A kind of method of soil tuckahoe tissue culture seedling rooting rhizome
A technology for rhizomes and Poria cocos, which is applied to the field of Tuckahoe tuckahoe tissue culture seedlings rooting and forming rhizomes, can solve the problems of low survival rate, inability to provide seedlings for large-scale planting, unsustainable utilization, etc.
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Embodiment 1
[0019] A method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings, comprising the following steps:
[0020] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Poria cocos into a small cluster of 3 to 5 buds, and insert them into the strong bud medium. After 10 days, the slender buds gradually become thicker, and after 30 days, The buds are very stout, the internodes are short, and most of the leaves from the buds are densely overlapped;
[0021] Described strong bud medium is: MS+ 6-BA 0.5 mg / L+ IAA 0.05 mg / L +PP 333 5.0 mg / L + sucrose 30 g / L + agar 5 g / L, pH 6.0;
[0022] The culture conditions were: the culture temperature was 27±2°C, the light time was 12 h / d, and the light intensity was 3000lx;
[0023] (2) On the ultra-clean workbench, cut the thick clump buds obtained in step (1) into single buds, and insert them into the rooting and rhizome induction medium. The basal tissue buds, the average number of buds is about 1...
Embodiment 2
[0027] A method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings, comprising the following steps:
[0028] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Poria cocos into a small cluster of 3 to 5 buds, and insert them into the strong bud medium. At 15 days, the slender buds gradually become thicker, and at 30 days, The buds are generally sturdy, most of the buds grow leaves, and the internodes are longer;
[0029] Described strong bud medium is: MS+ 6-BA 2.0 mg / L+ IAA 0.5 mg / L +PP 333 1.0 mg / L + sucrose 30 g / L + agar 5 g / L, pH 6.0;
[0030] (2) On the ultra-clean workbench, cut the thick clump buds obtained in step (1) into single buds, and insert them into the rooting and rhizome induction medium. The basal tissue buds, and the average number of buds per plant is about 4.67; on the 30th day, the top of the bud swells to form a rhizome, and the rhizome is small; on the 40th day, the end of the rhizome that is not connec...
Embodiment 3
[0034] A method for rooting and forming rhizomes of Tuckahoe tuckahoe tissue culture seedlings, comprising the following steps:
[0035] (1) On the ultra-clean workbench, cut the proliferating cluster buds of Poria cocos into a small cluster of 3 to 5 buds, and insert them into the strong bud medium. After 11 days, the slender buds gradually became thicker, and after 30 days, The buds grow thick and strong, and most of the buds grow leaves, the length of the internodes is appropriate, and the growth of the buds is normal;
[0036] Described strong bud medium is: MS+ 6-BA 1.0 mg / L+ IAA 0.2 mg / L +PP 333 2.0 mg / L + sucrose 30 g / L + agar 5 g / L, pH 6.0;
[0037] (2) On the ultra-clean workbench, cut the thick clump buds obtained in step (1) into single buds, and insert them into the rooting and rhizome induction medium. The basal tissue buds, and the average number of buds per plant is about 5.46; at 30 days, the top of the buds swelled to form rhizomes, and the rhizomes were large...
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