Probiotic simulant and preparation method thereof

Abstract

The invention belongs to the technical field of preparation of probiotic simulants, and discloses a probiotic simulant and a preparation method thereof. The preparation method of the probiotic simulant comprises the following steps of culturing cells in an improved culture medium for supplementing fetal calf serum; when the cells are in the 30th +/-10th generation, carrying out TEER measurement; after growing for 48 hours, carrying out medium-voltage isolation; washing an aldehyde/sulfate latex bead suspension with high-purity water for three times, and then re-suspending in the high-purity water; and mixing an MV suspension with purified beads and PBS, and preparing MV-coated particles. Through the method, high-concentration delivery of the MVs can be realized. The PBMs prepared by the method are used as an anti-inflammatory preparation, proinflammatory TNF-alpha is remarkably reduced, the PBMs can improve intestinal barrier function loss caused by inflammation, and the withdrawal effect on methamphetamine can be realized by the method, so that positive influence is generated on drug rehabilitation.

Description

technical field [0001] The invention belongs to the technical field of preparation of probiotic simulants, in particular to a probiotic simulant and a preparation method thereof. Background technique [0002] Currently, the problem of drug abuse is very serious. Drug dependence is similar to the neurological principles of substance dependence such as alcohol dependence, and the modulation of gut microbiota with probiotics or prebiotics is a promising and safe approach for the treatment of substance dependence. Prospective, Randomized, placebo-controlled clinical trial. [0003] Intestinal flora changes in methamphetamine addicts, Ning Tingting established a conditioned place preference model for rats dependent on methamphetamine (MA), and then collected the feces of the rats for 16srDNA sequencing and found that in Compared with the gut microbiota of rats addicted to methamphetamine, many specific microbial groups have changed, among which the relative abundance of the ge...

AI Technical Summary

Problems solved by technology

Once these silenced genes are activated by unknown triggers, they may cause pathogenic effects in patients, greatly limiting the safety of probiotics

[0010] Through the above analysis, the existing problems and defects of the prior art are: probiotics can cause bacteremia and sepsis in patients with IBD treated with immunosuppressive agents for a long time; The same genes, once these silenced genes are activated by unknown triggers, they may cause pathogenic effects in patients, greatly limiting the safety of probiotics

[0011] The difficulty in solving the above problems and defects is: due to the proliferative ability of probiotics, they are not always suitable as a treatment agent, especially for patients with long-term use of immunosuppressive drugs for IBD. The use of probiotics can lead to bacteremia and sepsis

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