Preparation method of composite microbial preparation capable of being combined with antibiotics

A technology of complex microorganisms and antibiotics, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as the increase of susceptible and drug-resistant strains, difficulties in disease control and treatment, and low non-specific immunity. The use of science, animal safety, and the effect of promoting animal growth
CN112625975APending Publication Date: 2021-04-09广东辉阳生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广东辉阳生物科技有限公司
Publication Date
2021-04-09

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Abstract

The invention provides a preparation method of a composite microbial preparation capable of being combined with antibiotics. The method comprises the following preparation steps of: step 1, activating lactobacillus acidophilus freeze-dried powder and lactobacillus casei freeze-dried powder twice by using a liquid culture medium at the temperature of 35-40 DEG C respectively to obtain activated lactobacillus acidophilus and lactobacillus casei; step 2, performing streak inoculation on the activated lactobacillus acidophilus and lactobacillus casei to a solid culture medium respectively to grow single bacterial colonies of the lactobacillus acidophilus and lactobacillus casei; step 3, taking 1-2 rings from the single bacterial colonies of the lactobacillus acidophilus and lactobacillus casei in a 50-100ml seed liquid culture medium by using an inoculating ring in a sterile environment to prepare a primary seed solution; and step 4, taking the same-level seed solution of the lactobacillus acidophilus and lactobacillus casei in the single bacterial colonies according to the ratio of 1: 1, and inoculating the same-level seed solution to a 500-1000ml seed liquid culture medium to prepare a secondary seed solution, wherein the inoculation amount of the same-level seed solution is 5-8%.
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Description

technical field

[0001] The invention belongs to the technical field of microbial preparations, and relates to a preparation method of a composite microbial preparation that can be used in combination with antibiotics. Background technique

[0002] In recent years, with the rapid increase in the intensification of the breeding industry, the pressure on disease prevention and control has increased. In order to reduce the incidence of disease and save breeding costs, farmers usually use antibiotics for preventive health care and growth promotion. The long-term abuse of antibiotics has led to low non-specific immunity of animals, and the increase of susceptible and drug-resistant strains, making disease control and treatment more difficult. At the same time, it has also brought safety hazards to human food health and seriously endangered people's health. Contents of the invention

[0003] In order to achieve the above object, the present invention adopts the following technica...

Claims

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