Molecular marker for human skin aging and regulation target and application thereof

An aging and epidermis technology, applied in the field of biomedicine, can solve problems such as skin aging that needs to be explored

Active Publication Date: 2021-02-12
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many research reports on skin aging, the inner mechanism of human skin aging remains to be explored.

Method used

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  • Molecular marker for human skin aging and regulation target and application thereof
  • Molecular marker for human skin aging and regulation target and application thereof
  • Molecular marker for human skin aging and regulation target and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1. Obtainment of human skin basal cell molecular markers

[0083] 1. Single-cell sequencing of human skin

[0084] One), the acquisition of human skin cell suspension

[0085] According to the distribution of human skin age, the young group was composed of 3 volunteer skin samples aged 18-23, the middle-aged group was composed of 3 volunteer samples aged 44-48, and the aged group was composed of 3 volunteer samples aged 70-76. old group. All of them have no skin-related diseases, and all belong to healthy individuals, that is, individuals who have excluded skin cancer, dandruff and other skin-related diseases. Informed consent was obtained from all groups of people in this experiment.

[0086] Obtain cell suspensions from each individual skin. Specific steps are as follows:

[0087] 1. Remove the skin tissue from the upper eyelid of the person through surgery, put it in normal saline, and then rinse it with PBS 3 times.

[0088] 2. After step 1 is complete...

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PUM

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Abstract

The invention discloses a molecular marker and a regulation target for human skin aging and an application of the molecular marker and the regulation target. The inventor of the invention discovers that COL17A1, IGFBP3, POSTN and CYP27A1 can be used as markers to identify or assist in identifying epidermal basal cells by constructing and analyzing a human skin sequencing library and carrying out immunofluorescence detection; HES1 can be used as a marker for distinguishing senescence fibroblasts and young fibroblasts KLF6, and can be used as a marker for distinguishing senescence epidermal basal cells and young epidermal basal cells. The marker can be used for evaluating skin aging related diseases such as basal cell carcinoma, hypolipemic eczema and the like. The product has important application value.

Description

technical field [0001] The invention relates to molecular markers and regulatory targets of human skin aging and applications thereof in the field of biomedicine. Background technique [0002] The skin is the largest organ of the human body, and it is also the natural barrier of the human body against external stimuli. Skin aging is an important feature of human aging. Skin aging can lead to the occurrence of skin-related diseases, such as lipogenic eczema, melanoma, basal cell tumor and other diseases, which seriously affect people's life and health. Although there have been many research reports on skin aging, the inner mechanism of human skin aging remains to be explored. Therefore, an in-depth analysis of the cellular and molecular mechanisms of the skin aging process can provide important intervenable target cells and key regulatory molecules for delaying skin aging and the decline in skin aging-related diseases, and then provide intervention strategies for delaying s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876G01N33/68A61K31/352A61K31/155A61K31/375A61P17/18
CPCC12Q1/6876G01N33/68A61K31/352A61K31/155A61K31/375A61P17/18C12Q2600/158
Inventor 刘光慧曲静张维绮邹治然龙笑赵倩郑彦东宋默识王思
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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