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Method for efficiently breeding radish self-incompatible line

A radish and affinity technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of low yield and high cost, achieve artificial regulation, improve reproduction efficiency, and overcome radish self-incompatibility. sexual effect

Active Publication Date: 2021-10-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems existing in the above-mentioned self-incompatibility parent breeding, the present invention provides a method for efficiently propagating radish self-incompatibility lines. The purpose of the present invention is to effectively overcome radish self-incompatibility, simple and efficient Solve the problems of high cost and low yield when breeding radish self-incompatible parents, simplify the propagation and storage of radish self-incompatible materials, and promote the large-scale application of radish hybrid seed production

Method used

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  • Method for efficiently breeding radish self-incompatible line
  • Method for efficiently breeding radish self-incompatible line
  • Method for efficiently breeding radish self-incompatible line

Examples

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Embodiment 1

[0026] Example 1 A method for efficiently propagating radish self-incompatibility lines

[0027] (1) Preparation of melatonin solution

[0028] The concentrations of melatonin solutions were 50, 100, and 200 μM, respectively. The melatonin solution is to dissolve melatonin by using an appropriate amount of absolute ethanol as a cosolvent, and dilute it to 1 L with deionized water to obtain a 500 μM melatonin mother solution, which is stored in a refrigerator at 4° C. in the dark. When used, they were diluted with deionized water to obtain melatonin solutions with final concentrations of 50, 100, and 200 μM, respectively. The prepared solution was put into a 100ml small watering can with a nozzle, and stored in a refrigerator at 4°C in the dark.

[0029] (2) Plant material

[0030] In this example, two radish high-generation self-incompatibility lines 'NAU-YXYB' and 'NAU-WXQ' were selected as test materials, provided by the Radish Genetics and Breeding Laboratory of the Coll...

Embodiment 2

[0042] A method for efficiently propagating radish self-incompatibility lines

[0043] (1) Preparation of melatonin solution

[0044] The concentration of melatonin solution was 100 μM. The melatonin solution is to dissolve melatonin by using an appropriate amount of absolute ethanol as a cosolvent, and dilute it to 1 L with deionized water to obtain a 500 μM melatonin mother solution, which is stored in a refrigerator at 4° C. in the dark. The melatonin solution was diluted with deionized water to a final concentration of 100 μM before use. The prepared solution was put into a 500ml watering can, and stored in a refrigerator at 4°C in the dark.

[0045] (2) Plant material

[0046] In this study, seven radish self-incompatibility lines 'NAU-LV791', 'NAU-YXYB', 'NAU-DF3', 'NAU-WXQ', 'NAU-YH', 'NAU-XZH', 'NAU -YB' is the test material provided by the Laboratory of Radish Genetics and Breeding, College of Horticulture, Nanjing Agricultural University.

[0047] (3) Processing...

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Abstract

The invention discloses a method for efficiently breeding a radish self-incompatible line, and belongs to the technical field of breeding of cruciferous radish crops. The method comprises the following steps: in the full-bloom stage of the radish self-incompatible line, spraying a melatonin solution with a proper concentration on the stigma of a blooming radish flower at 9-11 o'clock in the morning, spraying once every other day until the full-bloom stage is finished, and meanwhile, finishing efficient propagation of the radish self-incompatible line by combining an osmia pollination technology. According to the method, a novel chemical reagent melatonin is sprayed on radish stigmas, so that the breeding efficiency of the radish self-incompatible line is remarkably improved; and meanwhile, the treatment operation is simple and convenient, the cost is low, the problem that the radish self-incompatible line parent is difficult to propagate in a large area can be effectively solved, the application of utilizing the self-incompatible line to carry out efficient seed production of the radish F1 generation is promoted, the practical value is high, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of radish crop breeding, and in particular relates to a method for efficiently propagating radish self-incompatibility lines. Background technique [0002] Radish (Raphanus sativus L., 2n=2x=18), also known as radish and calabash, belongs to the first and second year root vegetable crops of the Brassicaceae radish. Radish is a typical cross-pollinated plant, most of which have self-incompatibility (Self-Incompatibility, SI), and the heterosis is very obvious. Using self-incompatibility lines to prepare hybrids is one of the important ways to utilize heterosis. Strong advantages, easy breeding, short cycle and other advantages. When preparing hybrids, using highly self-incompatible lines as parents can increase the hybridization rate and hybrid purity, but due to the existence of radish self-incompatibility, it will be difficult for the parents to self-seed and set seeds, and it is difficult to complete larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/00
CPCA01H1/02A01H1/129Y02A40/25
Inventor 王燕王娟倪萌王琴王爽柳李旺徐良
Owner NANJING AGRICULTURAL UNIVERSITY
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