Method for overcoming self-incompatibility of radish plants

A kind of affinity and radish technology, applied in the field of breeding, can solve the problems that need to be improved, such as plant salinization efficiency, and achieve the effect of low cost, high germination rate and high efficiency

Inactive Publication Date: 2020-12-01
SHANDONG AGRICULTURAL UNIVERSITY +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Method for overcoming self-incompatibility of radish plants
  • Method for overcoming self-incompatibility of radish plants
  • Method for overcoming self-incompatibility of radish plants

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Embodiment 1

[0035] Radish is a plant of the genus Radish with strong self-incompatibility. In breeding research, a small amount of inbred lines can be propagated by pollinating senescent stigmas, but the mechanism of action is unknown. The present invention firstly observes the relationship between senescence and the strength of self-incompatibility. When the radish enters the initial flowering period, mark the radish flowers that open on the same day, and observe the radish flowers that are 0 to 4 days after flowering; Self-pollen was granted by nature, and the growth of pollen tubes and seed setting were observed. like figure 1 It is the result graph of aging radish stigma loss of self-incompatibility, in which, A is the radish flower 0-4 days after flowering; B is the growth of pollen tube of the radish flower 0-4 days after flowering after self-pollination ; C is a quantitative analysis of the number of pollen tubes passing through the stigma of radish flowers 0-4 days after floweri...

Embodiment 2

[0040] Radish is a plant of the genus Radish with strong self-incompatibility. When the radish enters the initial flowering period, in the time period of 8:00~10:00 in the morning on a sunny day, the ethephon solution (0mM as the control group) of 3mM, 5mM, and 8mM mixed with 0.0125% Tween-20 was sprayed on the On the stigma that unfolds into a cross flower, with an interval of 30 minutes in between, spray for the second time, spray twice each time, and fog droplets can be seen on the stigma. The present invention handles and makes the average pollen tube number passing through each radish stigma increase to 54.38,85.35,35.48 by original 0, self-pollen germinates and passes through radish stigma (see for details Figure 4 , spraying ethephon solution mixed with Tween-20 to promote the germination of self-pollen pollen and pass through the radish stigma results, where A is the growth of self-pollination pollen tubes sprayed with 0mM ethephon on the radish stigma, B is the radis...

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Abstract

The invention relates to a method for overcoming self-incompatibility of radish plants, and belongs to the technical field of breeding. The method comprises the following steps: spraying an ethephon solution mixed with Tween-20 on stigmas which are just unfolded to be cross-shaped at the beginning of an initial flowering stage, and completely drying fog drops on the stigmas after spraying and performing pollinating from pollen of plant self. The method disclosed by the invention is low in cost, high in efficiency, convenient to operate and suitable for the self-incompatible line of the radishplants, can overcome the self-incompatibility of other cruciferae crops or other family crops caused by stigma mastoid cell disorders, and has important value for breeding and seed production of the cruciferae crops.

Description

technical field [0001] The invention relates to the technical field of breeding, in particular to a method for overcoming the self-incompatibility of plants of the genus Radish. Background technique [0002] Self-incompatibility is ubiquitous in flowering plants, and it is common in hermaphroditic plants. It is a genetic mechanism formed during the long-term evolution of flowering plants to prevent self-pollination and promote cross-pollination. Self-incompatibility means that self-flowering pollen cannot germinate on its own stigma, or cannot penetrate the stigma after germination, or the pollen tube cannot continue to grow in the style after penetrating the stigma, or the pollen tube reaches the sperm cell after the embryo sac It cannot combine with egg cells, but heterogamy pollen can complete the process of germination, growth, and egg cell combination. Self-incompatibility has important theoretical and practical significance in plant reproductive biology and the utiliz...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G7/06A01N57/20A01P21/00
CPCA01G7/06A01H1/02A01N57/20
Inventor 段巧红黄家保王淑芬原玉香魏小春苏士齐
Owner SHANDONG AGRICULTURAL UNIVERSITY
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