Preparation method of composite microbial preparation capable of being combined with antibiotics

A technology of complex microorganisms and antibiotics, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as the increase of susceptible and drug-resistant strains, difficulties in disease control and treatment, and low non-specific immunity. The use of science, animal safety, and the effect of promoting animal growth

Pending Publication Date: 2021-04-09
广东辉阳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long-term abuse of antibiotics has led to low non-specific immunity of animals, and the increase of susceptible and drug-resistant strains, making disease control and treatment more difficult. At the same time, it also brings safety hazards to human food health and seriously endangers people's health.

Method used

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  • Preparation method of composite microbial preparation capable of being combined with antibiotics
  • Preparation method of composite microbial preparation capable of being combined with antibiotics
  • Preparation method of composite microbial preparation capable of being combined with antibiotics

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preparation example Construction

[0022] In the embodiment of the present invention, the preparation method of the composite microbial preparation that can be used in combination with antibiotics comprises the following preparation steps:

[0023] Step 1: Activate the freeze-dried bacteria powder of Lactobacillus acidophilus and the freeze-dried bacteria powder of Lactobacillus casei with liquid medium respectively at a temperature of 35°C to 40°C twice; obtain activated Lactobacillus acidophilus and cheese milk Bacillus;

[0024] Step 2: Streak inoculate the activated Lactobacillus acidophilus and Lactobacillus casei on the solid medium respectively, and then place the solid medium in a temperature environment of 35°C-40°C for 24-30h to grow acidophilus Single colonies of Lactobacillus and Lactobacillus casei;

[0025] Step 3: In a sterile environment, use an inoculation loop to take 1-2 loops from a single colony of Lactobacillus acidophilus and Lactobacillus casei, respectively, and place them in 50ml-100m...

Embodiment 1

[0031] The preparation method of the composite microbial preparation that can be used in combination with antibiotics comprises the following preparation steps:

[0032] Step 1: Activate the freeze-dried bacteria powder of Lactobacillus acidophilus and the freeze-dried bacteria powder of Lactobacillus casei with liquid medium respectively at a temperature of 35°C to 40°C twice; obtain activated Lactobacillus acidophilus and cheese milk Bacillus;

[0033] Step 2: Streak inoculate the activated Lactobacillus acidophilus and Lactobacillus casei on the solid medium respectively, and then place the solid medium in a temperature environment of 35°C-40°C for 24-30h to grow acidophilus Single colonies of Lactobacillus and Lactobacillus casei;

[0034] Step 3: In a sterile environment, use an inoculation loop to take 1-2 loops from a single colony of Lactobacillus acidophilus and Lactobacillus casei, respectively, and place them in 50ml-100ml seed liquid medium, and then place them at...

Embodiment 2

[0040] The preparation method of the composite microbial preparation that can be used in combination with antibiotics comprises the following preparation steps:

[0041] Step 1: Activate the freeze-dried bacteria powder of Lactobacillus acidophilus and the freeze-dried bacteria powder of Lactobacillus casei with liquid medium respectively at a temperature of 35°C to 40°C twice; obtain activated Lactobacillus acidophilus and cheese milk Bacillus;

[0042] Step 2: Streak inoculate the activated Lactobacillus acidophilus and Lactobacillus casei on the solid medium respectively, and then place the solid medium in a temperature environment of 35°C-40°C for 24-30h to grow acidophilus Single colonies of Lactobacillus and Lactobacillus casei;

[0043] Step 3: In a sterile environment, use an inoculation loop to take 1-2 loops from a single colony of Lactobacillus acidophilus and Lactobacillus casei, respectively, and place them in 50ml-100ml seed liquid medium, and then place them at...

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Abstract

The invention provides a preparation method of a composite microbial preparation capable of being combined with antibiotics. The method comprises the following preparation steps of: step 1, activating lactobacillus acidophilus freeze-dried powder and lactobacillus casei freeze-dried powder twice by using a liquid culture medium at the temperature of 35-40 DEG C respectively to obtain activated lactobacillus acidophilus and lactobacillus casei; step 2, performing streak inoculation on the activated lactobacillus acidophilus and lactobacillus casei to a solid culture medium respectively to grow single bacterial colonies of the lactobacillus acidophilus and lactobacillus casei; step 3, taking 1-2 rings from the single bacterial colonies of the lactobacillus acidophilus and lactobacillus casei in a 50-100ml seed liquid culture medium by using an inoculating ring in a sterile environment to prepare a primary seed solution; and step 4, taking the same-level seed solution of the lactobacillus acidophilus and lactobacillus casei in the single bacterial colonies according to the ratio of 1: 1, and inoculating the same-level seed solution to a 500-1000ml seed liquid culture medium to prepare a secondary seed solution, wherein the inoculation amount of the same-level seed solution is 5-8%.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and relates to a preparation method of a composite microbial preparation that can be used in combination with antibiotics. Background technique [0002] In recent years, with the rapid increase in the intensification of the breeding industry, the pressure on disease prevention and control has increased. In order to reduce the incidence of disease and save breeding costs, farmers usually use antibiotics for preventive health care and growth promotion. The long-term abuse of antibiotics has led to low non-specific immunity of animals, and the increase of susceptible and drug-resistant strains, making disease control and treatment more difficult. At the same time, it has also brought safety hazards to human food health and seriously endangered people's health. Contents of the invention [0003] In order to achieve the above object, the present invention adopts the following technica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K20/195C12R1/23C12R1/245
CPCC12N1/20A23K10/18A23K20/195A23V2400/125A23V2400/113
Inventor 陈彦豪
Owner 广东辉阳生物科技有限公司
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