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A strain of Lactobacillus longum sf-b-27 and its application
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A technology of SF-B-27 and Lactobacillus bifidum, applied in the field of microorganisms, can solve the problems of increasing sleep time and high adhesion, and achieve the effects of increasing sleep time, high adhesion and relieving mental fatigue.
Active Publication Date: 2022-07-05
SHANDONG SUNFLOWER BIOENGINEERING CO LTD
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Problems solved by technology
[0004] Aiming at the technical problem that the existing technology lacks products for improving sleep quality that do not cause drug dependence or adverse reactions, the present invention provides a strain of Lactobacillus longum SF-B-27 and its application, Lactobacillus longum SF-B -27 has high adhesion and strong gastrointestinal adaptability, providing a sleep-improving product that can significantly relieve sleep problems and increase sleep time
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Embodiment 1
[0039] Example 1 Isolation and identification of Bifidobacterium longum SF-B-27
[0040] 1. Bacteria source
[0041] Healthy baby stool.
[0042] 2. Isolation of strains
[0043] The fecal samples of healthy infants were diluted ten-fold, and 100 μL of each gradient was evenly spread on the MRS solid plate medium, then taken out and placed in an anaerobic tank, and cultured overnight at 37°C;
[0044] MRS solid plate medium includes the following components:
[0046] The next day, there were colonies of different shapes and sizes on the plate. Pick a number of characteristic colonies of lactic acid bacteria for the second generation streaking purification treatment, and the plate was anaerobic activated for 1 day to obtain the culture, and then the strain id...
Embodiment 2
[0057] Embodiment 2 A kind of product for improving sleep rich in Bifidobacterium longum SF-B-27
[0058] A sleep-improving product rich in Bifidobacterium longum SF-B-27, the product contains Bifidobacterium longum SF-B-27, red date powder, astragalus powder, Codonopsis powder, gastrodia elata powder, isomalt oligosaccharide, among which long double The content of Fidobacteria SF-B-27 was 20 billion / g.
[0059] The preparation method of the product includes the following steps:
[0060] (1) Inoculate Bifidobacterium longum SF-B-27 into the MRS medium plate, the inoculation amount is 1%, after culturing at 37 ° C, centrifuge the MRS bacterial liquid, vacuum freeze-drying, and obtain bacterial powder;
[0061] (2) The auxiliary materials are compounded by red jujube powder, astragalus powder, Codonopsis powder, Gastrodia elata powder and isomalt oligosaccharide in a ratio of 1:1:1:1:1 to obtain a product.
[0062] (3) Mix the Bifidobacterium longum SF-B-27 bacteria powder pre...
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Abstract
The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus Bifidobacterium longum SF-B-27 and its application; the Lactobacillus Bifidobacterium longum preservation number is: CGMCC No.20925; the Bifidobacterium longum SF-B -27 has high adhesiveness and strong gastrointestinal adaptability, can obviously relieve sleep problems and increase sleep time; the product provided by the present invention containing Lactobacillus longum SF-B-27 bacteria powder can improve sleep through Regulate the secretion of tryptophan and serotonin to improve symptoms such as central neurasthenia and insomnia, relieve mental fatigue, inhibit symptoms of anxiety and depression, and adjust the balance of intestinal flora.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus bifidum longum SF-B-27 and its application. Background technique [0002] According to the definition of "Guidelines for the Diagnosis and Treatment of Insomnia in Chinese Adults", insomnia refers to a subjective experience of patients who are not satisfied with sleep time and / or quality and affect daytime social function. Common symptoms of insomnia are difficulty falling asleep, decreased sleep quality and sleep time, and decreased memory and concentration. With the development of society, people's pressure in work and life is also increasing. Due to people's living habits and living environment and other factors, sleep is affected, which directly leads to lack of sleep. At present, nearly 3 billion people in the world suffer from insomnia. , my country also has 600 million people suffering from insomnia, insomnia has become a huge hidden danger...
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