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A kind of method of soil tuckahoe tissue culture seedling rooting rhizome

A technology for rhizomes and Poria cocos, which is applied to the field of Tuckahoe tuckahoe tissue culture seedlings rooting and forming rhizomes, can solve the problems of low survival rate, inability to provide seedlings for large-scale planting, unsustainable utilization, etc.

Active Publication Date: 2021-05-11
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the large-scale production of artificial cultivation of Smilax smilax has not been realized, so the problem of its unsustainable utilization is becoming more and more serious
[0004] Smilax cocos has seeds, and the germination rate of the seeds is very high, but they will continue to die during the seedling cultivation process and after transplanting into the ground, and the final survival rate is very low, so it is impossible to provide seedlings for large-scale planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings, comprising the following steps:

[0020] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Poria cocos into a small cluster of 3 to 5 buds, and insert them into the strong bud medium. After 10 days, the slender buds gradually become thicker, and after 30 days, The buds are very stout, the internodes are short, and most of the leaves from the buds are densely overlapped;

[0021] Described strong bud medium is: MS+ 6-BA 0.5 mg / L+ IAA 0.05 mg / L +PP 333 5.0 mg / L + sucrose 30 g / L + agar 5 g / L, pH 6.0;

[0022] The culture conditions were: the culture temperature was 27±2°C, the light time was 12 h / d, and the light intensity was 3000lx;

[0023] (2) On the ultra-clean workbench, cut the thick clump buds obtained in step (1) into single buds, and insert them into the rooting and rhizome induction medium. The basal tissue buds, the average number of buds is about 1...

Embodiment 2

[0027] A method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings, comprising the following steps:

[0028] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Poria cocos into a small cluster of 3 to 5 buds, and insert them into the strong bud medium. At 15 days, the slender buds gradually become thicker, and at 30 days, The buds are generally sturdy, most of the buds grow leaves, and the internodes are longer;

[0029] Described strong bud medium is: MS+ 6-BA 2.0 mg / L+ IAA 0.5 mg / L +PP 333 1.0 mg / L + sucrose 30 g / L + agar 5 g / L, pH 6.0;

[0030] (2) On the ultra-clean workbench, cut the thick clump buds obtained in step (1) into single buds, and insert them into the rooting and rhizome induction medium. The basal tissue buds, and the average number of buds per plant is about 4.67; on the 30th day, the top of the bud swells to form a rhizome, and the rhizome is small; on the 40th day, the end of the rhizome that is not connec...

Embodiment 3

[0034] A method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings, comprising the following steps:

[0035] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Poria cocos into a small cluster of 3 to 5 buds, and insert them into the strong bud medium. After 11 days, the slender buds gradually became thicker, and after 30 days, The buds grow thick and strong, and most of the buds grow leaves, the length of the internodes is appropriate, and the growth of the buds is normal;

[0036] Described strong bud medium is: MS+ 6-BA 1.0 mg / L+ IAA 0.2 mg / L +PP 333 2.0 mg / L + sucrose 30 g / L + agar 5 g / L, pH 6.0;

[0037] (2) On the ultra-clean workbench, cut the thick clump buds obtained in step (1) into single buds, and insert them into the rooting and rhizome induction medium. The basal tissue buds, and the average number of buds per plant is about 5.46; at 30 days, the top of the buds swelled to form rhizomes, and the rhizomes were large...

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Abstract

The invention discloses a method for rooting and forming rhizomes for tissue culture seedlings of Poria cocos Cultivate in strong bud medium to obtain thick clump buds; then cut the obtained thick clump buds into single buds, insert them into rooting rhizome induction medium and cultivate, at 55 to 65 days, the number of roots per seedling is 2 ~10, the number of rhizomes was 1-7, the average number of roots per seedling in different treatments was 2.79-7.33, and the average number of rhizomes per plant was 1.53-5.46. The method of the invention can directly form rhizomes when the tuckahoe shoots take root, and one formula induces two organs, the root and the rhizome, and the rhizome is the medicinal organ of the tuckahoe, which not only improves the transplanting survival of the tuckahoe It can also increase the yield of Poria cocos by increasing the number of rhizomes. The method has the advantages of simple operation, good repeatability, low cost, safety and high efficiency.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings. Background technique [0002] Tuckahoe is a perennial plant of the genus Smilax in the Liliaceae family, and its dried rhizomes are Chinese medicinal materials included in the "Pharmacopoeia of the People's Republic of China", which has the effects of dehumidification, detoxification and joint relief. In recent years, the "Notice on Further Standardizing the Management of Raw Materials for Health Foods" published by the Ministry of Health listed Tuckahoe as a bulk Chinese medicinal material that can be used in health food. [0003] Tuckahoe is distributed in Gansu (southern), south of the Yangtze River Basin, as well as in Taiwan, Hainan and other provinces. Wild grows below 2000m above sea level in forests, bushes, forest edges, river banks or valleys. Although Tuckahoe has a wide distribution...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 石云平许娟苏祖祥李小泉
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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