Sliding type microfluidic organ chip and preparation method thereof

Abstract

The invention provides a sliding type micro-fluidic organ chip and a preparation method thereof. The sliding type micro-fluidic organ chip comprises a sliding assembly and a micro-fluidic chip base, wherein the sliding assembly is provided with a sliding rod, a glue injection opening, a sealing plug and an embedded tissue cavity, the glue injection opening is located over the embedded tissue cavity and communicates with the embedded tissue cavity, an extracellular matrix is injected into the top end of the glue injection opening, the embedded tissue cavity is filled with the extracellular matrix, the micro-fluidic chip base is provided with a sliding groove, a first exchange channel, a second exchange channel, a first liquid storage pool and a second liquid storage pool, the sliding rod ispushed into the bottommost portion of the sliding groove, at the moment, the two sides of the embedded tissue cavity are right opposite to a first exchange channel and a second exchange channel respectively, and the first liquid storage pool, the first exchange channel, the embedded tissue cavity, the second exchange channel and the second liquid storage pool are communicated. According to the invention, stable and reliable perfusion of the leakage-free extracellular matrix can be realized; and barrier-free contact between an extracellular matrix and a cell culture medium can be realized, anduniform and stable fluid characteristics can be realized in the extracellular matrix.

Description

technical field [0001] The invention belongs to a microfluidic chip in the technical field of biomedical engineering, and in particular relates to a sliding microfluidic organ chip and a preparation method thereof. Background technique [0002] Compared with traditional two-dimensional cell culture models and animal models with species differences, the human organ chip integrates human cells and hydrogels into the microfluidic chip to further simulate the construction of multiple controllable microenvironments in the body on the chip. Finally, in vitro realization of specific human organ microtissues with three-dimensional microstructure and microphysiological functions has attracted much attention. Therefore, this technology has been widely used in the fields of disease modeling, drug screening and personalized medicine. [0003] Usually, the three-dimensional culture model based on the perfusion mode is widely used in the construction of human-like organ chips. Its basic s...

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Problems solved by technology

[0007] In summary, the currently reported microfluidic organ chips have certain limitations in terms of reliable extracellular matrix perfusion, uniform distribution of fluid in tissue chambers, and cell / microtissue extraction. A new type of organ chip is urgently needed. Human organ chip design to enhance the stability and flexibility of related operations

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