Traditional Chinese medicine extract composition for treating intestinal cancer, preparation method and application of traditional Chinese medicine extract composition in preparation of drugs
A technology for extracts and compositions is applied in the field of compositions of traditional Chinese medicine extracts to achieve the effects of reducing clinical dosage, improving preparation performance and reducing toxic and side effects
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Embodiment 1
[0020] Example 1 Preparation of Traditional Chinese Medicine Extract
[0021] 1, drug feedstock
[0022] Squirting, gallbladder.
[0023] 2, preparation method for extractance
[0024] Squirting extract: the bitter ginseng medicinal material is pulverized into 12-tit-coated powder, soaking for 1 hour, water extraction 3 times, extracting temperature 80 ~ 90 ° C, 1 hour, 10 times water, and the stirring speed is 20 rpm during extraction. Combined extract, filtration, 3000 r / min centrifuge for 10 minutes, take the supernatant, concentrated to 1 g of halogen, spare;
[0025] Dragon extract: the long gallbladder material is pulverized into 12th glue powder, soaking for 1 hour, water extraction 3 times, extracting temperature 80 ~ 90 ° C, 1 hour, 10 times water, and the stirring speed is 20 rpm during extraction. Combined extract, filtration, 3000 r / min centrifuged for 10 minutes, take the supernatant, concentrated to 1 g of halody, spare.
Embodiment 2
[0026] Example 2 Extract Combination Pharmaceutical on the synergistic effect of inhibiting intestinal cancer cells
[0027] 1, experimental materials
[0028] (1) Drugs: bitter ginseng extract, galloyer extract.
[0029] (2) Intestinal cancer cells: human intestinal cancer cells HCT116 cells, HT29 cells.
[0030] (3) MTT kit.
[0031] 2, experiment group
[0032] (1) The concentration gradient of the bitter ginseng extract.
[0033] (2) The galloyer extract concentration gradient.
[0034] (3) Comprehensive combination of bitter ginsets and galloyer extracts.
[0035] 3, MTT method to detect the sensitivity of tumor cells to different Chinese medicine extracts
[0036] (1) HCT116 cells in human intestinal cancer cells, HT29 cells are inoculated with 5000 to 9000 cells per well to 96-well plates, and after the cells are attached, the bitter ginseng extracts of different pharmaceutical concentrations are added, and the gallbladder extracts are added. Or combined PBS solutions. Afte...
Embodiment 3
[0038] Example 3 Effect of Different Combined Components of Traditional Chinese Medicine Extract on Tumor Cell Inhibition
[0039] 1, experimental materials
[0040] (1) Drugs: bitter ginseng extract, galloyer extract.
[0041] (2) Intestinal cancer cells: human intestinal cancer cells HCT116 cells, HT29 cells.
[0042] (3) MTT kit.
[0043] 2, experiment group
[0044] Phase of pseudo-extracts and galloyer extracts.
[0045] 3, MTT method to detect the sensitivity of tumor cells to different Chinese medicine extracts
[0046] (1) HCT116 cells in human intestinal cancer cells, HT29 cells are inoculated with 5,000 to 9000 cells per well to 96-well plates, and after the cells are attached to the wall, they are added to the piratory substances and gallbladder extracts of different pharmaceutical concentrations, respectively. Combined PBS solution. After 24 h, 10 μl of the concentration of 5 mg / ml of MTT was added to a MTT recharge for 4 h, and then 100 μl of DMSO was added per well...
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