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Qinghai plateau indigenous morchella strain separation technology

A technology for bacterial species separation and indigenous sheep, applied in horticulture, botanical equipment and methods, plant cultivation and other directions, can solve problems such as affecting the yield of Morchella, unstable yield, and difficult management.

Pending Publication Date: 2020-12-04
青海卓辰农业科技发展有限公司
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Problems solved by technology

Morchella traditionally mainly relies on collecting wild species. In recent years, the area of ​​artificial cultivation has been expanding, but the cultivation technology is not perfect, especially the strain separation technology, the success rate is less than 10%. Affected by the environment, climate and other conditions, it only grows once a year, and it is difficult to collect, so the output is very small and the price is quite expensive
[0006] The existing Qinghai plateau morel cultivation mainly faces three major problems: one is the strain degradation, which affects the output of morel; Thirdly, the introduced Morchella fungus has a high infection rate, which makes Morchella hyphae lose their growth advantage compared with miscellaneous fungus hyphae and cannot be effectively purified.
This technical shortcoming in the separation of local morel strains in Qinghai has increased the risk and cost of morel planting, making the separation technology of morel strains a major bottleneck in the development of the morel industry in Qinghai

Method used

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  • Qinghai plateau indigenous morchella strain separation technology

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preparation example Construction

[0019] The preparation method of the mother seed medium is as follows: add 1100 ml of distilled water to the pot, cut 200 grams of fresh peeled potatoes into small pieces and add them to the water, boil on low heat for 15 minutes and then filter. Set the volume to 1000 ml, add agar, glucose, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1, stir well until completely melted, and obtain the mother seed culture medium, which can be put into test tubes, and each test tube is filled with 10 ml of mother seed culture. Seal it with a cotton plug or a silicone plug, put it into a sterilizer, keep it at 120°C for 30 minutes, take it out, and put it on an inclined plane under sterile conditions to solidify for later use.

[0020] Step 3 Select seed mushrooms: select wild morels with robust fruiting bodies, no deformities, no pests and diseases, and a maturity of 6-8, pull them out together with the substrate soil, and separate and cultivate them within 2 hours after pick...

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Abstract

According to the technical scheme, a Qinghai plateau indigenous morchella strain separation technology comprises the specific separation steps of preparing a culture medium, selecting seed mushrooms,separating mother strains, culturing and purifying hyphae and the like. Wild morchella esculenta in Qinghai is collected, a morchella esculenta variety which is most suitable for Qinghai alpine regions and is high in yield, high in stability and optimal in quality is screened out through many years of separation, purification and cultivation research, an optimal cultivation technology is summarized in combination with local special weather conditions, and yield per mu of cultivated morchella esculenta reaches 150 kg or above. The produced morchella esculenta is strong in mushroom flavor, largein size, thick in texture and richer in nutrition.

Description

[0001] 1. Technical field [0002] The invention relates to a separation technology of morel strains native to the Qinghai plateau, and belongs to a separation method of morel strains. [0003] 2. Background technology [0004] Morel (Morchella deliciosa Fr.) is a rare and valuable edible and medicinal fungus, which is the king of fungi. It has the functions of strengthening the body, preventing and curing diseases, prolonging life and improving human immunity. It is not only a treasure on the banquet, but also a well-known good medicine in medicine. It used to be a nourishing tribute to the royal family. Morchella traditionally mainly relies on collecting wild species. In recent years, the area of ​​artificial cultivation has been expanding, but the cultivation technology is not perfect, especially the strain separation technology, the success rate is less than 10%. Influenced by the environment, climate and other conditions, it only grows once a year, and it is difficult to...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 管俊林
Owner 青海卓辰农业科技发展有限公司
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