Universal riemerella anatipestifer egg yolk antibody as well as preparation method and application thereof

A technology for Riemerella anatipestiferi and yolk antibodies, applied in the field of general-purpose Riemerella anatipestifer yolk antibodies and its preparation

Active Publication Date: 2020-11-27
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The outbreak of Riemerella anatipestifer is often the result of the joint action of multiple RAs, which poses a great challenge to the control of RA

Method used

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  • Universal riemerella anatipestifer egg yolk antibody as well as preparation method and application thereof
  • Universal riemerella anatipestifer egg yolk antibody as well as preparation method and application thereof
  • Universal riemerella anatipestifer egg yolk antibody as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] 1. Construction of pET-28a-Porin vector

[0034] 1. With the genomic DNA of Riemerella anatipestifer ATCC 11845 as a template, carry out PCR amplification, the amplified product is 1185bp (as shown in SEQ ID NO.2), and its primer sequence is as follows:

[0035] Porin F-BamHI: 5'-GGATCCGTACCAGATACTATTATCGTTC-3' (SEQ ID NO.3),

[0036] Porin R-Not I: 5'-GCGGCCGCAATTCCTATTTCTACTTGAAATCT-3' (SEQ ID NO. 4).

[0037] The reaction system (total volume 20 μL) is:

[0038]

[0039] The amplification reaction conditions are:

[0040]

[0041] Agarose gel electrophoresis, the results of figure 1 shown. Using Tiangen Agar Gel Recovery Kit (DP130419) to recover the PCR products by gel cutting.

[0042] 2. Ligate the recovered PCR product to the pMD-19T simple vector (this embodiment uses a commercially available ligation kit, such as Treasure Biological pMD TM 19-TVectorCloningKit), transform Escherichia coli DH5α, and extract the recombinant plasmid for sequencing. Sp...

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Abstract

The invention provides a universal riemerella anatipestifer egg yolk antibody as well as a preparation method and application thereof. The egg yolk antibody is prepared by steps of taking riemerella anatipestifer recombinant protein as an antigen to immunize laying hens and collecting eggs in an immunization period. The universal riemerella anatipestifer egg yolk antibody overcomes the defects ofcross immunization among serotypes and weak protection effect in riemerella anatipestifer prevention and treatment at present, and can be used for preventing and treating riemerella anatipestifer of various serotypes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a general-purpose Riemerella anatipestifer yolk antibody and a preparation method and application thereof. Background technique [0002] Riemerella anatipestifer (RA), RA is a Gram-negative short bacillus, without flagella and spores, does not move, can produce capsules, and can infect domestic ducks, geese, turkeys, pheasants, etc. At present, the main pathogenic bacteria that endanger the waterfowl breeding industry are widely distributed in duck farms all over the world, and it is difficult to remove them after infection. The infection route of RA is mostly respiratory tract infection and skin wound (especially foot skin) infection, causing an acute or chronic septic bacterial infectious disease. Typical pathological changes are fibrinous pericarditis, perihepatitis, and air sacculitis. The infection rate of the disease can sometimes be as high as 90%, but due to the large d...

Claims

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Application Information

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IPC IPC(8): C07K14/195C07K16/12C07K16/02C12N15/31C12N15/70A61K39/40A61P31/04
CPCC07K14/195C07K16/1203C07K16/02C12N15/70A61P31/04C07K2317/11A61K2039/505A61K2039/552
Inventor 管庆丰杨慧韩谦张海文廖承红赵建国王金花刘又铭
Owner HAINAN UNIVERSITY
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