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Application of reagent for increasing NO content of stigmas for improving selective fertilization of cruciferous plants and improvement method

A cruciferous, selective fertilization technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve problems such as incompatibility of distant hybridization, and improve the selective fertilization and application scope of cruciferous plants. Wide and less harmful effect on plants

Pending Publication Date: 2022-01-04
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the purpose of the present invention is to provide the application and improvement method of the agent for improving the NO content of the stigma in improving the selective fertilization of cruciferous plants, significantly improve the NO content of the stigma, and overcome the self-incompatibility and remoteness of the cruciferous plants. The problem of hybrid incompatibility

Method used

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  • Application of reagent for increasing NO content of stigmas for improving selective fertilization of cruciferous plants and improvement method
  • Application of reagent for increasing NO content of stigmas for improving selective fertilization of cruciferous plants and improvement method
  • Application of reagent for increasing NO content of stigmas for improving selective fertilization of cruciferous plants and improvement method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Chinese cabbage removes the flowers that have opened one day before adding GSNO, and collects the seed bags. The next morning, add GSNO solution twice to the newly opened flowers (the GSNO solution contains 0.0125% Tween20, and the GSNO concentration is 0mM, 0.1mM, 0.2mM, 0.5mM), with an interval of 30min, and the second drop After self-pollination, the nesting belts are used to prevent contamination of other pollen. After 12 days, the fruit pods were dissected, and the number of enlarged ovules was counted.

[0048] The result is as Figure 2 to Figure 5 As shown in Table 1, submission of GSNO significantly increased the stigma NO content of Chinese cabbage (for details, see figure 2 ), so that the average number of pollen tubes passing through each cabbage stigma increased from 0 to 2.93, 4.29, 12.93 (see image 3 ); Make the fruit pod length of 12 days after selfing of Chinese cabbage improve respectively from original 9.11mm to 25.20mm, 32.80mm, 32.80mm (for det...

Embodiment 2

[0052] Chinese cabbage removes the flowers that have opened one day before adding GSNO, and collects the seed bags. The next morning, emasculate the newly opened flowers and submit 2 times of GSNO solution (GSNO solution contains 0.0125% Tween20, GSNO concentration is 0mM, 0.1mM, 0.5mM, 1mM), each interval is 30min, and the second drop After the cabbage pollen is given, the seed belts are interlocked to prevent contamination of other pollen. After 12 days, the fruit pods were dissected, and the ovules in the fruit pods were counted.

[0053] The result is as Figure 6 ~ Figure 8 As shown in Table 2, after dropping GSNO, the average number of cabbage pollen tubes passing through each cabbage stigma increased from 0 to 13.75, 91.81 (for details, see Image 6 ); Make the fruit pod length of 12 days after the distant hybridization of Chinese cabbage and cabbage improve from original 0mm to 4.45mm, 24.34mm respectively (for details see Figure 7 ); after the distant hybridizatio...

Embodiment 3

[0057] Chinese cabbage removes the flowers that have opened one day before adding GSNO, and collects the seed bags. The next morning, emasculate the unopened flower buds, add 2 times of GSNO solution dropwise (GSNO solution contains 0.0125% Tween20, GSNO concentration is 0mM, 0.1mM, 0.5mM, 1mM), each interval is 30min, the second time After dripping, the pollen of the European mountain mustard was given, and the seed belt was collected to prevent other pollen from being polluted. After 12 days, the fruit pods were dissected, and the ovule situation in the fruit pods was counted.

[0058] The result is as Figure 9 ~ Figure 11 As shown in Table 3, after adding GSNO dropwise, the number of pollen tubes passing through each cabbage stigma increased from 0 to 0.50, 22.80, 58.70 (see Figure 9 ); Make the fruit pod length of 12 days after the distant hybridization of Chinese cabbage and European mountain thaliana increase to 0.95mm, 2.61mm, 12.72mm respectively from original 0mm ...

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Abstract

The invention provides application of a reagent for increasing the NO content of stigmas for improving selective fertilization of cruciferous plants and an improvement method, and relates to the technical field of crop breeding. The invention provides application of the reagent for improving the NO content of the stigmas for improving the selective fertilization of the cruciferous plants, and further provides the reagent for improving selective fertilization of the cruciferous plants and the improvement method. The reagent or the method is simple and convenient to operate, easy to popularize, remarkable in effect of overcoming self-incompatibility and distant hybridization incompatibility, and small in damage to plants; and moreover, the method is wide in application range, can be applied to overcoming self-incompatibility of the cruciferous crops such as Chinese cabbages, cabbages and radishes and distant hybridization incompatibility with other cruciferous plants, and has important value for breeding and seed production of the cruciferous crops.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and in particular relates to the application of a reagent for increasing the NO content of a stigma in improving selective fertilization of cruciferous plants. Background technique [0002] Self-incompatibility is a genetic mechanism evolved by angiosperms to prevent self-pollination and promote cross-pollination, which plays an important role in preventing self-depression and promoting offspring fitness. According to the source of male recognition factors, self-incompatibility can be divided into sporophytic self-incompatibility and gametophytic self-incompatibility. Cruciferous plants such as Chinese cabbage are typical sporophyte self-incompatibility, showing that self-pollen cannot germinate on the stigma, or entangle on the stigma papillae cells after germination and cannot enter the stigma, while heterogamic pollen can germinate , grow, enter the stigma and complete the fertilization...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02A01H1/021
Inventor 段巧红黄家保崔晓爽杨琳吕金枝
Owner SHANDONG AGRICULTURAL UNIVERSITY
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