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Method for taking roots and forming rhizomes of tissue culture seedlings of rhizoma smilacis glabrae

A technology of rhizome and tuckahoe, applied in the field of tuckahoe tissue culture seedlings rooting and forming rhizomes, can solve the problems of death, unsustainable utilization, low survival rate and the like

Active Publication Date: 2021-03-09
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the large-scale production of artificial cultivation of Smilax smilax has not been realized, so the problem of its unsustainable utilization is becoming more and more serious
[0004] Smilax cocos has seeds, and the germination rate of the seeds is very high, but they will continue to die during the seedling cultivation process and after transplanting into the ground, and the final survival rate is very low, so it is impossible to provide seedlings for large-scale planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for rooting rhizomes of Smilax cocos tissue culture seedlings, comprising the following steps:

[0020] (1) On the ultra-clean workbench, cut the proliferating clustered buds of Smilax smilax into 3 to 5 buds into a small cluster, and put them into the bud growth medium. After 10 days, the slender buds gradually become thicker. After 30 days, The buds grow very thick, the internodes are very short, and the leaves of most of the buds are densely overlapped;

[0021] The medium for strong buds is: MS+ 6-BA 0.5 mg / L+ IAA0.05 mg / L +PP 333 5.0 mg / L + 30g / L sucrose + 5 g / L agar, pH 6.0;

[0022] The culture conditions are: the culture temperature is 27±2℃, the light time is 12 h / d, and the light intensity is 3000lx;

[0023] (2) On the ultra-clean workbench, cut the thick cluster buds obtained in step (1) into single buds, and insert them into the rooting rhizome induction medium. After 15 days of cultivation, the base of the buds began to expand, and after 25 days...

Embodiment 2

[0027] A method for rooting rhizomes of Smilax cocos tissue culture seedlings, comprising the following steps:

[0028] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Smilax smilax into 3 to 5 buds into a small cluster, and put them into the bud growth medium. After 15 days, the slender buds gradually become thicker. After 30 days, The buds are generally thick and strong, most of the buds grow leaves, and the internodes are longer;

[0029] The medium for strong buds is: MS+ 6-BA 2.0 mg / L+ IAA0.5 mg / L +PP 333 1.0 mg / L + 30 g / L sucrose + 5 g / L agar, pH 6.0;

[0030] (2) On the ultra-clean workbench, cut the thick cluster buds obtained in step (1) into single buds, and insert them into the rooting rhizome induction medium. After culturing for 8 days, the base of the buds began to expand. After 18 days, the enlarged The base tissue sprouted, and the average number of sprouts per plant was about 4.67; at 30 days, the top of the buds expanded to form rhiz...

Embodiment 3

[0034] A method for rooting rhizomes of Smilax cocos tissue culture seedlings, comprising the following steps:

[0035] (1) On the ultra-clean workbench, cut the proliferating clustered buds of Smilax smilax into 3 to 5 buds into a small cluster, and put them into the bud growth medium. After 11 days, the slender buds gradually become thicker. After 30 days, The buds grow thickly, and most of the buds grow leaves, the length of the internodes is appropriate, and the growth of the buds is normal;

[0036] The medium for strong buds is: MS+ 6-BA 1.0 mg / L+ IAA0.2 mg / L +PP 333 2.0 mg / L + 30 g / L sucrose + 5 g / L agar, pH 6.0;

[0037] (2) On the ultra-clean workbench, cut the thick cluster buds obtained in step (1) into single buds, and insert them into the rooting rhizome induction medium. After culturing for 10 days, the base of the buds began to expand, and after 20 days, the enlarged The base tissue sprouted, and the average number of buds per plant was about 5.46; at 30 days, t...

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Abstract

The invention discloses a method for taking roots and forming rhizomes of tissue culture seedlings of rhizoma smilacis glabrae. The method comprises the following steps: firstly, on an ultra-clean workbench, proliferated cluster buds of rhizoma smilacis glabrae are cut into 3-5 buds as a small cluster, and a bud strengthening culture medium is inoculated with the small clusters for culture to obtain strong cluster buds; and the obtained strong cluster buds are cut into single buds, and a rooting and rhizome forming induction culture medium is inoculated with the single buds for culture, wherein in 55-65 days, each seedling has 2-10 roots and 1-7 rhizomes, and in different treatment, each seedling has 2.79-7.33 roots and 1.53-5.46 rhizomes on average. According to the method, the smilacis glabrae buds can form rhizomes while taking roots, two organs including the roots and the rhizomes are induced by one formula, and the rhizomes are medicinal organs of the rhizoma smilacis glabrae, sothat the transplanting survival rate of the rhizoma smilacis glabrae is increased, and the purpose of increasing the yield of the rhizoma smilacis glabrae can be achieved by increasing the rhizomes. The method has the advantages of simple operation, good repeatability, cost reduction, safety, high efficiency and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for rooting and forming rhizomes of soil tuckahoe tissue cultured seedlings. Background technique [0002] Smilax smilax is a perennial plant of the genus Smilax in the family Liliaceae. Its dry rhizome is a traditional Chinese medicinal material recorded in the Pharmacopoeia of the People's Republic of China. It has the effects of dehumidification, detoxification, and tonic joints. In the "Notice on Further Regulating the Management of Health Food Raw Materials" published by the Ministry of Health in recent years, Smilax trichosa is listed as a large number of Chinese herbal medicines that can be used in health food. [0003] Smilax smilax is distributed in Gansu (southern), south of the Yangtze River Basin, Taiwan, Hainan and other provinces. Wild grows in understory, bushes, forest margins, river banks or valleys below 2000m above sea level. Although Smilax ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 石云平许娟苏祖祥李小泉
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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