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Virus detector

A detector and virus technology, applied in the field of virus detectors, can solve the problems of low accuracy, long detection time, low sensitivity, etc., and achieve the effect of semi-automatic operation and fast detection speed

Active Publication Date: 2014-11-26
CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

Virus isolation method is currently the gold standard method for diagnosis and identification, with high sensitivity, but the detection time is too long and takes 7-10 days; RT-PCR is the detection method recommended by the World Health Organization, which takes 3-6 hours and has high sensitivity. However, complex sample pretreatment is required; the test strip detection time is short, usually within 15 minutes, but the sensitivity is low and the false positive rate is high
Existing virus detection methods have shortcomings such as too long time, complicated operation or low accuracy, which to a certain extent restricts grassroots animal disease prevention and control institutions from carrying out rapid screening of animal diseases

Method used

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Embodiment Construction

[0034] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but should not be used to limit the scope of the present invention.

[0035] figure 1 It is a structural schematic diagram of a virus detector in a preferred embodiment of the present invention; the detector includes an interdigital array microelectrode 1 , a microfluidic detection cell 2 and an impedance detection module 3 . This embodiment is used to detect avian influenza virus. The interdigital array microelectrode 1 is used to detect changes in electrochemical impedance, and the microfluidic detection cell 2 is used to precisely control the amount of sample and redox probes flowing through the surface of the interdigital array microelectrode 1. The impedance detection Module 3 is used to accurately measure the impedance amplitude and phase angle changes of the electrodes...

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Abstract

The invention discloses a virus detector, which comprises an interdigital array microelectrode (1), a micro fluidic detection pool (2) and an impedance detection module (3); when a sample containing virus is injected into the micro fluidic detection pool (2), an antibody or other biological identification materials fixed on the surface of the interdigital array microelectrode (1) embedded in the micro fluidic detection pool (2) can be combined with virus, impedance change is generated, impedance measure is carried out through an impedance detection module (3), data processing is carried out by using a standard curve, so that bird flu virus content can be rapidly and quantitatively determined. The virus detector has the advantages of fast detection speed, convenient operation, quantitative analysis and on-site detection.

Description

technical field [0001] The invention relates to the technical field of virus detection, and more specifically to a virus detector. Background technique [0002] In recent years, outbreaks of viral animal diseases have continued to occur, such as avian influenza H5N1, H7N9, etc. According to WHO statistics: Since 2003, animals in 62 countries and people in 15 countries have been infected with the H5N1 bird flu virus. Among the 649 infected cases, 385 died, and the mortality rate was nearly 60%. Highly pathogenic avian influenza has not only caused huge economic losses, but also seriously threatened social public health security. [0003] The key to the prevention and control of viral animal diseases is rapid detection of disease viruses. Taking avian influenza virus as an example, existing methods for detecting avian influenza virus mainly include virus isolation, reverse transcription polymerase chain reaction (RT-PCR) and colloidal gold immunochromatographic test strips. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/02G01N33/569
Inventor 林建涵汪懋华温新华王小红
Owner CHINA AGRI UNIV
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