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Marker for examining pancreatic ductal adenocarcinoma by using methylation level of locus CpG and application of marker

A technology of pancreatic ductal adenocarcinoma and methylation markers, which is applied in the field of molecular biomedicine, can solve the problems of increasing patient trauma, and achieve the effect of non-invasive detection process, high safety, and improved convenience

Pending Publication Date: 2021-01-05
中国人民解放军海军军医大学第一附属医院 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For patients with typical imaging findings but negative EUS-FNA biopsy, re-EUS-FNA biopsy is often required, which further increases the trauma to the patient

Method used

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  • Marker for examining pancreatic ductal adenocarcinoma by using methylation level of locus CpG and application of marker
  • Marker for examining pancreatic ductal adenocarcinoma by using methylation level of locus CpG and application of marker
  • Marker for examining pancreatic ductal adenocarcinoma by using methylation level of locus CpG and application of marker

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Experimental program
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Embodiment 1

[0200] Using the aforementioned method of using the kit and the analysis method of the sequencing results, using the 10 probes in Table 1 that can be used to diagnose the PDAC signal level in plasma samples, the probe numbers are: seq_01, seq_02, seq_03, seq_04, seq_05, seq_06 , seq_07, seq_08, seq_09, seq_10, and their actual measurement and analysis results in clinical samples. The clinical samples were derived from plasma samples provided by Changhai Hospital, and the clinical samples included samples from PDAC positive and healthy individuals, and the number of samples was 165.

[0201] Wherein, the clinical samples are divided into detection training group samples (sample 1-123, 123 cases) and test group samples (sample 124-165, 42 cases); the training group samples are used for model building; the test group samples are used for Verify the established model Table 2: The number, sequence and genome coordinates of the 10 probes.

[0202] Such as figure 2 As shown, using...

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Abstract

The invention provides a marker for examining pancreatic ductal adenocarcinoma by using a methylation level of a locus CpG and application of the marker. The cfDNA methylation marker provided by the invention can be used for effectively identifying the pancreatic ductal adenocarcinoma; the PDAC methylation marker protected by the patent is remarkably different in level in cfDNA of normal people and ctDNA of PDAC sufferers and thus can be used for judging whether an examined object suffers from the risk of the PDAC or not. The marker can be used for accurately examining actual levels of PDAC methylation markers corresponding to all panels in a sample provided by an examinee; and by employing reagents, panels, adapters and library building flows provided by a kit, a high-richness and high-diversity DNA methylation library can be prepared from a small amount of cfDNA or ctDNA samples, and signal levels of the PDAC methylation markers provided by the patent are assayed accurately and are used for evaluating whether the examined object suffers from the PDAC or not.

Description

technical field [0001] The invention belongs to the technical field of molecular biomedicine, and in particular relates to a marker for detecting pancreatic ductal adenocarcinoma by using the methylation level of a CpG site and an application thereof. Background technique [0002] Pancreatic ductal adenocarcinoma (PDAC) is one of the malignant tumors with the worst prognosis in solid tumors, with a 5-year overall survival rate of less than 5%. Early diagnosis and surgical resection is the only possible cure for PDAC. In my country's economically developed regions, the estimated number of new PDAC cases ranks seventh among males and sixth among females, and shows a rapid upward trend. [0003] Early PDAC has an insidious onset, rapid progression, high resistance to chemotherapy, and high mortality. Reliable and accurate early diagnosis and treatment methods are urgently needed for early detection. Patients with advanced PDAC usually need to have a clear pathological diagnos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11G16B20/20
CPCC12Q1/6886G16B20/20C12Q2600/154
Inventor 金钢郭世伟刘蕊邹文斌何其晔
Owner 中国人民解放军海军军医大学第一附属医院
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