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Intergeneric distant hybrid creation method of crossostephium chinense and broad-leaved dendranthema vestitum

A technology of distant hybrids and Hibiscus chrysanthemum, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of few applications, difficult to peel off, small immature embryos, etc.

Active Publication Date: 2020-11-17
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Embryo rescue, as an important means to overcome the barriers of distant hybridization, has been applied in Chrysanthemum, but because Hibiscus has no tongue-shaped flowers, immature embryos are small and difficult to peel off, the application of related technologies in Hibiscus is not much

Method used

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  • Intergeneric distant hybrid creation method of crossostephium chinense and broad-leaved dendranthema vestitum
  • Intergeneric distant hybrid creation method of crossostephium chinense and broad-leaved dendranthema vestitum
  • Intergeneric distant hybrid creation method of crossostephium chinense and broad-leaved dendranthema vestitum

Examples

Experimental program
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Effect test

Embodiment 1

[0060] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products. Example 1 Embryo rescue using young embryos of different embryonic ages to obtain intergeneric hybrid offspring of Hibiscus chrysanthemum and Chrysanthemum broadleaf

[0061] Embryo rescue of young embryos of different embryonic ages was used to obtain the intergeneric hybrid offspring of Hibiscus chinensis and Mossia broadleaf. Include the following steps:

[0062] 1. Preparation of parents: the female parent is selected from the diploid salt-tolerant wild species of Hibiscus chrysanthemum, which is a special product in my country, and the male parent is selected from the hexaploid moderately salt-tolerant wild species of Chrysanthemum chr...

Embodiment 2

[0069] Example 2 Using media with different hormone ratios for embryo culture to obtain intergeneric hybrid offspring of Hibiscus chrysanthemum and Chrysanthemum broadleaf

[0070] The intergeneric hybrid progeny of hibiscus hibiscus and mossia broadleaf were obtained by embryo culture in medium with different hormone ratios. Include the following steps:

[0071] 1. Preparation of parents: the female parent is selected from the diploid salt-tolerant wild species Hibiscus chrysanthemum, which is a special product in my country, and the male parent is selected from the hexaploid wild species Maohuahua. Both parents are planted at the Xiaotangshan Base of the National Flower Engineering Center. Adjust the flowering period of Hibiscus chrysanthemum, put it into the artificial climate chamber in August and start 8 hours of short-day curing, so that it will reach the full flowering period in mid-October; the short-day artificial climate chamber is set at 70% humidity, 25°C during th...

Embodiment 3

[0079] Example 3 Utilizing Morphological Observation and Cytology to Identify the Authenticity of Intergenus Hybrids of Hibiscus chrysanthemum and Broadleaf Chrysanthemum chinensis

[0080] Identification of the authenticity of the intergeneric hybrids of Hibiscus hibiscus and Phyllanthus broadleaf by morphological observation and cytology. Include the following steps:

[0081] 1. Identification of Hybrid Authenticity Using Morphology

[0082] 1) The hybrid parents and offspring were planted in the open field in May for routine maintenance and management. Morphological observation and statistics were carried out on hybrids and parents, including: determination of plant height, crown width, and leaf traits.

[0083] 2) Each plant randomly selects three fully expanded leaves, measures the leaf length and leaf width, and measures the length of the petiole respectively; wherein the plant height, crown width, leaf length, leaf width, and petiole length are between the parents. i...

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Abstract

The invention discloses an intergeneric distant hybrid creation method of crossostephium chinense and broad-leaved dendranthema vestitum. The method includes the steps that artificial cross breeding is carried out by taking diploid crossostephium chinense in crossostephium chinense genera of compositae as a female parent and hexaploid broad-leaved dendranthema vestitum in chrysanthemum genera of the compositae as a male parent; F1-generation plants are obtained through in-vitro culture on the immature embryos; and hybrid morphology and cytology identification are carried out on the F1-generation material to finally obtain intergeneric true hybrid progenies of the crossostephium chinense and the broad-leaved dendranthema vestitum. According to the intergeneric distant hybrid creation methodof the crossostephium chinense and the broad-leaved dendranthema vestitum, the intergeneric distant hybridization obstacle of the crossostephium chinense and the broad-leaved dendranthema vestitum isovercome, distant hybrid progenies are obtained, and a batch of new germplasm is created.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for creating intergenus distant hybrids of hibiscus chrysanthemum and broad-leaved chrysanthemum chrysanthemum. Background technique [0002] Hibiscus chrysanthemum (Crossostephium chinense), a single genus and single species of Asteraceae, is unique to my country and is produced in the southeast coastal area. Evergreen subshrub. The leaves are often narrowly oblanceolate, often entire, densely covered with gray pubescence on both sides, and thick in texture; the flower diameter is about 0.5cm, light yellow, without tongue-shaped flowers, and the flowering period is from late November to January of the next year. Hibiscus chrysanthemum is a rare natural plant with different color leaves and aromatic plants. It also has the characteristics of salt and alkali resistance, wind resistance and less pests and diseases, so it is widely used in gardens. Yang Haiyan (2016) used 8 ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/008A01H4/001
Inventor 孙明郭彦宏许婷陈俊通钟剑李大伟巴婷婷常丽娜张启翔程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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