Multi-nano transmission system and preparation method thereof

Abstract

The invention discloses a multi-nano transmission system which is obtained by dissolving host molecules and guest molecules in water according to an equal molar ratio, fully stirring for reaction, dialyzing and freeze-drying, wherein the guest molecules are obtained by coupling DSPE-PEG-NHS with amantadine hydrochloride under an alkaline condition. The preparation method of the host molecules comprises the following steps: firstly, taking beta-cyclodextrin as an initial raw material, carrying out ring opening on glycidol under the alkaline condition to obtain beta-cyclodextrin grafted hyperbranched polyglycerol beta-CD-HPG; then taking dicarbonyl imidazole as a coupling reagent, and linking tris (2-aminoethyl) amine to hydroxyl of beta-CD-HPG so as to obtain beta-cyclodextrin grafted hyperbranched polyglycerol grafted tris (2-aminoethyl) amine beta-CD-HPG-TAEA; and then carrying out targeted modification on the beta-CD-HPG-TAEA by using a targeted reagent so as to obtain the host molecules. The multi-nano transmission system can load small molecular drugs and nucleic acid fragments at the same time, and the loaded small molecular drugs and nucleic acid fragments can be flexibly adjusted according to use requirements.

Description

technical field

[0001] The invention relates to a nano drug carrier, in particular to a multiple nano delivery system and a preparation method thereof. Background technique

[0002] The nano-drug delivery system based on amphiphilic polymers can overcome the low bioavailability, poor stability and pharmacological effects of existing pharmaceutical preparations by combining their advantages such as long circulation, targeting, controlled release, transmucosal, transdermal and physicochemical responses. Short time, serious adverse reactions and other defects have attracted extensive attention of researchers in recent years.

[0003] Tumor tissue structure is complex and proliferates rapidly, resulting in heterogeneity of tumor tissue structure and complex tumor microenvironment. Single drug therapy has limited effect, and long-term use will also cause drug resistance problems. The combination therapy of drugs and genes is a new and effective method for the treatment of tumors...

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