Biological analysis method based on quantum dot selective recognition reaction and application thereof

A biological analysis and selective technology, applied in the field of biomedical diagnostic analysis, can solve the problems of limited analytical sensitivity of analytical methods, and achieve the effect of improving analytical sensitivity

Active Publication Date: 2020-12-18
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a biological analysis method and its application based on the selective recognit

Method used

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  • Biological analysis method based on quantum dot selective recognition reaction and application thereof
  • Biological analysis method based on quantum dot selective recognition reaction and application thereof
  • Biological analysis method based on quantum dot selective recognition reaction and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This example provides the synthesis method of CdTe QDs.

[0030] The CdTe QDs of this example were synthesized by a one-pot method, as follows:

[0031] First, 0.5mmol CdCl 2 and 0.20 g of trisodium citrate were dissolved in 50 ml of water, and 52 μL of mercaptopropionic acid (MPA) was added to the above solution. Using NaOH solution, the pH of the above mixture solution was adjusted to 10.5. Then, 0.1mmolNa 2 TeO 3 and 50mg KBH 4 Add it to the above solution, and reflux for 1 hour until the solution turns red, showing strong red fluorescence under the irradiation of ultraviolet light. Finally, the CdTe QDs solution was purified by precipitation (using n-propanol) and centrifugation (11000 rpm, 30 min). The MPA-CdTe QDs synthesized above were stored at 4 °C before use.

Embodiment 2

[0033] This example provides the preparation methods and selective cation exchange reaction conditions for different DNA template CuNPs. details as follows:

[0034] Shake 70 microliters of 10mmol / L 3-(N-morpholine) propanesulfonic acid (MOPS) buffer solution, 20 microliters of DNA strands (poly T chain, dsDNA) with different concentrations and mix well, add 40mmol / L 5 microliters of ascorbic acid ; shake for 30 seconds; then add 5 microliters of 2mmol / L Cu 2+ , oscillating for 30 seconds, and standing for 3 minutes at room temperature to react; finally, use a molecular fluorescence spectrophotometer to detect the fluorescence signal.

[0035] Add CdTe QDs to the Cu NPs solution of different DNA strand templates above, and let it stand for 3 minutes at room temperature. Subsequently, the fluorescence signal changes were detected using a molecular fluorescence spectrophotometer.

[0036] In order to elaborate on the effect of QDs on Cu 2+ and the selective recognition pheno...

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Abstract

The invention relates to a biological analysis method based on quantum dot selective recognition reaction and application thereof. The method comprises the steps of selectively recognizing Cu <2+> anda DNA template Cu NPs based on QDs to obtain the change of a QDs fluorescence signal, and obtaining the analysis result of a target object based on a nucleic acid chain based on the change of the QDsfluorescence signal. Therefore, the selective cation exchange reaction based on the QDs is introduced through the DNA template Cu NPs, the DNA template Cu NPs-QDs can be used as signal molecules, andthe analysis sensitivity can be effectively improved in the biological analysis and detection process. The method can be widely applied to the field of detection and analysis of nucleic acid strand-based targets.

Description

technical field [0001] The invention relates to the technical field of biomedical diagnostic analysis methods, in particular to a biological analysis method based on quantum dot selective recognition reactions and its application. Background technique [0002] In the field of biomedical diagnosis and biochemical analysis, in terms of operation steps, there are mainly two types: heterogeneous and homogeneous. Among heterogeneous methods, enzyme-linked immunosorbent assay (ELISA) is the most typical, but it requires labeling and separation steps. In the homogeneous analysis method, the entire detection process is in a centrifuge tube, only the reaction solution is added to the centrifuge tube, and finally diluted and tested on the instrument, which does not require steps such as separation and washing. Although there are a variety of homogeneous fluorescence analysis strategies in the prior art, their analysis sensitivity is limited, which can only meet the needs of general d...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486G01N2021/6432
Inventor 陈飘飘白云金应斌武
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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