409 results about "Avian influenzavirus" patented technology

This invention relates to a monoclonal antibody capable of combining with H5 subtype avian influenza virus HA protein specifically, the hybridoma cell line secreting said antibody and a preparing method. The invention also relates to a serial test kit for testing H5 subtype avian influenza virus by the antibody and a bit of said antibody in the test sample of the virus and its usage in treatment.

The invention provides a method for detecting fowl influenza virus and parting primer system and augmenting fowl influenza virus RNA asymmetrically. The invention designs and screens 25 pairs multiple asymmetrical RT-PCR primers, a pair general primer, 52 specific probes and 3 quality control probes according to the report fowl influenza virus total hypotype genome sequence in Genebank, wherein every pair in 25 pairs multiple asymmetrical RT-PCR primers is fit for a hypotype of fowl influenza virus, the primer system comprising 25 pairs multiple asymmetrical RT-PCR primers and a pair general primer can be used for detecting and parting fowl influenza virus, the primer system builds the method of asymmetrically augmenting fowl influenza virus RNA and detecting and parting fowl influenza virus. The invention provides strong specificity, high sensibility, which also proceeds the first step application.

Antimicrobial compositions having a rapid and persistent antiviral effectiveness against influenza viruses, including avian flu viruses, are disclosed. The antimicrobial compositions contain (a) a disinfecting alcohol, (b) an organic acid, and (c) water, wherein the composition has a pH of about 5 or less and the nonvolatile components of the composition are capable of forming a barrier film or layer on a treated surface.

The present invention relates to an artificially synthesized gene optiHA containing codons for chicken partial tropism. Its reading frame contains 1707 bp nucleotides and encodes a total of 568 amino acids. The gene is compatible with H5 subtype highly pathogenic avian influenza virus A/ Goose/GuangDong/1/96(H5N1)[GD/1/96(H5N1)]hemagglutinin (HA) gene has a nucleotide homology rate of 70%, an amino acid homology rate of 100%, and encodes the H5 subtype Hemagglutinin (HA) protein of avian influenza virus GD/1/96 (H5N1). The invention also relates to the application of the gene as an immunogenic gene of H5 subtype influenza DNA vaccine and other genetic engineering vaccines.

The invention discloses a preparation method of an avian influenza virus suitable for growing in a serum-free full-suspended cultured MDCK cell line and the avian influenza virus obtained through the method. The preparation method comprises the following steps of 1 preparation of the MDCK cells to be inoculated; 2 virus seed preparation, wherein a chick embryo source avian influenza virus is prepared; 3 F1 generation virus domestication; 4 F2 generation virus domestication, wherein a supernatant sample retained at the time point when the blood clotting titer of the avian influenza virus obtained in the F1 generation is highest is taken, and the step 3 is repeated; 5 F3 generation virus domestication, wherein the F3 generation virus culturing temperature is 35 DEG C; 6 F4 generation-F10 generation virus domestication, wherein the step 5 is repeated, and the domesticated avian influenza virus is obtained. According to the preparation method, through a domestication method, the avian influenza virus is directly domesticated to completely adapt to be efficiently reproduced on the serum-free full-suspended cultured MDCK cells from the mode of being cultured by a chick embryo, the domestication efficiency is high, the avian influenza virus can be efficiently infected and copied in the MDCK cells, and the virus characteristic is stable.

The invention relates to an anhydrous peramivir crystal compound and a preparation method thereof, and further discloses a pharmaceutical composition comprising the same and the application thereof in the preparation of medicaments for treating the influenza virus and the Avian influenza virus.

The invention discloses an influenza/human and avian influenza virus detection gene chip and a preparation method and an application thereof, 102 probes used for detecting A type, B type, H1N1 hipotype, H3N2 hipotype, H5N1 hipotype and H9N2 hipotype influenza viruses and 4 highly pathogenic differential diagnosis probes, as well as a process of sample treatment, hybridization, elution and chip identification which can carry out detection, typing or identification over the 6 types of viruses simultaneously and can identify the high pathogenicity of viruses. The invention not only greatly shortens the detection time of influenza viruses, but also improves the detection accuracy and provides a feasible technique support for the early warning mechanism of influenza epidemic situation in fields of clinical diagnosis, inspection quarantine, and the like.

