Qinghai plateau indigenous morchella strain separation technology
A technology for bacterial species separation and indigenous sheep, applied in horticulture, botanical equipment and methods, plant cultivation and other directions, can solve problems such as affecting the yield of Morchella, unstable yield, and difficult management.
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[0019] The preparation method of the mother seed medium is as follows: add 1100 ml of distilled water to the pot, cut 200 grams of fresh peeled potatoes into small pieces and add them to the water, boil on low heat for 15 minutes and then filter. Set the volume to 1000 ml, add agar, glucose, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1, stir well until completely melted, and obtain the mother seed culture medium, which can be put into test tubes, and each test tube is filled with 10 ml of mother seed culture. Seal it with a cotton plug or a silicone plug, put it into a sterilizer, keep it at 120°C for 30 minutes, take it out, and put it on an inclined plane under sterile conditions to solidify for later use.
[0020] Step 3 Select seed mushrooms: select wild morels with robust fruiting bodies, no deformities, no pests and diseases, and a maturity of 6-8, pull them out together with the substrate soil, and separate and cultivate them within 2 hours after pick...
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