A method for creating new pear self-compatibility germplasm by using pollen-mediated antisense expression of s-rnase

Abstract

The invention discloses an antisense expression method for self-compatibility novel germplasm of an S-RNase creation pear by utilizing pollen mediators and belongs to the molecular genetics field. According to the antisense expression method for the self-compatibility novel germplasm of the S-RNase creation pear by utilizing the pollen mediators, an antisense expression vector pGSA1285-S3 is built through a double-enzyme-digestion method, the antisense expression vector pGSA1285-S3 is transferred to a juicy pear with yellow pear pollen serving as a vector through a method of processing the pollen mediators through ultrasonic waves, three plants containing the antisense expression vector are screened out according to molecular identification of progeny strains, and the novel germplasm of the self-compatibility pear is obtained. The antisense expression method for the self-compatibility novel germplasm of the S-RNase creation pear by utilizing the pollen mediators has the advantages of overcoming a defect that the existing self-compatibility pear is rare in variety resource, providing a new way for cultivating and innovating varieties of the self-compatibility pear, meanwhile providing good testing materials for functional identification of S-RNase gene protein products and having good breeding practical significances and theoretical research values.

Description

technical field [0001] The invention belongs to the field of molecular genetics and relates to a method for creating pear self-compatibility new germplasm by using pollen to mediate antisense expression of S-RNase. Background technique [0002] The pear tree is the third largest fruit tree in my country, and it is also a traditional fruit for foreign exchange earning in my country. It has many varieties and is widely distributed. It is cultivated all over the country except Hainan Province. Pear is a typical gametophytic self-incompatibility fruit tree of Rosaceae. Few of the currently cultivated varieties show self-fertilization. Therefore, pollination trees or artificial pollination must be arranged in production to obtain corresponding yield. From the point of view of species evolution and utilization of heterosis, self-fertilization is beneficial to plants. However, for the fruit tree production for the purpose of harvesting fruit, it is undoubtedly unfavorable that the...

AI Technical Summary

Problems solved by technology

[0004] The self-incompatibility of pear is controlled by multiple alleles at a single locus, including the S gene that controls the self-incompatibility of style and pollen respectively. When the S genotype of pollen and the S allele in style At the same time, the S glycoprotein (S-RNase), the expression product of the S gene, has enzymatic activity, which will degrade the rRNA in the pollen tube, so that the pollen tube cannot be elongated, and pollination and fertilization cannot be completed, so it cannot bear fruit

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