The present invention relates to the field of animal virology, and provides a recombinant chicken-origin H9N2 avian influenza virus vaccine strain and a method for isolation, identification and purification of the strain. The invention further relates to a research of biological characteristics of the strain, especially to a research of characteristics of the strain adopted as the vaccine strain,and an evaluation of immune effects of the strain on SPF chickens. The preservation number of the strain is CCTCCNO:V201030. According to the present invention, the antigen variation conditions of the virus strain and other isolated virus strains are represented from the molecular level; after the virus strain is prepared into the vaccine, the prepared vaccine is adopted to immunize the 4 week old SPF chickens, with the protection effect analysis of the homologous H9 influenza wild virus strain and the heterologous H9 influenza wild virus strain, the results show that the influenza virus strain can be adopted as the spare vaccine strain of H9 subtype avian influenza. With the present invention, the spare vaccine strain is provided for prevention of the avian influenza outbreak by using the vaccine, the molecular biology technology program is provided for screen of the avian influenza virus vaccine strain, the molecular biology background is provided for study of the mechanism of animal infection by the avian influenza, and the important public health significance is provided.

The invention discloses a detection kit and detection method for H1, H3 and H9 type avian influenza viruses and belongs to the technical field of biology. The kit comprises an RNA extraction kit, an RNA reverse transcription kit and an RPA detection kit. The RNA extraction kit is used for extracting RNA of a to-be-detected sample to be used as a detection template. The RNA reverse transcription kit is used for conducting reverse transcription on the RNA in the detection template to obtain cDNA. The RPA detection kit comprises primers and fluorescent dye. The primers are used for amplifying the cDNA to obtain an amplified DNA sequence. The primers comprise the forward primer and the reverse primer. The fluorescent dye is used for conducting fluorescence detection on the amplified DNA sequence. According to the detection kit and the detection method, the avian influenza viruses can be rapidly, easily and conveniently detected.

The invention relates to a novel gene sequence of a recombinant chicken Alpha interferon, constructs a recombinant expression vector thereof and belongs to a gene engineering biological product obtained by a molecular biology method. The gene sequence of a newly designed chicken Alpha interferon is recombined into a pPICZ Alpha-A vector and then is confirmed on the special position of a microzyme by an electric conversion mode. Besides, a pichia expression system is adopted to express a foreign gene, thus being beneficial to the commercial production of the chicken interferon. The protein expressed by a gene group after being diluted by 4365158.3 times can completely restrain the attraction of vesicular stomatitis virus of 100-1000TCID50. Compared with a natural chicken Alpha interferon, the novel gene sequence of a recombinant chicken Alpha interferon has a higher anti-virus effect; the anti-virus effect thereof is improved by 8 times. Test also detects that the protein expressed by the gene can restrain the proliferation of a newcastle disease virus and an avian influenza virus; besides, the effect of the interferon with high concentration is more remarkable.

The present invention relates to gene chip for the detection and subtyping of bird flu virus. The present invention establishes gene chip with high specificity, high sensitivity and great practicability for detection and subtyping of bird flu virus through designing and screening 50 pairs of specific primers, 2 universal primers, 52 specific probes and 3 quality control probes for the multiple RT-PCR subtyping detection of bird flu virus genes based on the gene sequences of different bird flu virus subtypes Genebank reports; and performing the effectiveness test, specificity test, sensitivity test and condition optimization of gene chip detection. The present invention establishes also the detection and subtyping process with the chip and gives the initial application.

The invention discloses a triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus and a preparation method and application thereof. The preparation method of the egg yolk antibody comprises the following steps of: (1) selecting a hen; (2) performing triple inactivation immunization of the hen obtained by the step (1) by use of newcastle disease virus, infectious bronchitis virus and avian influenza virus; (3) taking the egg laid by the hen and separating the egg yolk liquid; and (4) separating and purifying an egg yolk antibody from the egg yolk liquid obtained by the step (3). The triple egg yolk antibody disclosed by the invention is of great value in treating and preventing newcastle disease, avian influenza and infectious bronchitis.

