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Carbomer/lysozyme hydrogel and application thereof in wound healing

A technology of hydrogel and lysozyme, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, enzymes, etc., can solve the problems of limiting the use of lysozyme

Pending Publication Date: 2020-12-25
泰州学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above deficiencies limit the use of lysozyme in burn patients

Method used

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  • Carbomer/lysozyme hydrogel and application thereof in wound healing
  • Carbomer/lysozyme hydrogel and application thereof in wound healing
  • Carbomer/lysozyme hydrogel and application thereof in wound healing

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Embodiment Construction

[0032] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0033] 1. Experimental method

[0034] 1. Preparation method and identification

[0035] 1.1 Preparation of carbomer / lysozyme hydrogel

[0036] Weigh 3.0g Carbomer 940 and dissolve it in 80mL sterile water, let it fully swell overnight. Use sodium hydroxide with a concentration of 1mol / L to adjust the pH to neutral, then sterilize under high pressure (103-115kPa) at 121°C for 20min; accurately weigh 0.01g of lysozyme (egg white lysozyme, purchased from sigma) and dissolve it in 10mL Sterile water, then add it to the previously prepared carbomer hydrogel, and finally add sterile water to 100mL, mix well.

[0037] 1.2 Quality identification

[0038] (1) Gel identification reaction

[0039] Identification reaction 1: Accurately weigh 1.0 g of carbomer / lysozyme hydrogel w...

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Abstract

The invention discloses carbomer / lysozyme hydrogel and application thereof in wound healing. The hydrogel is prepared by the following method comprising the steps of: S1, weighing a proper amount of carbomer 940, dissolving the carbomer 940 in sterile water, and fully swelling the carbomer 940 overnight; S2, adjusting the pH value to be neutral, and performing high-temperature and high-pressure sterilization to obtain carbomer hydrogel; and S3, precisely weighing a proper amount of lysozyme, dissolving the lysozyme in sterile water, adding the lysozyme into the prepared carbomer hydrogel, finally adding a proper amount of sterile water, and performing uniform mixing to obtain the lysozyme hydrogel. The hydrogel meets preparation requirements and has a slow-release effect; furthermore, thecarbomer 940 in the hydrogel can improve the stability of the lysozyme and reduce the hemolytic effect of the lysozyme, so that the safety is improved; and therefore, the carbomer / lysozyme hydrogel can be used for promoting wound healing of burn patients.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a carbomer / lysozyme hydrogel and its application in wound healing. Background technique [0002] Lysozyme (lysozyme) is a natural antibacterial protein that widely exists in various secretions of mammals and poultry egg whites. It has the characteristics of strong heat resistance, strong resistance to organic solvents, and not easy to produce drug resistance. The antibacterial effect of lysozyme is mainly through the destruction of the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the bacterial cell wall, so that the insoluble mucopolysaccharide of the cell wall is decomposed into soluble glycopeptide, resulting in cell wall Rupture, allowing the contents to escape and lyse the bacteria. At the same time, because lysozyme can break the β-1,4 glycosidic bond of chitin in the fungal cell wall, so lysozyme can dissolve the fungal cell wall an...

Claims

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Application Information

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IPC IPC(8): A61K38/47A61K9/06A61K47/32A61P17/02
CPCA61K38/47A61K9/06A61K47/32A61K9/0014C12Y302/01017A61P17/02
Inventor 张鑫张欢韦平和高新星彭加平
Owner 泰州学院
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