Application of cFAM210A in preparation of kit and drug for liver cancer diagnosis or postoperative prediction

A technology for diagnostic kits and diagnostic reagents, applied in the directions of drug combination, recombinant DNA technology, DNA/RNA fragments, etc., can solve the problem that the occurrence and development mechanism has not yet been reported, and achieves a simple detection method, and improves the detection rate and accuracy. , the effect of short cycle

Active Publication Date: 2021-03-16
THE NAVAL MEDICAL UNIV OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the occurrence and development mechanism of FA

Method used

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  • Application of cFAM210A in preparation of kit and drug for liver cancer diagnosis or postoperative prediction
  • Application of cFAM210A in preparation of kit and drug for liver cancer diagnosis or postoperative prediction
  • Application of cFAM210A in preparation of kit and drug for liver cancer diagnosis or postoperative prediction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Validation of the ring structure of cFAM210A

[0044] 1.1 Extraction of total RNA from liver tissue

[0045] Take a mung bean-sized liver tissue, add an appropriate amount (usually 1mL) of RNAiso Plus and homogenate; let stand at room temperature for 5 minutes, then centrifuge at 12000g / min, 4°C for 5 minutes; transfer the supernatant to a new 1.5mL EP tube , discard the precipitate; add 0.2 times the volume of RNAiso Plus (usually 0.2mL) of chloroform, shake and mix, and let stand at room temperature for 5 minutes; centrifuge at 12000g / min, 4°C for 15 minutes, and transfer the supernatant to Add an equal volume of isopropanol to a new 1.5mLEP tube, let stand at room temperature for 10 minutes; centrifuge at 12000g / min, 4°C for 10 minutes (white precipitate can be seen at the bottom of the tube), discard the liquid, keep the precipitate, add RNAiso Plus, etc. A volume (usually 1ml) of 75% ethanol was centrifuged at 7500g / min at 4°C for 5 minutes, the liquid ...

Embodiment 2

[0065] Example 2 Detection of the expression level of cFAM210A in liver cancer and adjacent tissues

[0066] 1. Sample collection

[0067] 80 pairs of liver cancer and paracancerous tissue samples were collected and placed in a -80°C refrigerator. All cases did not receive radiotherapy and chemotherapy before surgery, all patients signed informed consent, and the experimental protocol was approved by the ethics committee of the unit.

[0068] The extraction, reverse transcription and real-time quantitative PCR of liver tissue RNA are as described in Example 1. By detecting the expression of cFAM210A in 80 pairs of liver cancer and adjacent tissues, it was found that the expression of cFAM210A in liver cancer tissues was significantly lower than that of adjacent tissues ( figure 2 A, Wilcoxon signed-rank test, P<0.001). The effect of cFAM210A expression in liver tissue in diagnosing liver cancer is expressed as 0.751 (0.677-0.826) by the area under the ROC curve (receiver o...

Embodiment 3

[0069] Example 3 The predictive effect of cFAM210A on the prognosis of patients with liver cancer

[0070] The extraction, reverse transcription and real-time quantitative PCR of liver tissue RNA are as described in Example 1. We detected the expression of cFAM210A in 80 pairs of liver cancer and adjacent tissues, and found that the overall survival rate of liver cancer patients with low cFAM210A expression was significantly lower than that of liver cancer patients with high cFAM210A expression ( image 3 A, Log-rank, P=0.019), the recurrence-free survival rate of liver cancer patients with low expression of cFAM210A was also significantly lower than that of liver cancer patients with high expression of cFAM210A ( image 3 B, Log-rank, P=0.031).

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Abstract

The invention relates to the technical field of medical biological detection, provides new application of cFAM210A, and particularly relates to application of cFAM210A in preparation of liver cancer diagnosis kits and postoperative prediction kits and application of cFAM210A in preparation of drugs for treating liver cancer and anti-liver injury drugs, in particular to application of cFAM210A in preparation of anti-liver injury drugs. The invention further provides a kit for carrying out liver cancer diagnosis or postoperative prediction by utilizing quantitative PCR detection aiming at the cFAM210A. The kit and the detection method are simple, convenient, reliable, short in period, high in specificity and easy to popularize clinically.

Description

technical field [0001] The invention relates to the technical fields of medical biological detection and biopharmaceuticals, and relates to the application of circular RNA-cFAM210A as a molecular marker in the diagnosis of liver cancer, and in particular to the application of cFAM210A in lncRNA in the preparation of liver cancer diagnostic reagents or kits. Background technique [0002] Hepatocellular carcinoma (HCC, hereinafter referred to as liver cancer) is a malignant tumor with the highest morbidity and mortality in my country, and is one of the main disease burdens in China. At present, the commonly used treatment methods for liver cancer include surgery, radiotherapy, chemotherapy, and targeted therapy, but the above-mentioned treatment methods are still limited for the treatment of advanced liver cancer. [0003] The discovery of the molecular biological basis in the development of liver cancer and the corresponding diagnostic and therapeutic molecular markers have i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11A61K45/00A61K48/00A61K31/713A61P35/00A61P1/16
CPCC12Q1/6886C12Q1/6851A61K45/00A61K31/713A61P35/00A61P1/16C12Q2600/118C12Q2600/158C12Q2600/178C12Q2600/136C12Q2531/113C12Q2537/16
Inventor 余建杨富李雯周伟平丁文斌孙大鹏葛春梅戴智慧张潇文
Owner THE NAVAL MEDICAL UNIV OF PLA
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