The invention relates to a recombinant newcastle pestilence LaSota HCLV expressing the bird flu virus H9 subtype hemagglutinin (HA) protein, in particular the recombinant newcastle pestilence LaSota is rL-H9HA. The invention further discloses a method for preparing the recombinant newcastle pestilence LaSota HCLV and the application of the HCLV in preparing a bivalent vaccine for preventing bird flu and newcastle pestilence.

The invention provides an antigen-antibody complex for preventing and/or treating avian influenza, which comprises inactivation avian influenza totiviruses which are used as antigen and an immune body thereof, and the mass concentration ratio of the antigen and the immune body is more than 1. After entering organisms to perform initial immunization, the antigen-antibody complex stimulates the organisms again to induce immunoreaction, and immune response is quick in speed and strong in reaction; the antigen-antibody complex used as a carrier is more favorable for capturing and presenting antigen presenting cells and can also strengthen a breeder reaction of T cells effectively; purified totiviruses used as the antigen increase the molecular weight of the complex, are more favorable for ingestion of immunocyte of the organisms on the antigen, cause the more extensive immunoreaction quickly, and have a significance for preventing avian influenza viruses of which the antigen is easy for variation. A preparation of the antigen-antibody complex does not need to use solid carriers, does not cause intense stimulus on the organisms or initiates the organisms to generate an adverse reaction, and is simple to prepare and safe and convenient to use.

The invention discloses a virus detector, which comprises an interdigital array microelectrode (1), a micro fluidic detection pool (2) and an impedance detection module (3); when a sample containing virus is injected into the micro fluidic detection pool (2), an antibody or other biological identification materials fixed on the surface of the interdigital array microelectrode (1) embedded in the micro fluidic detection pool (2) can be combined with virus, impedance change is generated, impedance measure is carried out through an impedance detection module (3), data processing is carried out by using a standard curve, so that bird flu virus content can be rapidly and quantitatively determined. The virus detector has the advantages of fast detection speed, convenient operation, quantitative analysis and on-site detection.

The invention discloses a method for silencing an IFNARI gene in a DF-1 cell line. The method includes the steps that according to the IFNARI gene, multiple pieces of siRNA of an RNA interference target spot sequence are designed, and the siRNA knocking down IFNARI gene expression at the mRNA level is screened out; a lentiviral vector containing an IFNARI RNAi gene is built; by means of the lentiviral vector, the IFNARI RNAi gene is mediated into the DF-1 cell line to silence IFNARI gene expression, and the proliferation titer of an avian influenza virus in the DF-1 cell line is improved. The method can increase the antigen amount of the avian influenza virus in the DF-1 cell line by 5-10 times, the production cost of a vaccine is greatly reduced, the problems that the vaccine is insufficient in antigen amount and high in production cost can be solved, and the research and development process of an avian influenza bioreactor cell culture technology is promoted.

The invention discloses a surface enhanced Raman spectra substrate for detecting an avian influenza virus and application of the surface enhanced Raman spectra substrate. The application provided by the invention specifically relates to the application of nano-gold particles coupled with alpha (2 and 3) sialyloligosaccharide in the surface enhanced Raman spectra substrate for detecting the avian influenza virus. Experiments show that the substrate can specifically capture the avian influenza virus in a sample to be detected; the enrichment of avian influenza viruses in the sample can be realized through centrifugal collection, and then the enriched avian influenza viruses are used for SERS (Surface Enhanced Raman Scattering) spectra detection. An interference wave crest caused by sample impurities is effectively removed because of the enrichment of the viruses, and a spectral signal can also be effectively amplified by the nano-gold particles, so that the substrate can be used for the SERS spectra detection of the avian influenza virus. The substrate has a great significance to the rapid detection and identification of the avian influenza virus.

