Stem cell induction culture solution as well as preparation method and application thereof

A technology for stem cell culture and induction medium, which is applied in the field of stem cell induction culture medium and its preparation, can solve the problems of low induction differentiation rate and long time, and achieve the effects of ensuring cell activity, fast induction speed, and improving centrifugation effect

Active Publication Date: 2021-01-12
威海见生生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional culture method induces a low differentiation rate and takes a long time

Method used

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  • Stem cell induction culture solution as well as preparation method and application thereof
  • Stem cell induction culture solution as well as preparation method and application thereof
  • Stem cell induction culture solution as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: The present invention provides a technical solution: a stem cell induction culture solution, the stem cell culture solution includes an inducer, an induction medium and a pH buffer, the content of the inducer is 0.4-1.4 μg / ml, The content of the induction medium is 0.6-2.8 μg / ml, and the content of the pH buffer solution is 0.03-0.5 μg / ml. .

[0029] In this embodiment, the inducer is sulfonated β-cyclodextrin or oleic acid, and the induction medium contains any two or more of fetal bovine serum, amino acids, and penicillin.

[0030] In this embodiment, the pH buffer is sterile phosphate buffer, and its pH value is 7.2-7.5.

[0031] In this embodiment, the stem cell culture medium is obtained by separating and culturing tissue cells, and the tissue cells include tissue cells of mammals including humans.

[0032] The preparation method and application of the above-mentioned stem cell induction culture medium include the following steps:

[0033] S1: Afte...

Embodiment 2

[0037] Example 2: see Figure 1-3 , in this embodiment, the centrifugal device in S2 includes a casing 1, a first centrifugal casing 2 placed in the casing 1, a second centrifugal casing 3, and a centrifugal casing 2 installed in the first centrifugal casing 2, a second centrifugal casing 3 The centrifugal structure on the bottom side of the centrifugal housing 3, the first centrifugal housing 2 and the second centrifugal housing 3 have the same structure and are symmetrically distributed, and the inside of the box body 1 is provided with a driving first centrifugal housing 2 and a second centrifugal housing. 3 running drive assembly; the drive assembly includes a first motor 4 and two rotating rods 5, one end of the two rotating rods 5 is affixed to the top of the first centrifugal housing 2 and the second centrifugal housing 3, and the other end of the rotating shaft is connected to the top of the second centrifugal housing 3. The top of the box body 1 is connected by rotati...

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Abstract

The invention belongs to the field of cell experiments, and particularly discloses a stem cell induction culture solution and a preparation method and application thereof. The stem cell induction culture solution comprises an inducer, an induction culture medium and a PH buffer solution, wherein the content of the inducer is 0.4-1.4 [mu]g / ml, the content of the induction culture medium is 0.6-2.8[mu]g / ml, the content of the PH buffer solution is 0.03-0.5 [mu]g / ml, the inducer is sulfonated beta-cyclodextrin or oleic acid, and the induction culture medium contains any two or more than two of fetal calf serum, amino acid and penicillin. The induction culture solution is high in induction speed and high in induction rate, can be used for inducing adipose cells with different sizes, has goodcompatibility, and is beneficial to maintaining the difference of cell functions while ensuring the cell activity.

Description

technical field [0001] The invention relates to the field of cell experiments, in particular to a stem cell induction culture medium and its preparation method and application. Background technique [0002] Cell culture technology refers to the growth of cells under in vitro conditions in which the cells no longer form tissues (animals). As a method of cultivating cells, cell culture technology has been deeply and widely used in the field of biology to observe the morphological structure and life activities of living cells and various disciplines such as cytology, genetics, immunology, experimental medicine and oncology. Research is of great significance to both clinical and basic research. [0003] The rise of stem cell regenerative medicine research provides a new therapeutic approach for the repair of organ damage. The basic method is to culture and expand autologous normal tissue cells in vitro. However, the traditional culture method induces a low differentiation rate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2509/10
Inventor 李炳根袁牧赵晓彤
Owner 威海见生生物技术有限公司
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