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Gene engineering antibody of human source neutrality for anti virus H5N1 of bird flu

A technology of genetically engineered antibodies and bird flu viruses, applied in genetic engineering, antiviral agents, antibodies, etc., can solve the problems of no human bird flu vaccine, no therapeutic drugs, and the prevalence of mutated strains

Inactive Publication Date: 2007-12-26
中国疾病预防控制中心病毒病预防控制所
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AI Technical Summary

Problems solved by technology

The HA antigen variation caused by HA gene mutation often leads to immune failure and the prevalence of mutant strains. Influenza viruses frequently and continuously undergo antigenic variation. Currently, there is no effective human avian influenza vaccine or effective treatment drug

Method used

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  • Gene engineering antibody of human source neutrality for anti virus H5N1 of bird flu
  • Gene engineering antibody of human source neutrality for anti virus H5N1 of bird flu
  • Gene engineering antibody of human source neutrality for anti virus H5N1 of bird flu

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Embodiment Construction

[0029] The following preferred embodiments illustrate the present invention in detail, but are not meant to limit the content of the present invention.

[0030] Using phage surface display technology, lymphocytes were obtained from the convalescent blood of an avian influenza patient in Anhui, China, and a human-derived anti-H5N1 avian influenza virus genetically engineered antibody library was constructed by means of genetic engineering. The purified and inactivated human H5N1 Anhui isolate (A / Anhui / 1 / 2005) was used for enrichment screening of the Fab phage antibody library and secreted expression in E.coli. The functional activity of the Fab antibody specifically binding to the H5N1 avian influenza virus was identified by ELISA and indirect immunofluorescence assay (IFA), and the sequence was determined. Then, the positively cloned light chain and heavy chain Fd segment genes were respectively cloned into the whole antibody expression vector pAC-L-Fc to transfect insect Sf9 ...

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Abstract

This invention provides a human-derived genetic engineering antibody that can specifically bond avian influenza virus H5N1. The genetic engineering antibody is derived from antibody gene library of avian influenza virus H5N1 patients in convalescence. The recombinant antibody is decided by hypervariable region (CDRs) specific gene sequence in the antibody light chain and heavy chain gene variable region, and is effectively expressed in procaryotic cells and eukaryotic cells. The recombinant antibody is a functional neutralizing antibody can specifically bond avian influenza virus haemagglutinin protein HA. Part or all genes of the antibody in CDR can modify and produce genetic engineering antibodies with different forms in procaryotic cells, yeast, insects and eukaryotic cells, and can prevent and treat diseases related to avian influenza virus H5N1.

Description

technical field [0001] The present invention relates to the preparation and application of human genetic engineering antibodies for prevention and treatment, especially for the neutralization of highly pathogenic avian influenza virus H5N1, which has the function of neutralizing the infection of avian influenza virus in my country Anti-avian influenza virus genetically engineered antibody. Background technique [0002] Highly pathogenic avian influenza (HPAI) is a severe infectious disease of poultry caused by influenza A virus of the Orthomyxoviridae family. The highly pathogenic avian influenza virus H5N1 has been highly concerned by the international community since 1997 when 18 people were infected and 6 died. Especially since 2003, the highly pathogenic avian influenza virus H5N1 animal epidemic has become endemic in Asia. popular and has spread to Europe and Africa. The number of human infection cases and the countries and regions where human infection cases have occu...

Claims

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Application Information

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IPC IPC(8): C07K16/18C12N15/13A61K39/395A61P31/12
Inventor 梁米芳孙丽娜李德新舒跃龙刘琴芝李川张全福
Owner 中国疾病预防控制中心病毒病预防控制所
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