The invention relates to a method which is capable of efficiently converting a cell into a biological probe and applying the biological probe into the detection for virus and bacteria. The method comprises the steps of successfully applying the original method with 'space-time coupling' new strategy put forward by the laboratory into staphylococcus aureus to enable the interior of the bacteria to be lightened by luminescent quantum dots, and ingeniously establishing a signal report body; due to the natural expression protein A on the surfaces of the cells, carrying out incubation on the fluorescent bacteria and antibody without complex metabolism and genetic engineering cell modification, and carrying out centrifugal washing on excessive antibody to enable the surface of cell wall to be combined with the antibody to obtain the probe with targeting property. The method can be used for capturing the virus or bacteria to be detected by an immune magnetic ball by being combined with an immune magnetic trapping method; and the avian influenza H9N2 virus and the rat cold salmonella are successfully detected by the fluorescent targeted probe. The whole method for establishing the cell probe is simple and ingenious, and the detection process is high in specificity and sensitivity.

This invention discloses a latex agglutination kit to quickly detect H5 subtype of avian influenza virus. The anti monoclonal antibodies of H5 subtype of avian influenza virus hemagglutinin protein were coupled to the surface of carboxyl polystyrene latex particles using water-soluble carbodiimide, the methods about detection of H5 subtype of avian influenza virus were established, the latex agglutination detection kits of H5 subtype of avian influenza virus were prepared supplemented by other matching reagents. This kit was made up of box body and the body latex diagnostic reagents in the box, sample handling liquid A, sample handling liquid B, the positive control samples and negative control samples. This invention kit can directly detect the H5 subtype avian flu virus, with high specificity, high sensitivity, simple and rapid diagnosis, and other significant advantages.

The present invention relates to a chicken-origin H9N2 avian influenza virus strain and an application thereof. The avian H9N2 influenza A virus AIV-SD strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 22, 2011. The preservation number of the strain is CGMCC No.4689. The AIV-SD strain of the present invention provides pathogenicity for the chicken, and provides low toxicity for mammalians. After the virus solution of the AIV-SD strain is inactivated, the resulting inactivated AIV-SD strain can be used as the vaccine. After vaccinating with thevaccine of the present invention, the prevention ability of the chickens to the avian influenza can be substantially increased so as to substantially improve the economic benefits of the cultivation industry. The chicken-origin H9N2 avian influenza virus strain of the present invention provides great values for prevention and control of the avian influenza virus.

The invention provides a newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and a preparation method of the bivalent inactivated vaccine, which relate to the field of biological engineering. The bivalent inactivated vaccine comprises a newcastle disease virus LaSota strain and an avian influenza virus A/chicken/HeNan/03/2009/(H9N2) strain. The preparation method of the vaccine comprises the steps of preparing, concentrating and deactivating virus liquid, performing water phase preparation and oil phase preparation, and emulsify, thereby obtaining the bivalent inactivated vaccine. The newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine has good immunogenicity, creates a high antibody level, and can withstand not only the attack of homologous viruses, but also the attack of JX02 and SH01 of H9N2 subtype avian influenza viruses, which are prevalent since 2011; the bivalent inactivated vaccine can create high level antibodies and has a long persistent period; and the preparation method of the bivalent inactivated vaccine is simple and safe.

The invention discloses LAMP (loop-mediated isothermal amplification) primer combination for detecting six respiratory viruses, and the application thereof. The primer combination provided by the invention consists of 36 DNA molecules as shown in the sequence 1 to the sequence 36. The primer combination can be used for detecting whether a to-be-detected virus is influenza A virus subtype H1N1, influenza A virus subtype H3N2, avian influenza virus subtype H5N1, avian influenza virus subtype H7N9, influenza B virus or human adenovirus, and can be used for detecting whether a to-be-detected sample contains influenza A virus subtype H1N1 and/or influenza A virus subtype H3N2 and/or avian influenza virus subtype H5N1 and/or avian influenza virus subtype H7N9 and/or influenza B virus and/or human adenovirus. The primer combination provided by the invention is used for detecting six respiratory viruses, has high specificity and high sensitivity, and can realize simple, convenient, rapid and accurate detection. A great promotional value is achieved.