatly reduced, six index components are enriched, the purity of the index components is improved, and miscellaneous peak interference is reduced.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and relates to a method for determining the contents of six index components of Gastrodia elata in Qingda granules. Background technique [0002] Hypertension is a major risk factor for various cardiovascular diseases, such as stroke, heart failure, atherosclerosis, vascular hypertrophy, etc. Currently, about one-third of adults in my country suffer from hypertension, and the trend continues to rise. According to the 2018 Chinese guidelines for the prevention and treatment of hypertension, the treatment rate and control rate of hypertension among adults in my country are 46.1% and 16.9%, respectively. Compared with developed countries, it is still at a low level. It can be seen that the prevention and treatment of hypertension is still a key issue to be solved in this research field. [0003] The name of hypertension does not appear in the ancient books of traditional Chinese medicine. Acc...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8675
Inventor 王协和陈盛君陈可冀李松范恺磊杜俊潮
Owner JIANGYIN TIANJIANG PHARMA
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