Aryl sulfatase inhibitor as well as preparation method and application thereof

An aryl sulfatase and inhibitor technology, applied in the medical field, can solve the problems of side effects, short drug effect, high price and the like, and achieve the effects of simple operation, good effect and low price

Inactive Publication Date: 2021-03-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of effectively inhibiting arylsulfatase is to use STX64 inhibitor, but it is mostly used in the medical field, and there are disadvantages such as high price, short drug effect and side effects

Method used

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  • Aryl sulfatase inhibitor as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 3 mL of Tris-HCl buffer solution (pH=5.8) in a glass test tube, add 5.22 U of arylsulfatase to the glass test tube, and then add a sodium dodecyl sulfate solution with a concentration of 1 mol / L, so that Tris -The concentration of sodium dodecyl sulfate in the HCl buffer solution is 100 μmol / L, then add the p-nitrophenol sulfuric acid conjugate according to the standard of 800 μmol / L, place the glass test tube in a 37°C water bath for 1 hour, and then use 2 mL of 0.5 mol / L sodium hydroxide was used to terminate the reaction, and the activity of arylsulfatase was determined by standard method (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34:225-229).

Embodiment 2

[0023] Take 3 mL of Tris-HCl buffer solution (pH=5.8) in a glass test tube, add 5.22 U of arylsulfatase to the glass test tube, and then add a sodium dodecyl sulfate solution with a concentration of 1 mol / L, so that Tris -The concentration of sodium dodecyl sulfate in the HCl buffer solution is 120 μmol / L, then add p-nitrophenol sulfuric acid conjugate according to the standard of 800 μmol / L, place the glass test tube in a water bath at 37°C for 1 hour, and then use 2 mL of 0.5 mol / L sodium hydroxide was used to terminate the reaction, and the activity of arylsulfatase was determined by standard method (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34:225-229).

Embodiment 3

[0025] Take 3 mL of Tris-HCl buffer solution (pH=5.8) in a glass test tube, add 5.22 U of arylsulfatase to the glass test tube, and then add a sodium dodecyl sulfate solution with a concentration of 1 mol / L, so that Tris -The concentration of sodium dodecyl sulfate in the HCl buffer solution is 140 μmol / L, then add p-nitrophenol sulfuric acid conjugate according to the standard of 800 μmol / L, place the glass test tube in a water bath at 37°C for 1 hour, and then use 2 mL of 0.5 mol / L sodium hydroxide was used to terminate the reaction, and the activity of arylsulfatase was determined by standard method (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34:225-229).

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Abstract

The invention belongs to the field of medicine, and discloses an aryl sulfatase inhibitor as well as a preparation method and application thereof. Dodecyl sulfate is added into a solvent, so that a solution can be obtained, namely the aryl sulfatase inhibitor can be prepared. Compared with a conventional medical treatment inhibitor STX64, the inhibitor STX64 has the advantages of being low in effective inhibition concentration, capable of achieving inhibition of different degrees according to needs, easy to operate, low in cost, rapid and the like, and has a bright application prospect in thefields of environment and medicine.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an arylsulfatase inhibitor and its preparation method and application. Background technique [0002] Arylsulfatase (arylsulfatase) widely exists in various organs of humans and animals, and also exists in various environmental media such as domestic sewage. The important role of arylsulfatase is to convert sulfate conjugates such as natural estrogen into natural estrogen, that is, desulfate conjugates. In animals, arylsulfatase may be closely related to the normal physiological functions of the human body. For example, some breast cancer patients use drugs to inhibit arylsulfatase, so as to control the progression of breast cancer. At present, the method of effectively inhibiting arylsulfatase is to use STX64 inhibitor, but it is mostly used in the medical field, and has the disadvantages of high price, short drug effect and side effects. Contents of the invention [0003...

Claims

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Application Information

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IPC IPC(8): G01N31/10
CPCG01N31/10
Inventor刘则华张俊钟姝姝党志
OwnerSOUTH CHINA UNIV OF TECH