Method for rapidly determining multi-component mycotoxin in beans and bean products

Abstract

The invention discloses a method for rapidly determining multi-component mycotoxin in beans and bean products. The method comprises the following steps: respectively preparing an extracting agent, a purifying agent A and a purifying agent B according to the proportion, treating the sample by using the extracting agent, the purifying agent A and the purifying agent B, and carrying out qualitative and quantitative analysis on the multi-component mycotoxin by using UPLC-MS/MS. The extracting agent and the purifying agent are prepared from chemical agents with low laboratory cost, the ratio of theextracting agent to the purifying agent is optimized, the prepared extracting agent and purifying agent can better purify a complex sample matrix so as to remove sample analysis interference components, so that the measurement accuracy and precision are improved; the method is short in operation time and high in extraction and purification effect; and quantitative analysis of multi-component mycotoxins such as AFTB1, OTA, T-2, HT-2, ST, FB1, ZEN, NIV, DON, 3-AcDON and 15-AcDON can be completed within 15 min in combination with the UPLC-MS/MS determination technology, the determination efficiency is high, quantification is sensitive, and errors are small.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for rapidly determining multi-component mycotoxins in beans and bean products. Background technique [0002] Mycotoxins are toxic substances produced by the metabolism of toxin-producing fungi in a suitable environment. Mycotoxins can contaminate plant-derived agricultural products such as grains, beans, fruits, and vegetables, and thus cause human health and livestock and poultry breeding safety through food chain enrichment. Serious threat, mycotoxin contamination is regarded as the culprit of foodborne diseases by WHO and FAO. [0003] In recent years, due to the rapid development of mycotoxin sample analysis technology, mycotoxin sample analysis methods (UPLC method, HPLC-pre-column / post-column derivatization method, ELISA method, TLC method, colloidal gold quantitative detection method, etc. , immunoaffinity column-fluorescence photometry, etc.), sampl...

AI Technical Summary

Problems solved by technology

The existing national standard analysis methods for mycotoxins are mainly for the analysis of grains, fruits, vegetables and feed, while beans and their products are often due to the particularity of the sample matrix: for example, soybeans often contain a large amount of protein and complexity: for example, fermented bean curd There are often dozens of spices added in the drug, and the above analysis methods: UPLC method, HPLC-pre-column / post-column derivatization method, ELISA method, TLC method, colloidal gold quantitative detection method, immunoaffinity column-fluorophotometry cannot Satisfies the multi-component analysis of mycotoxins in beans and their products, and these methods have their own shortcomings in the multi-component analysis of mycotoxins: for example, due to the limitation of detectors in UPLC and HPLC, when analyzing some mycotoxins, it is necessary to Derivatization treatment increases the analysis cost and time; while ELISA method, colloidal gold quantitative detection method and immunoaffinity column-fluorescence photometry are limited by their antigen-antibody specificity, they can only detect single or several fungi Toxin components are analyzed, and multiple components of mycotoxins cannot be analyzed simultaneously, and special analysis kits are required for analysis, which is costly and poor in quantitative accuracy

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