Application of carboxylic acid type cation exchange fibers and fabrics thereof in adsorbing and filtering influenza viruses
A cation exchange and influenza virus technology, applied in the application field of functional fibers, can solve the problems of inability to realize industrial preparation and practical application, expensive starting materials, harsh preparation conditions of functional fibers, etc.
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[0017]Unless otherwise specified, the experimental methods used in the examples are all conventional methods. Unless otherwise specified, the materials and reagents used in the examples can be obtained from commercial sources.
[0018]Reagent
[0019]PBS buffer (10mM, pH 7.4): NaH2PO4 0.24g / L, Na2HPO4 1.42g / L, KCl 0.2g / L and NaCl 8.0g / L, the solvent is water.
[0020]Strain information of influenza virus
[0021]Strain A / PR8 / 8 / 34 (H1N1): referred to as PR8, literature: C-T. Guo et al. Glycobiology 2007, 17, 713-724.
[0022]Acquisition of influenza virus
[0023]Prepare 9-10 day-old SPF chicken embryos, and mark the embryonic chicken air chambers with an egg finder. Use a thin iron wire or a needle to make a hole in the air chamber and at the same time make a hole in the middle of the chicken embryo where there is no blood vessel. Inoculate an appropriate amount of influenza virus seed into 9-10 day old chicken embryos, and seal the punched area with wax. Cultivate in a 37°C incubator, observe the va...
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